By the experimental procedure of enrichment culture and microbial purification, 6 azotobacter strains were screened from the soil of tea garden in Shandong province. According to the activity comparison and taxonomy of strains, two different strains were screened. The strain GD 5 was preliminary identified as Azotobacter chrococcum, GD15 as Pseudomonas putida, on the basis of strain′s morphological characteristics, physiological and biochemical characteristics and 16 S rDNA sequencing method. The result showed that the optimum pH for GD5, GD15 is 7, the optimum temperature of two strains is 30℃. The GD5 showed the phosphate solubilizing power and auxin production ability, and the GD15 showed the auxin production activity only. Compared with the control, the dry weight of root, stem, leaf of tea plant inoculated with GD5 strain increased by 42%, 58%, 55% respectively. The nitrogen content of shoot and root of tea plant inoculated with GD5 strain increased 15% and 6%. It showed that the GD5 strain possessed a good developmental prospect.
HAN Xiaoyang
,
LI Zhi
,
ZHANG Lixia
,
HUANG Xiaoqin
. Screening, Identification and Inoculation Effect of Azotobacter from the Soils of Tea Garden[J]. Journal of Tea Science, 2014
, 34(5)
: 497
-505
.
DOI: 10.13305/j.cnki.jts.2014.05.011
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