Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

HUANG Yong-dong; JIANG He-yuan; JIANG Yong-wen; ZHANG Jian-yong; WANG Bin; BAI Yan; WEN Zhi-hao; WANG Wei-wei

doi:10.13305/j.cnki.jts.2011.03.007

PDF(460 KB)

Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (3) : 201-210. DOI: 10.13305/j.cnki.jts.2011.03.007

Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography

HUANG Yong-dong^1,2,3, JIANG He-yuan^1,2*, JIANG Yong-wen^1,2, ZHANG Jian-yong^1,2, WANG Bin^1,2,3, BAI Yan^1,2,3, WEN Zhi-hao^1,2,3, WANG Wei-wei^1,2,3

Author information +

History +

Abstract

With C₁₈ bonded silica as stationary phase, ethanol and water (20︰80, V/V) as mobile phase, tea polyphenols were separated by traditional Simulated Moving Bed, Varicol process and Partial-discard process. Column configuration, switching time, feed flow rate, elution flow rate were optimized respectively in order to obtain epicatechin gallate (ECG) and epigallocatechin gallate(EGCG)with high purity and high recovery. Varicol process could be employed to separate 98% green tea polyphenols (TP98) to obtain ECG with 91.33% purity and 91.41% recovery. Partial-discard process could be adopted to separate TP98 to obtain EGCG with 90.12% purity and 97.83% recovery. Compared with traditional process, Varicol process has the advantages of higher purity and recovery of target product, while Partial-discard process shows the advantages of increasing the concentration of raffinate and the purity of target product.

Key words

ECG / EGCG / partial-discard / simulated moving bed / varicol

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

HUANG Yong-dong, JIANG He-yuan, JIANG Yong-wen, ZHANG Jian-yong, WANG Bin, BAI Yan, WEN Zhi-hao, WANG Wei-wei. Comparsion of Varicol and Partial-discard Process with Traditional process to Purify ECG and EGCG from Green Tea Polyphenols Using Simulated Moving Bed Chromatography[J]. Journal of Tea Science. 2011, 31(3): 201-210 https://doi.org/10.13305/j.cnki.jts.2011.03.007

