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Basic Information about the Journal
Journal title: Journal of Tea science
Inscription of journal title: ZHU De
Responsible Institution: China Association for Science and Technology
Sponsored by: China Tea Science Society
Tea Research Institute, Chinese Academy of Agricultural Science
Editing and Publishing: Editorial Office, Journal of Tea science
Start time: 1964
No. of issues: Bi-monthly
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    Research Advances of Fluoride Accumulation Mechanisms in Tea Plants (Camellia sinensis)
    XING Anqi, WU Zichen, XU Xiaohan, SUN Yi, WANG Genmei, WANG Yuhua
    Journal of Tea Science    2022, 42 (3): 301-315.   DOI: 10.13305/j.cnki.jts.20220416.003
    Abstract548)      PDF(pc) (876KB)(302)       Save
    Camellia sinensis (L.) O. Kuntze is a hyper fluoride (F) accumulation plant, whose F content in tea leaves is much higher than other plants, without any toxic symptoms. However, F is not an essential element for tea plant growth, and under high F stress, F affects the normal growth of plants by destroying the cell structure and inhibiting enzyme activities. In order to provide a theoretical basis for the future study of F accumulation in tea plants, the research progresses in the absorption, enrichment and accumulation/detoxification mechanisms of F in tea plants were reviewed.
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    Research Progress of Tea Quality Evaluation Technology
    LIU Qi, OUYANG Jian, LIU Changwei, CHEN Hongyu, LI Juan, XIONG Ligui, LIU Zhonghua, HUANG Jian'an
    Journal of Tea Science    2022, 42 (3): 316-330.   DOI: 10.13305/j.cnki.jts.20220416.001
    Abstract545)      PDF(pc) (812KB)(491)       Save
    The quality of tea is the embodiment of the shape and inner quality of tea, and evaluating the quality of tea quickly and accurately is essential for tea trade and processing. Sensory evaluation, composition analysis and detection, and emerging technologies are the main tea quality evaluation techniques at present. This article summarized the research progress of three major evaluation technologies in recent years, and focused on the development trend of emerging technologies. Sensory evaluation is greatly influenced by subjective factors, but combined with quantitative description and analysis can reduce the influence of subjectivity. Component analysis detection has high threshold, difficult operation, time-consuming and labor-consuming, and the results obtained are relatively accurate. Emerging technologies are simple, fast, and non-destructive, but at present they cannot achieve satisfactory accuracy. In the tea product diversification today, only multi-dimensional comprehensive utilization of multiple methods can quickly and efficiently detect the quality of tea, and provide assistance for the healthy and efficient development of the tea industry.
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    Identification and Transcriptional Regulation of CLH Gene Family and Expression Analysis in Albino Tea Plants (Camellia sinensis)
    WANG Tao, WANG Yiqing, QI Siyu, ZHOU Zhe, CHEN Zhidan, SUN Weijiang
    Journal of Tea Science    2022, 42 (3): 331-346.   DOI: 10.13305/j.cnki.jts.2022.03.003
    Abstract391)      PDF(pc) (1540KB)(121)       Save
    Chlorophyllase (CLH) is the key enzyme in the degradation of chlorophyll, stripping its phytol to form dephytolithochlorophyll a. The full-length cDNA sequences of three CsCLHs genes were obtained from the second leaves of albino tea cultivar ‘Baijiguan', and bioinformatics analysis was performed. The results show that the three CsCLH genes could be divided into two subfamilies. The full length of CsCLHs was 894-975 bp, encoding 297-324 amino acids. The protein molecular weights were 31.99-34.91 kDa. The isoelectric points were 4.89-7.61, and the instability coefficients were 38.94-48.24. CsCLH1.1 and CsCLH1.2 were unstable proteins, while CsCLH2 was a stable protein. The subcellular localization prediction results of Cell Ploc show that three CsCLH proteins were located in chloroplast, while the results of Wolf Psort show that CsCLH1.1 and CsCLH1.2 were located in cytoplasm and CsCLH2 was located in chloroplast. The qRT-PCR results on the ‘Baijiguan' leaves indicated that expressions of CsCLHs were inhibited by shading treatment and light induced CsCLHs' expressions. Expression pattern analysis of CsCLHs shows that CsCLH1s were highly expressed in the albino cultivars. In addition, it was identified that CsCDF5 could bind to the CsCLH1.1 and CsCLH2 promoters according to the yeast one hybrid system. In conclusion, CsCLHs in albino tea leaves might be involved in chlorophyll degradation and play an important role in the process of albino leaf, which provided a reference for further investigation in the function of the CLH gene family and the albinism of leaves in tea plants.
