Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5): 531-536.doi: 10.13305/j.cnki.jts.2016.05.012

Previous Articles     Next Articles

Purification and Activity Determination of Polysaccharides MTP06 from Maojian Tea

FAN Haitao1,2, LIU Zhuang3, ZHANG Zhaokang4, CHENG Xiangyi4, QIAO Shanyi2,*   

  1. 1. College of Bioengineering, Beijing Polytechnic, Beijing 100176, China;
    2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences , Beijing 100850, China;
    3. Hospital of Beijing Union University, Beijing 100101, China;
    4. Department of Physics and Institute of Biophysics, Central China Normal University, Wuhan 100191, China
  • Received:2016-02-15 Online:2016-10-15 Published:2019-08-26

Abstract: To investigate the structure and activity of polysaccharides (MTP06), the polysaccharides were extracted and purified from Maojian tea. The refined Maojian tea polysaccharides (MP) were obtained after removing proteins. Different column chromatography methods were used for several times to purify the MP and an uniform polysaccharide named MTP06 was obtained. The effects of MTP06 on DPPH radical-scavenging activity, cell viability, engulfment capability and NO production of RAW264.7 macrophages were investigated . The results showed that the free radical scavenging rate of MTP06 to 0.1 mmol·L-1 DPPH solution was 30.85%. The highest viability, engulfment capability and NO production of RAW264.7 macrophages had been stimulated by MTP06 at 500 mg·L-1. The present results confirmed MTP06 can eliminate DPPH radicals and stimulate cell viabilities.

Key words: Maojian tea, polysaccharides, activity, macrophages

CLC Number: