In this study, ninhydrin/titanium dioxide nanomaterials were constructed by using Sol-Gel method. These nanomaterial were then used as direct-reading colorimetric materials and printed on the surface loci of thin layer plate where theanine would migrate according to its retardation factor (Rf). In addition, ethanol: acetic acid developing system was chosen as developing reagent, the extraction solution of tea after being decolorized by ethyl acetate was analyzed by thin layer chromatography (TLC) via direct-reading colorimetric reaction. The results showed that the current direct-reading strip could effectively separate several amino acids in tea extraction under optimized conditions. The new constructed colorimetric nanomaterials had achieved a sensitive direct-reading coloration results for the target amino acid theanine, and the color spots on the strip were uniform and clear after self-coloration. Moreover, its color depth had a clear semi-quantitative relationship with its concentration. The current colorimetric strip has a serial of advantages including safety, easy-operation, convenient and low cost, and has a broad application prospect in fields such as quality identification of agricultural products and on-site rapid detection of amino acids.
ZHU Chengbo
,
WU Xiaomei
,
FENG Lixia
,
HUANG Xiangpeng
,
PANG Linjiang
,
YANG Huqing
,
FANG Zhengwei
,
WANG Ying
,
LAI Shuyu
,
ZHANG Yiming
. Rapid Detection of Theanine in Green Tea Based on Direct-reading Colorimetric Strips[J]. Journal of Tea Science, 2018
, 38(3)
: 244
-252
.
DOI: 10.13305/j.cnki.jts.2018.03.004
[1] Haskell CF, Kennedy DO, Milne AL, et al.The effects of L-theanine, caffeine and their combination on cognition and mood[J]. Biological Psychology, 2008, 77(2): 113-122.
[2] Wang HF, Tsai YS, Lin ML, et al, Comparison of bioactive components in GABA tea and green tea produced in Taiwan[J]. Food Chemistry 2006, 96(4): 648-653.
[3] Scharbert S, Hofmann T.Molecular definition of black tea taste by means of quantitative studies, taste reconstitution, and omission experiments[J]. Journal of Agricultural and Food Chemistry, 2005, 53: 5377-5384.
[4] 毛清黎. 茶叶氨基酸的研究进展[J]. 氨基酸和生物资源, 1989(4): 16-21.
[5] 程启坤, 阮宇成, 王月根, 等. 绿茶滋味化学鉴定法[J]. 茶叶科学, 1985, 5(1): 7-17.
[6] 刘爽, 杨停, 谭俊峰, 等. 绿茶滋味定量描述分析及其化学成分的相关性研究[J]. 中国农学通报, 2014, 30(24): 40-46.
[7] Ruan JY, Gerendas J, Hardter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[8] Deng WW, Ogita S, Ashihara H, Biosynthesis of theanine (gamma-ethylamino-L-glutamic acid) in seedlings of Camellia sinensis[J]. Phytochemistry Letters, 2008, 1(2): 115-119.
[9] Oh K, Kato T, Xu HL.Transport of nitrogen assimilation in xylem vessels of green tea plants fed with NH4-N and NO3-N[J]. Pedosphere, 2008, 18(2): 222-226.
[10] Ruan J, Haerdter R, Gerendas J.Impact of nitrogen supply on carbon/nitrogen allocation: a case study on amino acids and catechins in green tea [Camellia sinensis (L.) O. Kuntze] plants[J]. Plant Biology, 2010(12): 724-734.
[11] Sasaoka K, Kito M, Onishi Y.Some properties of theanine synthesizing enzyme in tea seedlings[J]. Agricultural and Biological Chemistry, 1965, 29(11): 984-988.
[12] 黄建琴. 氨基酸在茶叶制造中的转化机理及对茶叶品质的影响[J]. 氨基酸和生物资源, 1992(1): 26-29.
[13] Wood PL, Khan MA, Moskal JR.Neurochemical analysis of amino acids, polyamines and carboxylic acids: GC-MS quantitation of tBDMS derivatives using ammonia positive chemical ionization[J]. Journal of Chromatography B, 2006, 831: 313-319.
[14] Thippeswamy R, Gouda KGM, Rao DH, et al.Determination of theanine in commercial tea by liquid chromatography with fluorescence and diode array ultraviolet detection[J]. Journal of Agricultural and Food Chemistry, 2006, 54(19): 7014-7019.
[15] 杨四润, 张冬莲, 方乔慧, 等. γ-氨基丁酸茶薄层检测方法的建立[J]. 食品安全质量检测学报, 2012, 3(1): 33-39.
[16] 王督, 张文, 李培武, 等. 胶体金免疫层析法快速定量分析粮油农产品中黄曲霉毒素B1[J]. 中国油料作物学报, 2014, 36(4): 529-532.
[17] 张锂. 薄层层析酶化学法测定马拉硫磷残留[J]. 兰州工业学院学报, 2017, 24(6): 82-85.
[18] 刘顺字, 郭淑贞, 林双娣, 等. 酶抑制法在农药残留快速检测过程中的应用分析[J]. 湖南农业科学, 2017(7): 85-87, 91.
[19] 杨丽, 尤丽, 叶金秀, 等. 纸层析分离鉴定氨基酸实验的改进[J]. 云南民族大学学报(自然科学版), 2011, 20(3): 229-231
[20] 王海龙, 崔智慧, 岳秀兰. 氨基酸薄层层析茚三酮显色改良[J]. 包头医学院学报, 2003, 19(4): 312-360.
[21] 马雪泷, 邹鹏飞, 王荡强, 等. 纸层析-分光光度法检测茶氨酸[J]. 植物生理学报, 2012, 48(4): 413-417.
[22] 张宜明, 叶海鹏, 李顺, 等. 茚三酮/纳米二氧化钛复合物及其制备方法和用途: CN201510817477.3[P]. 2016-01-27[2017-12-05]. 茚三酮/纳米二氧化钛复合物及其制备方法和用途: CN201510817477.3[P]. 2016-01-27[2017-12-05]. http://www.wanfangdata.com.cn/details/detail.do?_type=patent&id=CN201510817477.3.
[23] 阮宇成, 王月根. 茶氨酸的简化定量法[J]. 茶叶通讯. 1981(3): 41-45.
[24] 张丹丹, 叶小辉, 赵峰, 等. 基于游离氨基酸组分的白茶滋味品质研究[J]. 福建农业学报, 2016, 31(5): 515-520.
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