L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境

茶叶科学 ›› 2017, Vol. 37 ›› Issue (1): 17-24.

L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境

郭晨^1,2, 刘林峰^1,2, 向奕^1,2, 罗莉^1,2, 林玲^1,2, 肖文军^1,2,3,*

1. 湖南农业大学园艺园林学院茶学教育部重点实验室,湖南长沙 410128;
2. 湖南农业大学国家植物功能成分利用工程技术研究中心,湖南长沙410128;
3. 湖南农业大学湖南省植物功能成分利用协同创新中心,湖南长沙 410128

收稿日期:2016-06-02 修回日期:2016-09-18 出版日期:2017-02-15 发布日期:2019-08-22
通讯作者: *xiaowenjun88@sina.com
作者简介:郭晨,女,硕士研究生,主要从事茶叶功能成分的利用研究。
基金资助:
国家“十二五”科技支撑计划项目（2011BAD10B00）

L-theanine Attenuates Tissue and Oxidative Damages in Heat Stressed Mice

GUO Chen^1,2, LIU Linfeng^1,2, XIANG Yi^1,2, LUO Li^1,2, LIN Ling^1,2, XIAO Wenjun^1,2,3,*

1. Key Lab of Tea Science of Ministry of Education,
2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,
3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China

Received:2016-06-02 Revised:2016-09-18 Online:2017-02-15 Published:2019-08-22

摘要/Abstract

摘要： 以SPF级Slca/KM雄性小鼠作为实验对象,在恒温恒湿模拟气候箱进行热处理,灌喂干预不同剂量L-茶氨酸,考察L-茶氨酸对小鼠采食量、体重变化、空肠切片病理情况、器官指数、血清谷丙转氨酶（ALT）与谷草转氨酶（AST）活性、血清肿瘤坏死因子（TNF-α）、白细胞介素6（IL-6）和干扰素-γ（IFN-γ）含量、肝组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性以及肝组织病理学变化的影响,以探讨L-茶氨酸改善热处理引起的小鼠组织损伤和氧化逆境作用。结果表明,L-茶氨酸能在一定程度上促进热处理小鼠采食量和体重的增加,降低肝脏和脾脏器官指数,降低血清ALT、AST酶活性,抑制血清炎症介质TNF-α、IL-6、IFN-γ的含量,降低肝组织MDA含量,提高SOD、GSH-Px、CAT酶活性,减轻热处理对空肠及肝脏组织的损伤,且以L-茶氨酸中剂量处理效果较好。说明L-茶氨酸具有改善热处理引起的小鼠组织损伤和氧化逆境的作用,主要表现为提高小鼠营养物质吸收能力、减少炎性细胞因子表达,以及降低氧化损伤。

关键词: L-茶氨酸, 热处理, 组织损伤, 氧化逆境

Abstract: The effects of different dosages of L-theanine on SPF grade Balb/KM male mice under heat treatment, including feed intake, weight, organs index, histopathological features of jejunum, activities of serum alanine aminotranferease (ALT) and aspartate aminotransferase (AST), contents of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interferon-γ (IFN-γ) in serum, content of liver homogenate malondialdehyde (MDA), activities of liver homogenate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and histopathological features of liver tissue were studied. Results showed that pretreatment with different dosages of L-theanine increased the feed intake and body weight of the mice, enhanced the activities of liver homogenate SOD, CAT, GSH-Px, decreased the index of liver and spleen, damages in the jejunum, activities of serum ALT, AST, contents of TNF-α, IL-6 and IFN-γ in serum, content of liver homogenate MDA and attenuated the damages of liver by heat treatment. The best attenuating effect of L-theanine on damages was under middle dosage treatment. It suggests that L-theanine mainly improves the absorptive capacity, reduces the expressions of inflammatory cytokines and oxidative damage in mice, which finally attenuate the tissue and oxidative damages caused by heat stress.

