It was of much importance using minimum primers to obtain the maximum identification ability．Molecular identification of 134 tea germplasm resources using inter-simple sequence repeat (ISSR) markers was conducted in this study. There were 3 independent ways to identify tea germplasms: a) unique ISSR markers;b) unique band patterns and c) a combination of the band patterns provided by different primers．The result showed that the presence of 10 unique ISSR markers and the absence of 15 unique markers obtained from 12 primers made it possible to identify 21 tea germplasms, including ‘Xiangzhuqingdashancha’ and so on. Using 54 unique band patterns of primer UBC811 could identify 35 tea germplasms, including ‘Hainandayecha 1’ and so on. A1l the 134 tea germplasms could be entirely identified by the band patterns combination of primer UBC811, UBC835, ISSR2 and ISSR3, which was successfully used to construct the ISSR fingerprinting for discriminating 134 tea germplasms from Yunnan Province.
LIU Ben-ying
,
WANG Li-yuan
,
LI You-yong
,
TANG Yi-chun
,
HE Wei
,
CHENG Hao
,
WANG Ping-sheng
. ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province[J]. Journal of Tea Science, 2009
, 29(5)
: 355
-364
.
DOI: 10.13305/j.cnki.jts.2009.5.005
[1] Zietkiewicz E, Rafalski A, Labuda D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20: 176~183.
[2] Reddy MP, Sarla N, Siddiq EA.Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding[J]. Euphytica, 2002, 128: 9~17.
[3] 陈亮, 王平盛, 山口聪. 应用RAPD分子标记鉴定野生茶树种质资源研究[J]. 中国农业科学, 2002, 35(10): 1186~1191.
[4] 陈亮, 杨亚军, 虞富莲. 应用RAPD标记进行茶树优异种质资源遗传多态性、亲缘关系分析与分子鉴别[J]. 分子植物育种, 2004, 2(3): 385~390.
[5] Chen L, Gao QK, Chen DM, et al. The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in tea germplasm repository[J]. Biodiversity and Conservation, 2005, 14(6): 1433~1444.
[6] 黎星辉, 施兆鹏, 刘春林, 等. 云南大叶茶与汝城白毛茶杂交后代的RAPD鉴定[J]. 茶叶科学, 2001, 21(2): 99~102.
[7] Zietkiewicz E, Rafalski A, Labuda D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20: 176~183.
[8] Reddy MP, Sarla N, Siddiq EA.Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding[J]. Euphytica, 2002, 128: 9~17.
[9] 陈亮, 王平盛, 山口聪. 应用RAPD分子标记鉴定野生茶树种质资源研究[J]. 中国农业科学, 2002, 35(10): 1186~1191.
[10] 陈亮, 杨亚军, 虞富莲. 应用RAPD标记进行茶树优异种质资源遗传多态性、亲缘关系分析与分子鉴别[J]. 分子植物育种, 2004, 2(3): 385~390.
[11] Chen L, Gao QK, Chen DM, et al. The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in tea germplasm repository[J]. Biodiversity and Conservation, 2005, 14(6): 1433~1444.
[12] 黎星辉, 施兆鹏, 刘春林, 等. 云南大叶茶与汝城白毛茶杂交后代的RAPD鉴定[J]. 茶叶科学, 2001, 21(2): 99~102.
[13] 梁月荣, 田中淳一, 武田善行. 应用RAPD分子标记分析“晚绿”品种的杂交亲本[J]. 茶叶科学, 2000, 20(1): 22~26.
[14] Yang W, de Oliveira A C, Godwin I, et al. Comparison of DNA marker technologies in characterizing plant genome diversity:variability in Chinese sorghums[J]. Crop Science, 1996, 36: 1669~1676.
[15] 刘本英, 王平盛, 周红杰, 等. 云南茶组植物ISSR-PCR反应体系的建立[J]. 云南农业大学学报, 2006(增): 21~25.
[16] 刘本英, 王丽鸳, 周健等. 云南大理茶与福鼎大白茶种间杂交幼胚的组织培养及亲子鉴定[J]. 园艺学报, 2008, 35(5): 735~740.
[17] 王凤格, 赵久然, 郭景伦, 等. 比较三种DNA指纹分析方法在玉米品种纯度及真伪鉴定中的应用[J]. 分子植物育种, 2003, 1(5): 655~662.
[18] 柳李旺, 候喜林, 龚义勤, 等. 分子标记技术在蔬菜作物品种鉴定与纯度检测中的应用.分子植物育种[J]. 2004, 2(4): 563~568.
[19] Lee SH, Choi HS, Kim RH, et al. Identification of Korean wild tea plants and Japanese green tea germplasms using RAPD markers[J]. J Kor Tea Soc, 1995, 1(1): 129~148.
[20] Wachira FN, Powell W, Waugh R.An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle-specific STS[J]. Heredity, 1997,78: 603~611.
[21] 陈亮, 杨亚军, 虞富莲, 等. 15个茶树品种遗传多样性的RAPD分析[J]. 茶叶科学, 1998, 18(1): 21~27.
[22] Paul S, Wachira FN, Powell W, et al. Polymorphism and genetic differentiation among populations of Indian and Kenyan tea [Camellia sinensis (L.) O. Kuntze] revealed by AFLP markers[J]. Theor Appl Genet, 1997, 94: 255~263.
[23] Chen L, Yamaguchi S.RAPD markers for discriminating tea germplasms on the inter-specific level in China[J]. Plant Breeding, 2005, 124: 404~409.
[24] Wachira FN, Tanaka J, Takeda Y.Genetic variation and differentiation in tea (Camellia sinensis) germplasm revealed by RAPD and AFLP variation[J]. J Hort Sci & Biotech, 2001,76(5): 557~563.
[25] 黄福平, 梁月荣, 陆建良, 等. 乌龙茶种质资源种群遗传多样性AFLP评价[J]. 茶叶科学, 2004, 24(3): 183~189.
[26] Matsumoto S, Kiriiwa Y, Takeda Y.Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia-lyase DNA[J]. Theor Appl Genet, 2002: 104: 998~1002.
[27] 姚明哲, 陈亮, 王新超, 等. 我国茶树无性系品种遗传多样性和亲缘关系的ISSR分析[J]. 作物学报, 2007, 33(4): 598~604.
浙公网安备 33019902000101号