Exploring the Potential Mechanism of Hypoglycemic Effect of Fungus Fermented Black Tea Based on Liver Transcriptomics

ZENG Hongzhe; PENG Liyuan; WAN Liwei; LIU Changwei; FANG Wenwen; WANG Kuofei; ZHANG Xinyi; WEN Shuai; HUANG Jian'an; LIU Zhonghua

doi:10.13305/j.cnki.jts.2023.05.003

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (5) : 645-656. DOI: 10.13305/j.cnki.jts.2023.05.003

Research Paper

Exploring the Potential Mechanism of Hypoglycemic Effect of Fungus Fermented Black Tea Based on Liver Transcriptomics

ZENG Hongzhe, PENG Liyuan, WAN Liwei, LIU Changwei, FANG Wenwen, WANG Kuofei, ZHANG Xinyi, WEN Shuai, HUANG Jian'an^*, LIU Zhonghua^*

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Abstract

EGCG is often regarded as the main active ingredient in tea to protect blood glucose homeostasis. The content of low molecular weight polyphenols such as EGCG in fungus fermented black tea (FFBT) is extremely low, and there are few studies on the hyperglycemic effect of FFBT. To explore the hypoglycemic effects and potential mechanisms of FFBT, GK rats with spontaneous hyperglycemic were given 280 mg·kg^-1 FFBT extract (equivalent to 9 g of FFBT for daily human consumption) by gavage intervention. The study assessed the effects of FFBT on body weight, glucose homeostasis, regulatory factors related to glucose homeostasis, diabetic complications and liver transcription profiles in hyperglycemic rats. The results indicated that FFBT could obviously reduce the fasting blood glucose level and random blood sugar level of hyperglycemic rats, improve the abnormal glucose metabolism in hyperglycemic rats, maintain glucose homeostasis and alleviate the damage caused by diabetic complications in hyperglycemic rats. In addition, transcriptome analysis revealed that the hypoglycemic properties of FFBT might be related to the regulation of gene expression in the liver, such as Gck, Pklr, Pkm. This study found that FFBT may be a potential hypoglycemic functional beverage, providing a theoretical basis for the development and utilization of fermented tea such as FFBT.

Key words

black tea / diabetic complication / GK rats / hypoglycemic effect / transcriptomics

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ZENG Hongzhe, PENG Liyuan, WAN Liwei, LIU Changwei, FANG Wenwen, WANG Kuofei, ZHANG Xinyi, WEN Shuai, HUANG Jian'an, LIU Zhonghua. Exploring the Potential Mechanism of Hypoglycemic Effect of Fungus Fermented Black Tea Based on Liver Transcriptomics[J]. Journal of Tea Science. 2023, 43(5): 645-656 https://doi.org/10.13305/j.cnki.jts.2023.05.003

