基因工程菌生物合成茶氨酸条件研究

doi:10.13305/j.cnki.jts.2007.02.003

Abstract

Abstract: Expression conditions of the genetic engineering E.coli which expressed E.coli γ-GGT were investigated. The optimum IPTG concentration and inductive temperature were determined. Factorial experiment design was employed to determine the effects of the reactive conditions (such as microbe concentration, pH, substitute concentration, reactive temperature) on theanine biosynthesis. Results showed that theanine biosynthesis was inhibited by the high microbe concentration. By the higher concentration of L-Gln, the more theanine was obtained and the convertion rate against Gln was declined. It also was manifested that pH9.5 and 32℃~37℃ were optimum conditions for theanine biosynthsis.

Key words: theanine, genetically engineered bacteria, expression, biosynthesis

CLC Number:

S571.1
Q523

WANG Li-yuan, WANG Xian-bo, CHENG Hao, ZHOU Jian. Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli[J]. Journal of Tea Science, 2007, 27(2): 111-116.

References 15

[1]	Kakuda T, Nozawa A, Unno T, et al. Inhibiting effects of theanine on caffeine stimulation evaluated by EEG in the rat[J]. Biosci Biotechnol Biochem, 2000, 64(2): 287~293.
[2]	Terashima T, Takido J, Yokogoshi H.Time-dependent changes of amino acids in the serum, liver, brain and urine of rats adminnistered with theanine[J]. Biosci Biotech Biochem, 1999, 63(4): 615~618.
[3]	Yokogoshi H, Kobayashi M, Mochizuki M, et al. Effect of Theanine, r-Glutamylethylamide, on Brain Monoamines and Striatal Dopamine Release in Conscious Rats[J]. Neurochemical Research, 1998, 23(5): 667~673.
[4]	Yokogoshi H, Kobayashi M.Hypotensive effect of gamma-glutamethylamide in spontaneously hypertensive rats[J]. Life Sci, 1998, 62(12): 1065~1068.
[5]	Yokogoshi H, Mochizuki M, Saitoh K.Reduction effect of theanine of brain serotonin concentration in rats[J]. Biosci Biotechnol Biochem, 1998, 62(4): 816~817.
[6]	林雪玲, 程朝辉, 黄才欢, 等. 茶氨酸对小鼠学习记忆能力的影响[J]. 食品科学, 2004, 25(5): 171~173.
[7]	杨建华, 石一复. 茶氨酸抗肿瘤作用及其机制研究进展[J]. 国外医学: 肿瘤学分册, 2004, 31(9): 686~689.
[8]	Sugiyama T, Sadzuka Y.Theanine and glutamate transporter inhibitors enhance the antitumor efficacy of chemotherapeutic agents[J]. Biochim Biophys Acta, 2003, 1653(2): 47~59.
[9]	Zhang G, Miura Y, Yagasaki K.Effects of dietary powdered green tea and theanine on tumor growth and endogenous hyperlipidemia in hepatoma-bearing rats[J]. Biosci Biotechnol Biochem, 2002, 66(4): 711~716.
[10]	吕毅, 郭雯飞, 倪捷儿, 等. 茶氨酸的生理作用及合成[J]. 茶叶科学, 2003, 23(1): 1~5.
[11]	李平, 宛晓春, 张正竹. 生物合成茶氨酸的研究进展及构建基因工程菌合成茶氨酸的可行性探讨[J]. 食品与发酵工业, 2003: 71~75.
[12]	Suzuki H, Izuka S, Miyakawa N, et al. Enzymatic production of theanine, an “umami” component of tea, from glutamine and ethylamine with bacterialγ-glutamyltraspeptidase[J]. Enzyme and Microbial Technology, 2002, 31(6): 884~889.
[13]	J.萨姆布鲁克, D W, 拉塞尔. 分子克隆实验指南[M]. 黄培堂, 王嘉玺, 朱厚基, 等译. 北京: 科学出版社(第3版), 2002.
[14]	林智, 杨勇, 谭俊峰, 等. 茶氨酸提取纯化工艺研究[J]. 天然产物研究与开发, 2004, 16(5): 442~447.
[15]	Suzuki H, Kumagai H, Tochikura T.γ-Glutamyltranspeptidase from Escherichia coli K-12: purification and properties[J]. Journal of Bacteriology, 1986, 168(3): 1325~1331.

Research on Conditions of Theanine Biosynthesis by Genetically Engineered Escherichia coli

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 15

Related Articles 15

Metrics

Comments

Recommended 10

[1]	WANG Yuyuan, LIU Renjian, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Expression Analysis and Functional Identification of CsTT2 R2R3-MYB Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2022, 42(4): 463-476.
[2]	WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang. Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2022, 42(3): 331-346.
[3]	YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress [J]. Journal of Tea Science, 2022, 42(2): 187-199.
[4]	YAN Yuting, LI Yujie, WANG Qian, TANG Meijun, GUO Huawei, LI Hongliang, SUN Liang. The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren [J]. Journal of Tea Science, 2022, 42(2): 200-210.
[5]	ZHOU Hanchen, LEI Pandeng. The Functional Identification of Two Alternative Splicing Transcripts of CsNES [J]. Journal of Tea Science, 2021, 41(6): 753-760.
[6]	TANG Lei, XIAO Luodan, HUANG Yifan, XIAO Bin, GONG Chunmei. Cloning and Magnesium Transport Function Analysis of CsMGTs Genes in Tea Plants (Camellia sinensis) [J]. Journal of Tea Science, 2021, 41(6): 761-776.
[7]	HUANG Qiuping, XIE Chenyang, LI Xinyu, JIN Bin, ZENG Zhen, QIAN Bo, DAI Ling, SONG Jiale. L-Theanine Ameliorated Clinical Symptoms in Letrozole-Induced Polycystic Ovary Syndrome Rats [J]. Journal of Tea Science, 2021, 41(6): 831-842.
[8]	LIU Miaomiao, ZANG Liansheng, SUN Xiaoling, ZHOU Zhongshi, YE Meng. Cloning and Expression Analysis of CsWRKY17 Transcription Factor in Tea Plants [J]. Journal of Tea Science, 2021, 41(5): 631-642.
[9]	YAN Yuting, WU Fan, ZHANG Yali, FU Xiaobin, CUI Hongchun, HAN Baoyu, LI Hongliang. Study on the Differences in Ligand-binding Function and Mode of the Antennal High-abundance Odorant-binding Proteins EoblOBP9 and EoblOBP11 of the Tea Geometrid, Ectropis obliqua Prout [J]. Journal of Tea Science, 2021, 41(5): 643-653.
[10]	LIN Xinying, WANG Pengjie, CHEN Xuejin, GUO Yongchun, GU Mengya, ZHENG Yucheng, YE Naixing. Identification of LOX Gene Family in Camellia sinensis and Expression Analysis in the Process of White Tea Withering [J]. Journal of Tea Science, 2021, 41(4): 482-496.
[11]	WANG Yanding, WANG Huan, LI Nana, WANG Lu, HAO Xinyuan, WANG Yuchun, DING Changqing, YANG Yajun, WANG Xinchao, QIAN Wenjun. Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis [J]. Journal of Tea Science, 2021, 41(4): 497-510.
[12]	CHEN Meiyan, LIU Fen, LIN Yong, ZUO Gaolong, ZUO Yingpeng, LIU Zhonghua. Intervention Effects of L-Theanine on the Damage of Hippocampus and Gut in CUMS Depressed Rats [J]. Journal of Tea Science, 2021, 41(4): 511-524.
[13]	GU Mengya, WANG Pengjie, CHEN Xuejin, ZHENG Yucheng, GUO Yongchun, LIN Xinying, GAO Ting, HOU Binghao, YE Naixing. Identification of Alcohol Dehydrogenase Gene Family and Their Expression Analysis in the Withering Process of White Tea [J]. Journal of Tea Science, 2021, 41(3): 302-314.
[14]	CHEN Siwen, KANG Rui, GUO Zhiyuan, ZHOU Qiongqiong, FENG Jiancan. Cloning and Expression Analysis of CsCML16 in Tea Plants (Camellia sinensis) under Low Temperature Stress [J]. Journal of Tea Science, 2021, 41(3): 315-326.
[15]	MAO Peng, WANG Liyuan, BAI Peixian, WEI Kang, RUAN Li, ZHNAG Yazhen, CHENG Hao. Identification and Analysis of Analogue Theanine Synthase Gene Family in Tomato [J]. Journal of Tea Science, 2021, 41(2): 173-183.