References

[1] 林炳昌. 模拟移动床色谱技术[M]. 北京: 化学工业出版社, 2008: 1.
[2] Broughton D B, Gerhold C G. Continuous sorption process employing fixed bed of sorbent and moving inlets and outlets: US, 2985589[P].1961-5-23.
[3] Universal Oil Products Co. Understanding the SorbexTM Process [EB/OL]. [2010-08-02]. http://www.uop.com/.
[4] Long N V D, Le T H, Kim J I, et al. Separation of D-psicose and D-fructose using simulated moving bed chromatography[J]. Journal of Separation Science, 2009, 32(11): 1987-1995.
[5] Makart S, Bechtold M, Panke S.Separation of amino acids by simulated moving bed under solvent constrained conditions for the integration of continuous chromatography and biotransformation[J]. Chemical Engineering Science, 2008, 63(21): 5347-5355.
[6] Rajendranb A, Paredesa G, Mazzotti M.Simulated moving bed chromatography for the separation of enantiomers[J]. Journal of Chromatography A, 2009, 1216(4): 709-738.
[7] Juza M, Mazzotti M, Morbidelli M.Simulated moving-bed chromatography and its application to chirotechnology[J]. Trends in Biotechnology, 2000, 18(3): 108-118.
[8] Kaiser P, Ottolina G, Carrea G, et al. Preparative-scale separation by simulated moving bed chromatography of biocatalytically produced regioisomeric lactones[J]. New Biotechnology, 2009, 25(4): 220-225.
[9] Wei F, Zhao Y X.Separation of capsaicin from capsaicinoids by simulated moving bed chromatography[J]. Journal of Chromatography A, 2008, 1187(1/2): 281-284.
[10] 赵雪飞, 高丽娟, 赖仕全. 模拟移动床色谱法分离C60的条件选择[J]. 辽宁科技大学学报, 2008, 31(3/4): 225-228.
[11] Olivier L H, Marc N R. Process and device for separation with variable-length chromatographic zones: US, 6712973[P].2004-03-30.
[12] Zhang Z Y, Mazzotti M, Morbidelli M.PowerFeed operation of simulated moving bed units: changing flow-rates during the switching interval[J]. Journal of Chromatography A, 2003, 1006(1/2): 87-99.
[13] Kawajiri Y, Biegler L T.Optimization strategies for simulated moving bed and PowerFeed processes[J]. American Institute of Chemical Engineers Journal, 2007, 52(4): 1343-1350.
[14] Schramm H, Kaspereit M, Kienle A, et al. Simulated moving bed process with cyclic modulation of the feed concentration[J]. Journal of Chromatography A. 2003, 1006(1/2): 77-86.
[15] Schramm H, Kaspereit M, Kienle A, et al. Improving simulated moving bed processes by cyclic modulation of the feed concentration[J]. Chem Eng Technol, 2002, 25(12): 1151-1155.
[16] Keßler L C, Seidel-Morgenstern A.Improving performance of simulated moving bed chromatography by fractionation and feed-back of outlet streams[J]. Journal of Chromatography A, 2008, 1207(1/2): 55-71.
[17] Migliorini C, Wendlinger M, Mazzotti M.Temperature gradient operation of a simulated moving bed unit[J]. Ind Eng Chem Res, 2001, 40(12): 2606-2617.
[18] Mun S Y, Wang N H L. Optimization of productivity in solvent gradient simulated moving bed for paclitaxel purification[J]. Process Biochemistry, 2008, 43(12): 1407-1418.
[19] Mun S Y.Enhanced Separation Performance of a Five-Zone Simulated Moving Bed Process by Using Partial Collection Strategy Based on Alternate Opening and Closing of a Product Port[J]. Industrial & Engineering Chemistry Research, 2010, 49(19): 9258-9270.
[20] Paredes G, Abel S, Mazzotti M, et al. Analysis of a Simulated Moving Bed Operation for Three-Fraction Separations (3F-SMB)[J]. Ind Eng Chem Res, 2004, 43(19): 6157-6167.
[21] 钟世安, 周春山, 杨娟玉. 高效液相色谱法分离纯化酯型儿茶素的研究[J]. 化学世界, 2003, 44(5): 237-239, 245, 249.
[22] 张莹, 施兆鹏, 聂洪勇, 等. 制备型逆流色谱分离绿茶提取物中儿茶素单体[J]. 湖南农业大学学报, 2003, 29(5): 408-411, 417.
[23] 姜绍通, 黄静, 潘丽军, 等. 儿茶素单体的分离纯化方法: 中国, 1603319[P], 2005-04-06.
[24] 蔡宇杰, 丁彦蕊, 张大兵, 等. 模拟移动床色谱技术及其应用[J]. 色谱, 2004, 22(2): 111-115.
[25] 张家元, 林炳昌. 三带模拟移动床色谱的实验研究[J]. 鞍山钢铁学院学报, 1999(3): 144-148.
[26] Ludemann-Hombourger O, Nicoud, R M, Bailly M. The Varicol Process: A New Multicolumn Continuous Chromatographic Process[J]. Sep Sci Technol, 2000, 35(12): 1829-1862.
[27] Zhang Z Y, Hidajat K, Ray A K, et al. Multiobjective Optimization of SMB and Varicol Process for Chiral Separation[J]. AIChE Journal, 2002, 48(12): 2800-2816.
[28] Toumi A, Engell S, Ludemann-Hombourger O, et al. Optimization of simulated moving bed and Varicol processes[J]. Journal of Chromatography A, 2003, 1006(1/2): 15-31.
[29] Zhang Z Y, Mazzotti M, Morbidelli M.Continuous Chromatographic Processes with a Small Number of Columns: Comparison of Simulated Moving Bed with Varicol, PowerFeed, and ModiCon[J]. Korean J Chem Eng, 2004, 21(2): 454-464.
[30] Zhang Y, Hidajat K, Ray A K.Multi-objective optimization of simulated moving bed and Varicol processes for enantio-separation of racemic pindolol[J]. Separation and Purification Technology, 2009, 65(3): 311-321.
[31] Bae Y S, Lee C H.Partial-discard strategy for obtaining high purity products using simulated moving bed chromatography[J]. Journal of Chromatography A, 2006, 1122(1/2): 161-173.
[32] Keßler L C, Seidel-Morgenstern A.Improving performance of simulated moving bed chromatography by fractionation and feed-back of outlet streams[J]. Journal of Chromatography A, 2008, 1207(1/2): 55-71.
[33] Ludemann-hombourger Olivier, Nicoud Roger Marc. Process and device for separation with variable-length chromatographic zones: US, 6712973[P].2004-03-30.
[34] Yan Zhang, Kus Hidajat, Ajay K Ray.Enantio-separation of racemic pindolol on α1-acid glycoprotein chiral stationary phase by SMB and Varicol[J]. Chemical Engineering Science. 2007, 62(5): 1364-1375.