HPLC-ESI-MS Analysis of Pigments in ZijuanGreen Tea and Its Stability

GONG Jia-shun; SUI Hua-song; PENG Chun-xiu; FAN Jian-gong; LI Ya-li

doi:10.13305/j.cnki.jts.2012.02.006

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (2) : 179-188. DOI: 10.13305/j.cnki.jts.2012.02.006

HPLC-ESI-MS Analysis of Pigments in ZijuanGreen Tea and Its Stability

GONG Jia-shun¹, SUI Hua-song¹, PENG Chun-xiu², FAN Jian-gong¹, LI Ya-li³

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Abstract

The pigments and its stability in Zijuan sun-dried green tea were investigated in this study. The pigments from Zijuan sun-dried green tea were extracted by acidic methanol-H₂O solution and H₂O, and the main compounds were identified by means of HPLC-ESI-MS/MS techniques. 14 anthocyanin, 24 flavonols, 8 phenolics and theaflavins and 3 alkaloids were identified from the extracts of Zijuan sun-dried green tea. The main anthocyanins, such as Cyanidin-3-glucoside, Cyaniding-3-galactoside, Cyanidin-3-O-(6-p-coumaroyl)-glucoside, Delphinidin-3- glucoside, Delphinidin-3-rutinoside, Delphinidin-3-O-(6-p-coumaroyl)-glucoside, Prodelphinidin B-2(or 4)3′-O- gallate, Procyanidin B-2(or 4)3′-O-gallate, Petunidin-3-glc-4-vinylphenol and Malvidin-3-glc-4- vinylepicatechin etc were identified in Zijuan sun-dried green tea. The pigment extracted from Zijuan sun-dried green tea by using acidic methanol-methanol-H₂O as solvent showed good water-solubility and was stable to light and heat in acid environment, but sensitive to the pH value of environment, oxidizer and reducer. The maximum adsorption wavelength for the pigment is 538nm under pH 1.0. Fe²⁺ and Cu²⁺ can change the color of the pigments and stability was decreased. The substance of the purple colour in Zijuan sun-dried green tea was clarified by HPLC-ESI-MS/MS analysis.

Key words

anthocyanins / flavonoids / HPLC-ESI-MS / phenolics / stability / Zijuan sun-dried green tea

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GONG Jia-shun, SUI Hua-song, PENG Chun-xiu, FAN Jian-gong, LI Ya-li. HPLC-ESI-MS Analysis of Pigments in ZijuanGreen Tea and Its Stability[J]. Journal of Tea Science. 2012, 32(2): 179-188 https://doi.org/10.13305/j.cnki.jts.2012.02.006

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