With C18 bonded silica as stationary phase, ethanol and water (20︰80, V/V) as mobile phase, tea polyphenols were separated by traditional Simulated Moving Bed, Varicol process and Partial-discard process. Column configuration, switching time, feed flow rate, elution flow rate were optimized respectively in order to obtain epicatechin gallate (ECG) and epigallocatechin gallate(EGCG)with high purity and high recovery. Varicol process could be employed to separate 98% green tea polyphenols (TP98) to obtain ECG with 91.33% purity and 91.41% recovery. Partial-discard process could be adopted to separate TP98 to obtain EGCG with 90.12% purity and 97.83% recovery. Compared with traditional process, Varicol process has the advantages of higher purity and recovery of target product, while Partial-discard process shows the advantages of increasing the concentration of raffinate and the purity of target product.
HUANG Yong-dong
,
JIANG He-yuan
,
JIANG Yong-wen
,
ZHANG Jian-yong
,
WANG Bin
,
BAI Yan
,
WEN Zhi-hao
,
WANG Wei-wei
. Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography[J]. Journal of Tea Science, 2011
, 31(3)
: 201
-210
.
DOI: 10.13305/j.cnki.jts.2011.03.007
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