In order to compare the effect of Dahongpao tea water extract feeding at different time on alcohol-induced acute liver injury in mice. Fifty mice were randomly divided into five groups and were intragastrically given 60% ethanol (0.01βmL/g) alcohol (alcohol group) or distilled water (normal control). Dahongpao tea water extract was fed at different time, including feeding 2 hours before drinking, feeding while drinking and feeding 2 hours after drinking alcohol. All mice were sacrificed to take serum samples for biochemical measurements, such as glutamic oxalacetic transaminase (AST), glutamic-pyruvic transminase (ALT), total cholesterol (TC), total triglyceride (TG), malondialdehyde (MDA) and total sulfydryl, liver samples for microexamination using HE staining to determine the degree of fatty changes. A mice model of alcohol induced acute liver injury has been successfully established biochemical metabolism index, antioxidant index and microexamination results in mice liver showed that Dahongpao tea water extract fed at different time is able to protect mice from alcohol-induced acute liver injury in different degree. Fed 2 hours before drinking is of best protective effect, and fed while drinking is the second, However, the effect of fed 2 hours after drinking is not significant.