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    Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants
    LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng
    Journal of Tea Science    2022, 42 (3): 347-357.   DOI: 10.13305/j.cnki.jts.2022.03.001
    Abstract261)      PDF(pc) (1629KB)(88)       Save
    Tea plant leaf trichomes contain various secondary metabolites and play an important role in the tea appearance quality as well as the response of tea plants to biotic and abiotic stresses. In this study, the function of leaf trichome-related genes CsbHLH024 and CsbHLH133 were analyzed using Bimolecular Fluorescent Complimentary (BiFC), GUS staining and overexpression experiments. The results show that CsbHLH024/CsbHLH133 and CsTTG1 could interact in plants, and their promoters could drive downstream gene expression in leaf tissues. They were further transformed into wild Arabidopsis thaliana (Col) and corresponding homozygous mutants, respectively to get overexpression lines. Both genes could affect the leaf trichome formation in Arabidopsis thaliana, restore the phenotype of the mutants, and induce the expression levels of trichome-related genes. This study provided a theoretical basis for further research on the molecular regulation mechanism of trichome formation in tea leaves.
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    The Control Efficiency of Afidopyropen to Tea Green Leafhoppers and Evaluation of Residue in Tea
    GUO Mingming, LI Zhaoqun, LIU Yan, RAO Fuqiang, YU Jiawei, WU Luchao, ZHOU Li, CHEN Zongmao
    Journal of Tea Science    2022, 42 (3): 358-366.   DOI: 10.13305/j.cnki.jts.20220506.002
    Abstract345)      PDF(pc) (505KB)(103)       Save
    Afidopyropen is a novel biogenic insecticide derived from the natural fermentation product. This study aimed to evaluate the feasibility of afidopyropen for tea green leafhoppers (Empoasca onukii Matsuda) control and tea safety after the application of afidopyropen in tea garden. The dosage field trials and demonstration trials were conducted to evaluate the control efficiency of afidopyropen to tea green leafhoppers and the terminal residues in tea. The results of the dosage field trials in 2 locations show that 50 g·L-1 afidopyropen dispersible concentrate was more effective against the green leafhoppers than chlorfenapyr which was commonly used pesticide for the green leafhoppers control. At the dosages of 15.00 g·hm-2 and 22.50 g·hm-2, control efficiencies were 88.6%-93.4% on the 1st day after treatment and 75.5%-85.5% on the 14th day, demonstrating a quick control effect and good persistence. At the dosage of 18.75 g·hm-2 in 7 locations, the control efficiencies were 88.9%-100.0% on the 3rd day after treatment, and 60.2%-100.0% on the 14th day, which were better than the local commonly used pesticides. The terminal residues of afidopyropen in green tea ranged from 0.17-0.64 mg·kg-1 on the 7th day after the application, the leaching rate of afidopyropen from dry tea to tea brew ranged from 17.1%-19.1% during the brewing process, and the risk quotient values were far less than 1, indicating a very low health risk caused by the afidopyropen intake through drinking tea. In conclusion, afidopyropen is suitable for resistance management and comprehensive management of tea green leafhoppers with the advantages of low dosage, high-efficiency and low-healthy risk for tea consumer.
