茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析

doi:10.13305/j.cnki.jts.2016.03.011

茶叶科学 ›› 2016, Vol. 36 ›› Issue (3): 312-322.doi: 10.13305/j.cnki.jts.2016.03.011

茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析

李辉^1,3, 黄蔚², 刘志薇^1,3, 王永鑫^1,3, 吴致君^1,3, 庄静^1,3,*

1. 南京农业大学园艺学院, 江苏南京 210095;
2. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京 210095;
3. 南京农业大学茶叶科学研究所,江苏南京 210095

收稿日期:2015-08-04 出版日期:2016-06-15 发布日期:2019-08-23
通讯作者: *,zhuangjing@njau.edu.cn
作者简介:李辉,男,硕士研究生,主要从事茶树分子生物学研究。
基金资助:
国家自然科学基金（31200520、31570691）

Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis

LI Hui^1,3, HUANG Wei², LIU Zhiwei^1,3, WANG Yongxin^1,3, WU Zhijun^1,3, ZHUANG Jing^1,3,*

1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
3. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China

Received:2015-08-04 Online:2016-06-15 Published:2019-08-23

摘要/Abstract

摘要： 茶树是一种重要的经济作物,温度胁迫对茶叶生产影响较大。本研究以茶树品种迎霜为实验材料,克隆获得2个编码Dof转录因子的基因CsDof1和CsDof2。序列分析显示,CsDof1含有1 389 bp,编码463个氨基酸;CsDof2含有1 458 bp,编码486个氨基酸。CsDof1和CsDof2转录因子都具有典型的锌指结构域,分别位于第133~195和124~186个氨基酸位点之间。对CsDof1和CsDof2转录因子理化性质、亲/疏水性、二级和三级结构分析显示,两者都是亲水性蛋白且显偏碱性。空间结构分析显示,CsDof1和CsDof2均有1个α-螺旋,且Dof结构域的位点完全相同,均有4个半胱氨酸残基。实时定量PCR表明,CsDof1和CsDof2在不同茶树品种、不同温度胁迫下均能诱导表达,且表达呈现差异。

关键词: 茶树, Dof转录因子, 锌指结构, 温度胁迫, 基因表达

Abstract: Tea is an important economic crop. Tea production is affected by temperature factors. CsDof1 and CsDof2, which encode Dof transcription factors, were cloned from tea cultivar ‘Yingshuang’ [Camellia sinensis (L.) O. Kuntze]. The nucleotide and amino acid sequences, phylogenetic tree and molecular models of the CsDof1 and CsDof2 were analyzed. The results showed that the CsDof1 and CsDof2 are 1389 and 1458 nucleotides in length, which encode 463 and 486 amino acids respectively. Typical zinc finger domains are present in both CsDof1 and CsDof2, from coden 133 to 195 and 124 to 186 respectively. The CsDof1 and CsDof2 were hydrophilic proteins. The three-dimensional structures of CsDof1 and CsDof2 indicated the presence of a highly conserved Dof domain with four cysteine residues. Quantitative real-time PCR analysis showed that CsDof1 and CsDof2 were induced by different temperature stresses and the expression profiles were different among tea cultivars.

Key words: Camellia sinensis, Dof transcription factor, zinc finger, temperature stress, gene expression

中图分类号:

李辉, 黄蔚, 刘志薇, 王永鑫, 吴致君, 庄静. 茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析[J]. 茶叶科学, 2016, 36(3): 312-322. doi: 10.13305/j.cnki.jts.2016.03.011.

LI Hui, HUANG Wei, LIU Zhiwei, WANG Yongxin, WU Zhijun, ZHUANG Jing. Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis[J]. Journal of Tea Science, 2016, 36(3): 312-322. doi: 10.13305/j.cnki.jts.2016.03.011.

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茶树两个Dof转录因子的分离及其在温度胁迫中的响应分析

Isolation and Expression Analysis of Two Temperature Responsive Dof Genes from Camellia sinensis

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