In order to explore extra-cellular enzyme secreted by Eurotium cristatum fungi, the enzymatic productivity and activity changes on carboxymethyl cellulase(CMCase), proteinase, amylase, pectinase and lipase were analyzed according to the methods of hydrolyzed circle and liquid cultivation. The results showed that there were obvious hydrolyzed circles appearsed on the plate medium with starch and olive oil, but not on plate medium with sodium carboxymethyl cellulose, pectin and milk. Except lipase, other enzymes were detected and their activities were varied obviously during liquid cultivation.
CHEN Gui-mei
,
DENG Yong-liang
,
HUANG Ya-ya
,
ZHANG Yue
,
JI Xiao-ming
,
ZHOU Xing-chang
,
HU Xin
. Activity Changes in Several Extracellular Enzymes of Eurotium cristatum Fungi During its Growth Period[J]. Journal of Tea Science, 2013
, 33(4)
: 306
-310
.
DOI: 10.13305/j.cnki.jts.2013.04.003
[1] 齐祖同, 孙曾美. 茯砖茶中优势菌种的鉴定[J]. 真菌学报, 1990, 9(3): 176-179.
[2] 温琼英, 刘素纯. 茯砖茶发花中优势菌的演变规律[J]. 茶叶科学, 1991, 11(增刊1): 56-62.
[3] 蔡正安, 刘素纯, 刘仲华, 等. 茯砖茶中冠突散囊菌纤维素酶的酶学性质研究[J]. 茶叶科学, 2010, 30(1): 57-62.
[4] 刘巧林. 液态发酵茶工艺参数及相关酶活性的研究[D]. 成都: 四川农业大学, 2007.
[5] 邓放明. 茯砖茶中冠突散囊菌分离培养及其发酵液胞外多糖与应用酶学研究[D]. 长沙: 湖南农业大学, 2007.
[6] 蔡俊鹏, 陈华, 林阿乞. 福建大连1号杂交鲍肠道及养殖水体中细菌产酶能力的分析[J]. 水产科学, 2008, 27(3): 129-134.
[7] 钦传光, 丹·娃伦亭娜, 诺·狄安娜. 果胶酶高产菌种的筛选[J]. 中国酿造, 2000(4): 14-18.
[8] 李乃强, 潘军华, 张星元. 霉菌酸性蛋白酶高产突变菌株9169的选育[J]. 无锡轻工大学学报, 2001(9): 493-496.
[9] 乔红群, 徐虹, 付闪雷, 等. 脂肪酶产生菌的筛选及其酶性质[J]. 南京化工大学学报, 1998(1): 15-19.
[10] 姚茂君, 黄群, 陈林杰. 冠突散囊菌的分离及其液态发酵特性[J]. 食品与发酵工业, 2007, 33(6): 28-31.
[11] 何红霞. “散茶发花”工艺微生物类群及其对茶叶品质形成影响的研究[D]. 长沙: 湖南农业大学, 2012.
[12] 王增盛, 施兆鹏, 刘仲华, 等. 论茯砖茶品质风味形成机理[J]. 茶叶科学, 1991, 11(增刊1): 49-55.
[13] 王若仲. 纤维素酶、果胶酶在茶叶加工中的研究现状[J]. 贵州茶叶, 1998(2): 21.
[14] 陈椽. 制茶技术理论[M]. 上海: 上海科学技术出版社, 1984: 10-57.
[15] 丁婷, 吕嘉枥, 侯蓓. 茯砖茶中“金花”菌产蛋白酶特性的研究[J]. 陕西科技大学学报, 2011, 29(6): 13-20.
[16] 陆建良. 茶汤蛋白对茶饮料冷后浑形成的影响[D]. 杭州: 浙江大学, 2008.