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    The Antifungal Effect and Mechanism of Berberine on Different Colletotrichum Species Causing Tea Brown Blight Disease
    ZHANG Zhipeng, CHENG Qinghua, XIE Jingkang, WAN Yuhe, TONG Huarong, CHEN Yingjuan
    Journal of Tea Science    2022, 42 (3): 367-375.   DOI: 10.13305/j.cnki.jts.2022.03.006
    Abstract234)      PDF(pc) (2110KB)(85)       Save
    To explore the application prospects of berberine on the prevention and control of tea diseases, the antifungal activity of berberine against five different Colletotrichum species (C. fioriniae, C. karstii, C. chongqinggense, C. camelliae and C. gloeosporioides) were studied and the differences in antifungal activities were compared. The results show that berberine has the best inhibitory effect on both C. camelliae and C. chongqinggense, reaching 100% at the concentration of 12 mg·mL-1, followed by C. gloeosporioides, C. karstii and C. fioriniae. The EC50 (Concentration for 50% of maximal effect) of the five Colletotrichum species were 2.828, 3.288, 4.164, 4.778 mg·mL-1 and 5.104 mg·mL-1, respectively. The conidial and hyphal morphology of five Colletotrichum species under the treatment of berberine were examined, showing that berberine had a significant impact on the mycelial and conidial growth of five Colletotrichum species. With the increase of berberine concentration, part of hyphae expanded irregularly and the conidia grew abnormally. Results of the biological activity of Colletotrichum species show that the cell structure was damaged, the permeability of cell membrane was increased and the cell oxidative stress response was enhanced. Results of this study firstly confirm the antifungal activity and application prospects of berberine against Colletotrichum species, which provided a new approach and theoretical basis for the prevention and control of tea disease caused by Colletotrichum.
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    Research and Application of Intelligent Identification of Empoasca onukii Based on Machine Vision
    BIAN Lei, HE Xudong, JI Huihua, CAI Xiaoming, LUO Zongxiu, CHEN Huacai, CHEN Zongmao
    Journal of Tea Science    2022, 42 (3): 376-386.   DOI: 10.13305/j.cnki.jts.20220506.001
    Abstract215)      PDF(pc) (1047KB)(99)       Save
    Deep learning has been widely used in intelligent identification in the progress of real-time monitoring of crop pests. Based on the identification model of tea leafhopper, Empoasca onukii, the application of deep learning in field leafhopper recognition was introduced to improve the precision of field population investigation of E. onukii. In this paper, a method of identification and count of E. onukii in tea garden was designed. Firstly, yellow sticky card was used to attract tea leafhoppers, and images of cards were collected by camera and uploaded to the web server. Then, target detection algorithm deployed by the server was used to identify and count the leafhoppers in the images. Through algorithm screening, YOLOv3 was determined as the recognition algorithm, and the improved soft-NMS was used to replace the original NMS. K-means clustering method was used to calculate the size of the new prior frame, so as to improve the speed and precision of YOLOv3. The results show that the average precision of the optimized algorithm could reach more than 95.35% comparing with the real number of leafhoppers on the sticky card. Therefore, the combination of the sticky card trapping, target recognition algorithm and internet of things technology could realize the real-time monitoring of population for E. onukii, which could provide a reference for other insects with color preference and integrated pest management in tea gardens.
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    Research on the Classification Method of Tea Buds Combining Improved Capsule Network and Knowledge Distillation
    CHEN Xingran, HUANG Haisong, HAN Zhenggong, FAN Qingsong, ZHU Yunwei, HU Pengfei
    Journal of Tea Science    2022, 42 (3): 387-396.   DOI: 10.13305/j.cnki.jts.2022.03.002
    Abstract202)      PDF(pc) (750KB)(41)       Save
    The accurate classification of different grades of tea buds is very important for the development of the famous tea industry. The use of traditional sensory evaluation methods for sorting makes the results subjective. In this research, a data set was established after tea leaf images were collected, and a new network model, GA-CapsNet, was proposed by combining the ghost attention bottleneck and capsule network. The model was trained by the method of growing knowledge distillation based on the linear decay scaling coefficient, while migrating the parameter matrix of teacher model, the student model was adaptively reduced with iteration. The experimental result shows that, compared with other similar algorithms, the proposed method had excellent classification performance on small-scale data sets. The accuracy, recall and F1-score were 94.97%, 95.51% and 95.24%, respectively. Here, a GA-CapsNet model based on machine vision and deep learning technology was established, which provided a new idea for solving the tea leaf classification problem.