Key words: L-theanine, heat stress, tissue damage, oxidative stress

中图分类号:

TS272
Q51

郭晨, 刘林峰, 向奕, 罗莉, 林玲, 肖文军. L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境[J]. 茶叶科学, 2017, 37(1): 17-24.

GUO Chen, LIU Linfeng, XIANG Yi, LUO Li, LIN Ling, XIAO Wenjun. L-theanine Attenuates Tissue and Oxidative Damages in Heat Stressed Mice[J]. Journal of Tea Science, 2017, 37(1): 17-24.

参考文献 18

[1]	戴四发. 谷氨酰胺和γ-氨基丁酸对肉鸡抗热应激和肉品质的影响及机理探讨[D]. 南京: 南京农业大学, 2012: 1-7.
[2]	周鑫, 黄毅, 李延森, 等. 叔丁基对苯二酚对热应激小鼠肝脏氧化损伤的缓解作用[J]. 动物营养学报, 2014, 26(9): 2779-2788.
[3]	Liu LL, He JH, Xie HB, et al.Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens[J]. Poultry Science, 2014, 93(1): 54-62.
[4]	胡娟, 陈继兰, 文杰, 等. 急性热应激对北京油鸡生产性能的影响及Vc添加效应的研究[J]. 安徽农业大学学报, 2010, 37(3): 440-444.
[5]	何钦. 热应激对不同泌乳阶段奶牛生产性能及其营养代谢的影响[D]. 重庆: 西南大学, 2012: 7-9.
[6]	潘晓建, 彭增起, 周光宏, 等. 宰前热应激对肉鸡胸肉氧化损伤和蛋白质功能特性的影响[J]. 中国农业科学, 2008, 41(6): 1778-1785.
[7]	李静, 乔健, 高铭宇, 等. 40℃持续热应激对肉鸡股动脉压的影响[J]. 中国农业大学学报, 2004, 9(3): 41-44.
[8]	罗炳德, 邹飞, 万为人, 等. 复方人参制剂对热应激大鼠的保护作用研究[J]. 中国工业医学杂志, 2001, 14(3): 136-138.
[9]	肖敏华, 唐兴刚, 韩前彪, 等. 中药对热应激动物消化系统作用的研究进展[J]. 中国畜牧兽医, 2010, 37(9): 176-179.
[10]	施忠秋. γ-氨基丁酸调控采食量和缓解热应激的机制[J]. 动物营养学报, 2014, 26(1): 49-53.
[11]	宛晓春. 茶叶生物化学[M]. 北京: 中国农业出版社, 2003: 105.
[12]	Ritsner MS, Miodownik C, Ratner Y, et al.L-theanine relieves positive, activation, and anxiety symptoms in patients with schizophre-nia and schizoaffective disorder: an 8-week, randomized, double-blind, placebo-controlled, 2-center study[J]. J Clin Psychiatry, 2011, 72(1): 34-42.
[13]	Sadzuka Y, Yamashita Y, Kishimoto S, et al.Glutamate transporter mediated increase of antitumor activity by theanine, an amino acid in green tea[J]. The Pharmaceutical Society of Japan, 2002, 122(11): 995-999.
[14]	Kimura K, Ozeki M, Juneja LR, et al.L-Theanine reduces psychological and physiological stress responses[J]. Biological Psychology, 2007, 74(1): 39-45.
[15]	彭彬, 刘仲华, 林勇, 等. L-茶氨酸改善慢性应激大鼠抑郁行为作用研究[J]. 茶叶科学, 2014, 34(4): 355-363.
[16]	陈静, 李丹丹, 邹慧, 等. 富硒女贞子对热应激小鼠内分泌及免疫功能的影响[J]. 畜牧与兽医, 2015, 47(6): 105-108.
[17]	李华. 维生素C稳定性研究[J]. 食品科学, 1989, 10(9): 3-5.
[18]	Yanli Deng, Wenjun Xiao, Ling Chen, et al.In vivo antioxidative effects of L-theanine in the presence or absence of Escherichia coli-induced oxidative stress[J]. Journal of Functional Foods, 2016, 24(4): 527-536.