To investigate the structure and activity of polysaccharides (MTP06), the polysaccharides were extracted and purified from Maojian tea. The refined Maojian tea polysaccharides (MP) were obtained after removing proteins. Different column chromatography methods were used for several times to purify the MP and an uniform polysaccharide named MTP06 was obtained. The effects of MTP06 on DPPH radical-scavenging activity, cell viability, engulfment capability and NO production of RAW264.7 macrophages were investigated . The results showed that the free radical scavenging rate of MTP06 to 0.1 mmol·L-1 DPPH solution was 30.85%. The highest viability, engulfment capability and NO production of RAW264.7 macrophages had been stimulated by MTP06 at 500 mg·L-1. The present results confirmed MTP06 can eliminate DPPH radicals and stimulate cell viabilities.
FAN Haitao
,
LIU Zhuang
,
ZHANG Zhaokang
,
CHENG Xiangyi
,
QIAO Shanyi
. Purification and Activity Determination of Polysaccharides MTP06 from Maojian Tea[J]. Journal of Tea Science, 2016
, 36(5)
: 531
-536
.
DOI: 10.13305/j.cnki.jts.2016.05.012
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