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    Characterization of the Key Aroma in Corn-scented Congou Black Tea Manufactured from Camellia nanchuanica by Sensory Omics Techniques
    OUYANG Ke, ZHANG Cheng, LIAO Xueli, KUN Jirui, TONG Huarong
    Journal of Tea Science    2022, 42 (3): 397-408.   DOI: 10.13305/j.cnki.jts.20220506.003
    Abstract260)      PDF(pc) (1290KB)(117)       Save
    Camellia nanchuanica is endemic to Nanchuan district, Chongqing and has a high development value. In this study, the characteristic aroma of corn-scented Congou black tea from Camellia nanchuanica was analyzed by headspace-solid phase microextraction, gas chromatography-mass spectrometry (HS-SPME-GC/MS), gas chromatography-olfactometry (GC-O), odor activity value (OAV) and aroma character impact value (ACI). The results show that 22 key aroma compounds with OAVs≥1 were identified in the corn-scented black tea. Dimethyl sulfide had the highest OAV (1 187.32), and its ACI was 51.56%. A total of 26 odor-active compounds were perceived by GC-O. Dimethyl sulfide had a higher olfactory intensity, which had a “corn-like” odor. The results obtained by the OAV approaches and by GC-O method for key aroma identification were in good agreement. The importance of dimethyl sulfide in corn-scented black tea was verified by aroma recombination and omission test. Focusing on the analysis of the active aromas of corn-scented black tea would provide a theoretical basis for the quality control of this prized tea.
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    Development of Amplitude Adjustable Imitation Manual Rocking Green Device for Oolong Tea and Research of Process Parameters
    BIAN Xianbing, WU Min, JIA Jiangming, DONG Chunwang, ZHANG Chen'an, ZHAO Runmao, CHEN Jianneng, WANG Jinshuang
    Journal of Tea Science    2022, 42 (3): 409-422.   DOI: 10.13305/j.cnki.jts.2022.03.005
    Abstract213)      PDF(pc) (1967KB)(79)       Save
    To better promote the large-scale standardized processing of oolong tea, improve the rocking green quality and machinery efficiency, and extend the application scope of equipment, an amplitude adjustable imitation manual rocking green device and a single input multi output power transmission mechanism were designed. The kinematics analysis model of the amplitude adjustable rocking green mechanism was established, and the spatial motion trajectory of the spherical screen was simulated by MATLAB. On this basis, a rocking green prototype was developed to carry out the multi index orthogonal test of 2 factors and 4 levels of the rocking green. Then, the multi index was transformed into a single index by comprehensive scoring method, and the best process parameters were btained by the orthogonal test analysis. Under the optimal process parameters, the moderate rate of oolong tea rocking green was 84.10%, the water loss rate was 33.63%, and the comprehensive score was 97.75% of the traditional manual rocking green. The experimental results show that the developed amplitude adjustable imitation manual rocking green mechanism is effective. On the basis of matching the relevant process parameters, it can be applied to the large-scale mechanical rocking green of fresh leaves in different tenderness and seasons. Meanwhile, it also provided a new reference method for the research of relevant process parameters in the field of tea processing.
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    Econometric Analyses of EGCG Research Literature
    ZHANG Yini, JI Zheng
    Journal of Tea Science    2022, 42 (3): 423-434.   DOI: 10.13305/j.cnki.jts.20220416.002
    Abstract202)      PDF(pc) (1222KB)(111)       Save
    The epigallocatechin gallate (EGCG) has shown the highest biological activity in tea components in most studies, which has shown beneficial effects on anti-cancer, bacterial and viral infections. A review of the advances and hot spots of EGCG studies were summarized through citation analysis. In order to ensure that the review is objective and scientific, literature analysis was performed from the core collection of the data base of ISI Web of Science of the period of 2000-2021 with the key words “EGCG” and “Epigallocatechin gallate”. A total of 6 799 relevant papers were obtained. Then, the CiteSpace visualization software was used for citation analysis and the research hotspots and frontier trends were studied through network analysis including cooperation, co-occurrence and co-citation. Statistical analysis of volume of the publications shows that China, United States and Japan ranked the top three in this field. Analysis of betweenness central indicators shows that United States, Germany and China were the top three in terms of importance. Further citation analysis shows that “nanoparticle”, and “stability” were the hotspots in the recent years. With the deepening of the research on EGCG, it was speculated that the future research would focus on improving the bioavailability of EGCG and exploring the optimal dose level and administration frequency.
