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Journal of Tea Science ›› 2008, Vol. 28 ›› Issue (2): 101-106.doi: 10.13305/j.cnki.jts.2008.02.006

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Determination of the Total Content of Polyphenols in Kudincha by Folin-Ciocalteu Colorimetry

LIU Li-xiang1, Tanguy Laura1,2, LIANG Xing-fei1, SUN Yi1, YE Hong1, ZENG Xiao-xiong1,*   

  1. 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Department of Food Engineering, Polytech’Lille, 59655 Villenevve d’Asqc, France
  • Received:2007-07-25 Revised:2007-12-07 Online:2008-04-15 Published:2019-09-16

Abstract: A quantitative method for the determination of the total content of polyphenols in kudincha was studied by using FC method (Folin-Ciocalteu colorimetry ) with chlorogenic acid as standard. The results showed that the total polyphenol contents of kudincha could be well calculated according to their colorimetric absorption at 747 nm by applying Folin-Ciocalteu reagent 3.0 ml and saturated Na2CO3 4.5 ml at 30℃ for 30 min, and the linear range of standard curve was 20.0~100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0.887%~1.416% by RSD (relative standard deviation). There are not any differences for the results obtained by FC method and ferrous tartrate colourimetry. Therefore, FC method is convenient, fast, and accurate to determine the total polyphenols of kudincha.

Key words: kudincha, polyphenol, Folin-Ciocalteu colorimetry

CLC Number: