Expression conditions of the genetic engineering E.coli which expressed E.coli γ-GGT were investigated. The optimum IPTG concentration and inductive temperature were determined. Factorial experiment design was employed to determine the effects of the reactive conditions (such as microbe concentration, pH, substitute concentration, reactive temperature) on theanine biosynthesis. Results showed that theanine biosynthesis was inhibited by the high microbe concentration. By the higher concentration of L-Gln, the more theanine was obtained and the convertion rate against Gln was declined. It also was manifested that pH9.5 and 32℃~37℃ were optimum conditions for theanine biosynthsis.
WANG Li-yuan
,
WANG Xian-bo
,
CHENG Hao
,
ZHOU Jian
. Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli[J]. Journal of Tea Science, 2007
, 27(2)
: 111
-116
.
DOI: 10.13305/j.cnki.jts.2007.02.003
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