Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)

CHENG Kaixin; YANG Kaixin; DENG Yayuan; LI Xin; LIU Enbei; WANG Yuchun; L&#x000dc; Wuyun

doi:10.13305/j.cnki.jts.2023.01.004

Journal of Tea Science >

2023 , Vol. 43 >Issue 1: 55 - 66

DOI: https://doi.org/10.13305/j.cnki.jts.2023.01.004

Research Paper

Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)

CHENG Kaixin ,
YANG Kaixin ,
DENG Yayuan ,
LI Xin ,
LIU Enbei ,
WANG Yuchun ,
LÜ Wuyun

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1. College of Tea Science and Tea Culture, Zhejiang A & F University, Hangzhou 311300, China;
2. Tea Research Institute of the Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China

Received date: 2022-10-27

Revised date: 2022-12-14

Online published: 2023-03-01

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Abstract

Colletotrichum camelliae is the dominant species causing foliar diseases of tea plants (Camellia sinensis). In this study, 65 isolates of C. camelliae were obtained from diseased leaves of tea plants in the 13 main tea-producing provinces of China. Phylogenetic analysis based on the multigene sequences was performed on all isolates, combined with their characteristics of colony and conidia, showed that all isolates clustered with C. camelliae. Pathogenicity analysis performed on detached leaves showed that all isolates of C. camelliae could infect the tea leaves, but the virulence of the isolates displayed a significant difference. The pathogenicity of 12 isolates, including YCW1180, YCW1331, YCW1382, YCW1387, YCW1419, YCW1443, YCW1451, YCW1453, YCW1454, YCW1461, YCW1613 and YCW2134, was significantly stronger than that of other isolates. By contrast, the pathogenicity of YCW1378 isolate was the weakest. The sensitivity of 65 isolates to 25% pyraclostrobin was determined by analyzing the inhibition rate of mycelial growth. The results show that the inhibition rate of pyraclostrobin on mycelial growth of YCW1436 isolate was only 36.00%, showing the worst antimicrobial effect. However, the inhibition rate of mycelial growth of most isolates was higher than 70.00%, which suggests that most isolates were highly sensitive to 25% pyraclostrobin. Microscopic observation shows that this agent mainly affected the conidial germination of C. camelliae. Overall, this study provided a solid foundation for the effective control of the diseases.

Key words： Colletotrichum camelliae; tea plants; strain identification; pathogenicity; fungicide sensitivity

Cite this article

CHENG Kaixin , YANG Kaixin , DENG Yayuan , LI Xin , LIU Enbei , WANG Yuchun , LÜ Wuyun . Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2023 , 43(1) : 55 -66 . DOI: 10.13305/j.cnki.jts.2023.01.004

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