茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究

doi:10.13305/j.cnki.jts.2016.03.012

茶叶科学 ›› 2016, Vol. 36 ›› Issue (3): 323-329.doi: 10.13305/j.cnki.jts.2016.03.012

茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究

李建宇¹, 史梦竹¹, 傅建炜^1,*, 王婷¹, 张志康²

1. 福建省农业科学院植物保护研究所,福建福州 350013;
2. 湖南农业大学植物保护学院,湖南长沙 410128

收稿日期:2015-11-04 出版日期:2016-06-15 发布日期:2019-08-23
通讯作者: *,fjw9238@163.com
作者简介:李建宇,男,助理研究员,主要从事农业昆虫与害虫防治、农药毒理与生物安全研究。
基金资助:
福建省自然科学基金（2013J01112）

Activities of the Detoxifying Enzymes and Synergism in the Bifenthrin-resistant and Susceptible Strains of Tea Leafhopper, Empoasca vitis

LI Jianyu¹, SHI Mengzhu¹, FU Jianwei^1,*, WANG Ting¹, ZHANG Zhikang²

1. Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China

Received:2015-11-04 Online:2016-06-15 Published:2019-08-23

摘要/Abstract

摘要： 为了明确茶假眼小绿叶蝉（Empoasca vitis）对联苯菊酯代谢抗性相关的解毒酶,本研究采用室内生物测定和生化分析方法,比较了假眼小绿叶蝉抗联苯菊酯品系和敏感品系的3种解毒酶活性差异,并分析了3种增效剂对联苯菊酯的增效作用。解毒酶活性测定表明,茶假眼小绿叶蝉抗联苯菊酯品系羧酸酯酶（CarE）、细胞色素P450氧化酶O-脱甲基（PNOD）、谷胱甘肽-S-转移酶（GST）比活力都高于敏感品系,3种解毒酶活性分别为敏感品系的2.09倍、4.34倍和1.59倍。增效剂磷酸三苯酯（TPP）、增效醚（PBO）和顺丁烯二酸二乙酯（DEM）在茶假眼小绿叶蝉敏感品系中的增效比分别为1.04、1.09、1.00,而在联苯菊酯抗性品系中的增效比分别为1.80、7.97、1.03。上述结果证明,羧酸酯酶和细胞色素P450氧化酶活性的增强在茶假眼小绿叶蝉对联苯菊酯的抗性产生中起到了重要的作用。

关键词: 茶假眼小绿叶蝉, 联苯菊酯, 抗性, 解毒酶, 增效作用

Abstract: Tea green leafhopper, Empoasca vitis, is the most serious pest in tea gardens. The detoxifying enzyme activities and synergism in the bifenthrin-resistant and susceptible leafhopper were measured via biochemical methods and bioassays to clarify their potential relationship to the resistance of tea leafhopper to bifenthrin. The detoxifying enzyme activity tests showed that the activities of carboxylesterase (CarE), Cytochrome P450-mediated O-demethylation activity toward p-nitroanisole (PNOD) and glutathione-S-transferase (GST) in the resistant strain were all higher than those in susceptible strain. The activities of the three detoxifying enzymes in bifenthrin-resistant strain were 2.09, 4.34 and 1.59 folds those of the susceptible strain respectively. Synergism tests showed that the synergist ratios of triphenyl phosphate (TPP), piperonyl buoxide (PBO) and diethyl maleate (DEM) were 1.04, 1.09, and 1.00 folds respectively in the bifenthrin-susceptible strain and 1.80, 7.97 and 1.03 foldsrespectively in the bifenthrin-resistant strain. These results indicate that CarE and PNOD play important roles in bifenthrin resistance of tea leafhoppers.

Key words: Empoasca vitis, bifenthrin, resistance, detoxifying enzymes, synergism

中图分类号:

李建宇, 史梦竹, 傅建炜, 王婷, 张志康. 茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究[J]. 茶叶科学, 2016, 36(3): 323-329. doi: 10.13305/j.cnki.jts.2016.03.012.

LI Jianyu, SHI Mengzhu, FU Jianwei, WANG Ting, ZHANG Zhikang. Activities of the Detoxifying Enzymes and Synergism in the Bifenthrin-resistant and Susceptible Strains of Tea Leafhopper, Empoasca vitis[J]. Journal of Tea Science, 2016, 36(3): 323-329. doi: 10.13305/j.cnki.jts.2016.03.012.

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茶假眼小绿叶蝉抗联苯菊酯品系和敏感品系解毒酶活性及增效作用研究

Activities of the Detoxifying Enzymes and Synergism in the Bifenthrin-resistant and Susceptible Strains of Tea Leafhopper, Empoasca vitis

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