To explore the protective effect of Fu brick tea on intestinal mucosal barrier and liver injury in rats with high-fat induced non-alcoholic fatty liver disease (NAFLD). SD male rats were randomly divided into normal, NAFLD, low-dose and high-dose Fu brick tea groups, 8 rats each group. The establishment of NAFLD rat model and the intervention effect of Fu brick tea were evaluated by analyzing the feed intake, weight change, blood lipid index, liver index and pathological section of rats. Intestinal integrity and intestinal permeability was assessed by pathological and PAS staining sections of jejunum and colon, villus height/crypt depth (V/C value) serum diamine oxidase (DAO) activity and lipopolysaccharide (LPS) activity. Intestinal-related inflammation was assessed by the number of jejunum intraepithelial lymphocytes (JIL) and the activity of serum tumor necrosis factor-alpha (TNF-α). The results show that Fu brick tea could reduce the feed intake andbody weight of NAFLD rats, decrease blood lipid levels, reduce the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, and inhibit lipid accumulation and inflammation in the liver. Fu brick tea also reduced the levels of serum inflammatory factors TNF-α and serum LPS and increased the activity of serum DAO. Moreover, Fu brick tea inhibited inflammatory cell infiltration in jejunum and colon villi of NAFLD rats, reduced the jejunum villus shedding, fracture, sparse and disorder, increased the jejunum villus V/C value, and decreased the number of jejunum intraepithelial lymphocytes, and increased the number of goblet cells in the jejunum and colon. These data indicate that Fu brick tea could effectively improve the intestinal mucosal barrier and liver injury induced by high-fat diet, which could be used to prevent and treat NAFLD.
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