References

[1] 林海, 丁钢强, 王志宏, 等. 新营养学展望:营养、健康与可持续发展[J]. 营养学报, 2019, 41(6): 521-529.
Lin H, Ding G Q, Wang Z H, et al.The prospective in new nutrition science: nutrition, health and sustainable development[J]. Acta Nutrimenta Sinica, 2019, 41(6): 521-529.
[2] Saeedi P, Petersohn I, Salpea P, et al.Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: results from the International Diabetes Federation Diabetes Atlas[J]. Diabetes Research and Clinical Practice, 2019, 157: 107843. doi: 10.1016/j.diabres.2019.107843.
[3] Chatterjee S, Khunti K, Davies M J.Type 2 diabetes[J]. Lancet, 2017, 389(10085): 2239-2251.
[4] Defronzo R A, Ferrannini E, Groop L, et al.Type 2 diabetes mellitus[J]. Nature Reviews Disease Primers, 2015, 1: 15019. doi: 10.1038/nrdp.2015.19.
[5] 中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2020年版)[J]. 中华内分泌代谢杂志, 2021, 37(4): 311-398.
Chinese Diabetes Society.Guideline for the prevention and treatment of type 2 diabetes mellitus in China (2020 edition)[J]. Chinese Journal of Endocrinology and Metabolism, 2021, 37(4): 311-398.
[6] Shang A, Li J H, Zhou D D, et al.Molecular mechanisms underlying health benefits of tea compounds[J]. Free Radical Biology and Medicine, 2021, 172: 181-200.
[7] Wan C P, Ouyang J, Li M X, et al.Effects of green tea polyphenol extract and epigallocatechin-3-O-gallate on diabetes mellitus and diabetic complications: recent advances[J]. Critical Reviews in Food Science and Nutrition, 2022: 1-29. doi: 10.1080/10408398.2022.2157372.
[8] Ito A, Yanase E.Study into the chemical changes of tea leaf polyphenols during Japanese black tea processing[J]. Food Research International, 2022, 160: 111731. doi: 10.1016/j.foodres.2022.111731.
[9] Liu Z H, Gao L Z, Chen Z M, et al.Leading progress on genomics, health benefits and utilization of tea resources in China[J]. Nature, 2019, 566(7742): S15-S19.
[10] Xiao Y, Li M Y, Liu Y, et al.The effect of Eurotium cristatum (MF800948) fermentation on the quality of autumn green tea[J]. Food Chemistry, 2021, 358: 129848. doi: 10.1016/j.foodchem.2021.129848.
[11] Portha B.Programmed disorders of β-cell development and function as one cause for type 2 diabetes? The GK rat paradigm[J]. Diabetes/Metabolism Research and Reviews, 2005, 21(6): 495-504.
[12] Zhu J X, Wu M R, Zhou H, et al.Liubao brick tea activates the PI3K-Akt signaling pathway to lower blood glucose, metabolic disorders and insulin resistance via altering the intestinal flora[J]. Food Research International, 2021, 148: 110594. doi: 10.1016/j.foodres.2021.110594.
[13] Nair A B, Jacob S.A simple practice guide for dose conversion between animals and human[J]. Journal of Basic and Clinical Pharmacy, 2016, 7(2): 27-31.
[14] Zhou F, Zhu M Z, Tang J Y, et al.Six types of tea extracts attenuated high-fat diet-induced metabolic syndrome via modulating gut microbiota in rats[J]. Food Research International, 2022, 161: 111788. doi: 10.1016/j.foodres.2022.111788.
[15] 贾建普, 王丽轩, 张乐国, 等. 血清胱抑素C与颈动脉狭窄程度的相关性研究[J]. 中国动脉硬化杂志, 2014, 22(6): 607-612.
Jia J P, Wang L X, Zhang L G, et al.Correlation between serum cystatin C and the degree of carotid stenosis[J]. Chinese Journal of Arteriosclerosis, 2014, 22(6): 607-612.
[16] 王皓晨. 联合测定空腹血糖和糖化血清蛋白在糖尿病检测中的临床疗效分析[J]. 糖尿病新世界, 2021, 24(12): 44-47.
Wang H C.Analysis of clinical efficacy of combined determination of fasting blood glucose and glycosylated serum protein in diabetes detection[J]. Diabetes New World, 2021, 24(12): 44-47.
[17] 张传龙, 李奕, 庞博, 等. 基于《黄帝内经》脾之生理多维度探讨胰腺癌治脾策略[J]. 世界中西医结合杂志, 2023, 18(3): 445-448.
Zhang C L, Li Y, Pang B, et al.Treatment strategy of pancreatic cancer from multiple dimensions of spleen physiology in Huangdi's internal classic (Huang Di Nei Jing)[J]. World Journal of Integrated Traditional and Western Medicine, 2023, 18(3): 445-448.
[18] 童俊, 刘红兵, 刘颖伟, 等. 基于HPLC-ESI-QTOF-MS技术的金桂花降糖活性物质基础研究[J]. 药学学报, 2023, 58(3): 750-759.
Tong J, Liu H B, Liu Y W, et al.Research on hypoglycemic activity of Osmanthus fragrans var. thunbergii extract[J]. Acta Pharmaceutica Sinica, 2023, 58(3): 750-759.
[19] 曾鸿哲, 周方, 刘昌伟, 等. 茶及其功能成分对肠道菌群调节作用的研究进展[J]. 中国茶叶加工, 2022(1): 5-10.
Zeng H Z, Zhou F, Liu C W.Research progress on the regulatory effect of tea and its functional components on intestinal flora[J]. China Tea Processing, 2022(1): 5-10.
[20] 王旭红, 史捷, 张暮盈, 等. 基于“肺肠同治”的清肺化痰逐瘀汤治疗COPD的作用机制[J/OL]. 中国实验方剂学杂志, 2023: 1-12[2023-07-05]. doi: 10.13422/j.cnki.syfjx.20231107.
Wang X H, Shi J, Zhang M Y. Mechanism of Qingfei Huatan Zhuyu Decoction in treatment of COPD based on "Lung and Intestinal Syntherapy"[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2023: 1-12[2023-07-05]. doi: 10.13422/j.cnki.syfjx.20231107.
[21] 舒毅, 钟历勇, 石立. 褪黑素、维生素E和还原型谷胱甘肽对四氧嘧啶糖尿病大鼠氧化应激影响的比较研究[J]. 东南大学学报(医学版), 2006, 25(2): 88-92.
Shu Y, Zhong L Y, Shi L, et al.Comparative analysis of effects of melatonin, vitamin E and glutathione on oxidative stress in alloxan-induced diabetic rats[J]. Journal of Southeast University (Medical Science Edition), 2006, 25(2): 88-92.
[22] Newsome P N, Cramb R, Davison S M, et al.Guidelines on the management of abnormal liver blood tests[J]. Gut, 2018, 67(1): 6-19.
[23] Taylor S R, Ramsamooj S, Liang R J, et al.Dietary fructose improves intestinal cell survival and nutrient absorption[J]. Nature, 2021, 597(7875): 263-267.
[24] Al-Mrabeh A, Zhyzhneuskaya S V, Peters C, et al.Hepatic lipoprotein export and remission of human type 2 diabetes after weight loss[J]. Cell Metabolism, 2020, 31(2): 233-249
[25] 柳剑, 蓝绍颖. 2型糖尿病危险因素的流行病学研究进展[J]. 南通大学学报(医学版), 2006, 26(3): 230-232.
Liu J, Lan S Y.Epidemiologic review of risk factors for type 2 diabetes mellitus[J]. Journal of Nantong University (Medical Sciences), 2006, 26(3): 230-232.
[26] Huang F J, Zheng X J, Ma X H, et al.Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism[J]. Nature Communications, 2019, 10(1): 4971. doi: 10.1038/s41467-019-12896-x.
[27] Yue S J, Shan B, Peng C X, et al.Theabrownin-targeted regulation of intestinal microorganisms to improve glucose and lipid metabolism in Goto-Kakizaki rats[J]. Food & Function, 2022, 13(4): 1921-1940.
[28] Yang W Q, Ren D Y, Shao H J, et al.Theabrownin from Fu brick tea improves ulcerative colitis by shaping the gut microbiota and modulating the tryptophan metabolism[J]. Journal of Agricultural and Food Chemistry, 2023, 71(6): 2898-2913.
[29] Petersen M C, Shulman G I.Mechanisms of insulin action and insulin resistance[J]. Physiological Reviews, 2018, 98(4): 2133-2223. doi: 10.1152/physrev.00063.2017.
[30] 何勇, 彭家志, 李家明, 等. 基于PPARs为靶点的抗糖尿病药物研究进展[J]. 亚太传统医药, 2009, 5(3): 129-131.
He Y, Peng J Z, Li J M, et al.Research progress of anti-diabetic drugs based on PPARs as targets[J]. Asia-Pacific Traditional Medicine, 2009, 5(3): 129-131.
[31] 魏爽, 李冀, 韩东卫, 等. 基于网络药理学探讨黄芪-葛根药对对T2DM模型大鼠PI3K/Akt/FoxO1信号通路的调节作用[J]. 中国实验方剂学杂志, 2022, 28(5): 157-168.
Wei S, Li Y, Han D W, et al.Regulatory effect of herbal pair astragali radix-puerariae lobatae radix on P13K/Akt/FoxO1 signaling pathway in rats with T2DM based on network pharmacology[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(5): 157-168.