ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province

LIU Ben-ying; WANG Li-yuan; LI You-yong; TANG Yi-chun; HE Wei; CHENG Hao; WANG Ping-sheng

doi:10.13305/j.cnki.jts.2009.5.005

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5) : 355-364. DOI: 10.13305/j.cnki.jts.2009.5.005

ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province

LIU Ben-ying^1,2, WANG Li-yuan¹, LI You-yong², TANG Yi-chun², HE Wei¹, CHENG Hao^1,*, WANG Ping-sheng²

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Abstract

It was of much importance using minimum primers to obtain the maximum identification ability．Molecular identification of 134 tea germplasm resources using inter-simple sequence repeat (ISSR) markers was conducted in this study. There were 3 independent ways to identify tea germplasms: a) unique ISSR markers;b) unique band patterns and c) a combination of the band patterns provided by different primers．The result showed that the presence of 10 unique ISSR markers and the absence of 15 unique markers obtained from 12 primers made it possible to identify 21 tea germplasms, including ‘Xiangzhuqingdashancha’ and so on. Using 54 unique band patterns of primer UBC811 could identify 35 tea germplasms, including ‘Hainandayecha 1’ and so on. A1l the 134 tea germplasms could be entirely identified by the band patterns combination of primer UBC811, UBC835, ISSR2 and ISSR3, which was successfully used to construct the ISSR fingerprinting for discriminating 134 tea germplasms from Yunnan Province.

Key words

identification / ISSR / tea germplasms

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LIU Ben-ying, WANG Li-yuan, LI You-yong, TANG Yi-chun, HE Wei, CHENG Hao, WANG Ping-sheng. ISSR Markers for Discriminating Tea Germplasm Resources from Yunnan Province[J]. Journal of Tea Science. 2009, 29(5): 355-364 https://doi.org/10.13305/j.cnki.jts.2009.5.005

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