Screening, Identification and Inoculation Effect of Azotobacter from the Soils of Tea Garden

HAN Xiaoyang; LI Zhi; ZHANG Lixia; HUANG Xiaoqin

doi:10.13305/j.cnki.jts.2014.05.011

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (5) : 497-505. DOI: 10.13305/j.cnki.jts.2014.05.011

Screening, Identification and Inoculation Effect of Azotobacter from the Soils of Tea Garden

HAN Xiaoyang, LI Zhi, ZHANG Lixia^*, HUANG Xiaoqin

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Abstract

By the experimental procedure of enrichment culture and microbial purification, 6 azotobacter strains were screened from the soil of tea garden in Shandong province. According to the activity comparison and taxonomy of strains, two different strains were screened. The strain GD 5 was preliminary identified as Azotobacter chrococcum, GD15 as Pseudomonas putida, on the basis of strain′s morphological characteristics, physiological and biochemical characteristics and 16 S rDNA sequencing method. The result showed that the optimum pH for GD5, GD15 is 7, the optimum temperature of two strains is 30℃. The GD5 showed the phosphate solubilizing power and auxin production ability, and the GD15 showed the auxin production activity only. Compared with the control, the dry weight of root, stem, leaf of tea plant inoculated with GD5 strain increased by 42%, 58%, 55% respectively. The nitrogen content of shoot and root of tea plant inoculated with GD5 strain increased 15% and 6%. It showed that the GD5 strain possessed a good developmental prospect.

Key words

azotobacter / identification / inoculation / tea garden soil

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HAN Xiaoyang, LI Zhi, ZHANG Lixia, HUANG Xiaoqin. Screening, Identification and Inoculation Effect of Azotobacter from the Soils of Tea Garden[J]. Journal of Tea Science. 2014, 34(5): 497-505 https://doi.org/10.13305/j.cnki.jts.2014.05.011

References

[1] 孙建光, 张燕春, 徐晶, 等. 玉米根际高效固氮菌 Sphingomonas sp. GD542的分离鉴定及接种效果初步研究[J]. 中国生态农业学报, 2010, 18(1): 89-93.
[2] 姚拓, 龙瑞军, 王刚, 等. 兰州地区盐碱地小麦根际联合固氮菌分离及部分特性研究[J]. 2004, 41(3): 444-448.
[3] 席琳乔, 李德锋, 王静芳, 等. 棉花根际促生菌固氮和分泌生长激素能力的测定[J]. 干旱区研究, 2008, 25(5): 690-694.
[4] 陈文新. 生物固氮[C]//中国土壤学会. 氮素循环与农业和环境学术研讨会论文集. 厦门: 厦门大学出版社, 2001: 4-5.
[5] Bae H S, Rash A, Fred A, et al. Description of Azospira restricta sp. nov., a nitrogen-fixing bacterium isolated from groundwater[J]. Int J Syst Evol Microbiol, 2007, 57: 1521-1526.
[6] Mehnaz S, Weselowski B, Lazarovits G.Azospirillum canadense sp nov, a nitrogen-fixing bacterium isolated from corn rhizosphere[J]. Int J Syst Evol Microbiol, 2007, 57: 620-624.
[7] 田永辉. 不同树龄茶树根际固氮菌组成及多样性研究[J]. 福建茶叶, 2000(3): 19-23.
[8] 田永辉. 不同土壤对茶树根际固氮微生物的影响[J]. 茶叶通讯, 1999(4): 22-23.
[9] 赵斌, 何绍江. 微生物学实验[M]. 北京: 科学出版社, 2002: 215
[10] 田宏, 张德罡, 姚拓, 等. 禾本科草坪草固氮菌株筛选及部特性初步研究[J]. 中国草地, 2005, 2(5): 47-52.
[11] Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227: 680-685.
[12] 谭志远, 陈文新. 根瘤菌新类群的全细胞蛋白电泳及16S rDNA全序列分析[J]. 应用与环境生物学报, 1998, 4(1): 65-69.
[13] Tan Z Y, Kan F L, Peng G X, et al. Rhizobium yanglingense sp nov, isolated from arid and semi-arid regions in China[J]. Int J Syst Evol Microbiol, 2001, 51: 909-914.
[14] Weisburgw G, Bams M, Elletier D A.16sRibosomal DNA Amplification for Phylogenetic Study[J]. J Bacterial, 1991, 173(2): 697-703.
[15] Martin Könneke, Anne E Bernhard, José R de la Torre, et al. Isolation of an autotrophic ammonia-oxidizing marine archaeon[J]. Nature, 2005, 437: 543-546.
[16] Buchanan R E, Gibbons N E.伯杰细菌鉴定手册[M]. 北京: 科学出版社, 1984: 329-339.
[17] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 138-140.
[18] Eric Glickmann, Yves Dessaux.A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J]. Applied and Environmental Microbiology, 1995(2): 793-796.
[19] 叶劲松, 吴克, 俞志敏. 1 株无机磷细菌筛选及溶磷能力的测定[J]. 江苏农业科学, 2013, 41(6): 333-335.
[20] 许光辉, 郑洪元. 土壤微生物分析方法手册[M]. 北京: 农业出版社, 1986: 246-248.
[21] Rosch C, Mergel A, Bothe H.Biodiversity of denitrifying and dinitrogen_fixing bacteria in an acid forest soil[J]. Appl Environ Microbiol, 2002, 68: 3818-3829.
[22] 黄晓琴, 束怀瑞, 刘会香, 等. 山东省茶树冰核细菌的鉴定[J]. 茶叶科学, 2010, 30(3): 191-194.
[23] 尹瑞龄. 我国旱地土壤的溶磷微生物[J]. 土壤, 1988, 20(5): 243-246.
[24] 姚拓. 高寒地区燕麦根际联合固氮菌研究[J]. 草业学报, 2004, 13(3): 85-90.
[25] 张晓勇, 刘义平. 玉米联合固氮菌的溶磷作用[J]. 新疆农业科学, 1992, 14(6): 264-265.
[26] Malik K A, RasulG, Hassan U, et al. Role of N₂-fixing and growth hormones producing bacteria in improving growth of wheat and rice[C]//Hegazi NA. Proceedings of 6th Int Symposium on Nitrogen Fixation with Non-legumes. Egypt: Ismailia, 1994: 409-422.
[27] Kobus J.The distribution of microorganisms mobilizing phosphorus in different soil[J]. Acta Microbiologia Plolonica, 1962, 11: 255-264.
[28] 秦芳玲, 王敬国, 李晓林, 等. VA菌根真菌和解磷细菌对红三叶草生长和氮磷营养的影响[J]. 草业学报. 2000, 9(1): 9-14.
[29] 林启美, 赵小蓉, 孙焱鑫, 等. 四种不同生态系统的土壤解磷细菌数量及种群分布[J]. 土壤与环境, 2000, 9(1): 34-37.
[30] Kucey R M.Phosphate-solubilizing bacteria and fungi in various cultivated and virgin Alberta soils[J]. Canadian Journal of Soil Science, 1983, 63: 671-678.
[31] 姚拓, 蒲小鹏, 张德罡, 等. 高寒地区燕麦根际联合固氮菌研究Ⅲ固氮菌对燕麦生长的影响及其固氮量测定[J]. 草业学报, 2004, 13(1): 101-110.
[32] 曹云海. 非豆科作物施用固氮菌肥的肥效试验[J]. 甘肃农业, 2005(11): 93-91.
[33] 林代炎, 叶美锋, 林琰, 等. ¹⁵N示踪法研究固氮芽孢杆菌应用效果[J]. 生态环境, 2006, 15(6): 1310-1312.
[34] 施振云, 王德君. 联合固氮菌剂拌种对玉米苗期生长和产量的影响[J]. 玉米科学, 2000, 8(1): 69-71.
[35] 方萍, 张丽梅, 贾小明, 等. 固氮螺菌(Azosprillumbrasilense)NO40在红壤性水稻土上的接种效应[J]. 浙江大学学报: 自然科学版, 2001, 27(1): 33-36.