A quantitative method for the determination of the total content of polyphenols in kudincha was studied by using FC method (Folin-Ciocalteu colorimetry ) with chlorogenic acid as standard. The results showed that the total polyphenol contents of kudincha could be well calculated according to their colorimetric absorption at 747 nm by applying Folin-Ciocalteu reagent 3.0 ml and saturated Na2CO3 4.5 ml at 30℃ for 30 min, and the linear range of standard curve was 20.0~100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0.887%~1.416% by RSD (relative standard deviation). There are not any differences for the results obtained by FC method and ferrous tartrate colourimetry. Therefore, FC method is convenient, fast, and accurate to determine the total polyphenols of kudincha.
LIU Li-xiang
,
Tanguy Laura
,
LIANG Xing-fei
,
SUN Yi
,
YE Hong
,
ZENG Xiao-xiong
. Determination of the Total Content of Polyphenols in Kudincha by Folin-Ciocalteu Colorimetry[J]. Journal of Tea Science, 2008
, 28(2)
: 101
-106
.
DOI: 10.13305/j.cnki.jts.2008.02.006
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