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    Study on the Preference of Large Yellow Tea Based on Consumers with Different Ages
    AN Qi, QIU Tong, ZHANG Ying, XIAO Mingji, CHEN Aini, SHENG Caiyan, DAI Qianying
    Journal of Tea Science    2022, 42 (3): 435-446.   DOI: 10.13305/j.cnki.jts.2022.03.004
    Abstract232)      PDF(pc) (3196KB)(105)       Save
    In this paper, 9-point hedonic scale and 5-point just about right were used to test the preference of large yellow tea and evaluate the acceptance of sensory attributes among consumers with different ages. The results show that young and middle-aged consumers prefer low-roasted and high-tenderness large yellow tea with sweet and mellow flavors while older consumers prefer high-roasted and low-tenderness large yellow tea with the burnt, bitter and paste flavors. Combined the JAR data with the preference data through penalty analysis, the most influential attributes, the direction of attribute optimization and the order of improvement priority were qualitatively obtained from the three age groups, which was of certain reference to design and process large yellow tea products.
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    Camellia Ptilophylla and Specific Chemical Components, Theirs Health Beneficial Effects
    WU Wenliang, TONG Tong, HU Yao, ZHOU Hao, YIN Xia, ZHANG Shuguang
    Journal of Tea Science    2021, 41 (5): 593-607.   DOI: 10.13305/j.cnki.jts.20210917.002
    Abstract635)      PDF(pc) (1145KB)(333)       Save
    Cocoa tea (Camellia ptilophylla Chang) is a specific tea resource in China, and theobromine (TB) and gallocatechin gallate (GCG), as the dominant chemical components of cocoa tea, have various health effects. This paper summarized intervention effects and mechanisms of cocoa tea, TB and GCG on cardiovascular diseases, cancers, obesity, diabetes, neurodegenerative diseases, dental diseases, respiratory diseases and kidney diseases, etc. This review would provide a theoretical reference for the development of functional cocoa tea products.
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    Analysis on the Achievements of Tea Science and Technology Awards at Provincial and Ministerial Level and above in China from 2008 to 2019
    XIONG Xingping, ZHANG Xinzhong, LI Hongbin, SUN Liang, YAO Mingzhe
    Journal of Tea Science    2021, 41 (5): 608-618.   DOI: 10.13305/j.cnki.jts.20210917.001
    Abstract481)      PDF(pc) (1013KB)(255)       Save
    Based on the national, provincial and ministerial science and technology awards in the field of tea science in China from 2008 to 2019, statistical analyses on the award, grade, year of award, the first completed person, the first completed unit, the main research fields, and the cooperation between industry, university and research unit were performed. The current status, characteristics and support trends of tea science and technology awards in China were clarified, aiming to provide ideas for Chinese tea science and technology personnel to cultivate achievement, determine scientific research topics and apply for awards.
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    Analysis of Genetic Diversity and Population Structure of 32 Tea Landraces in China
    LI Changle, GE Yue, YAN Meilin, LI Hui, LIN Qingqing, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang
    Journal of Tea Science    2021, 41 (5): 619-630.   DOI: 10.13305/j.cnki.jts.20210804.002
    Abstract328)      PDF(pc) (1077KB)(203)       Save
    Tea plant populations are natural populations of tea plants. They are cultivated in specific production areas and contain individuals with different economic and biological traits. There are great differences among individuals and there are many types. They are important materials for studying the evolutionary relationship and breeding of tea plants. In this study, 30 pairs of SSR primers distributed on 15 linkage groups were selected to analyze the genetic diversity and population structure of 32 tea plant populations from 12 provinces, in order to provide a reference for the selection of tea breeding parents and the inference of evolution route. In this study, a total of 149 alleles were obtained with an average of 5.96 for each SSR marker. The average polymorphism information content of primers was 0.660. Shannon's diversity index of 32 tea populations ranged from 0.691 to 1.089, with an average of 0.954. The observed heterozygosity ranged from 0.253 to 0.633, with an average of 0.510. The expected heterozygosity ranged from 0.430 to 0.653, with an average of 0.590. The average genetic differentiation coefficient (Fst) of tea plant population was 0.205, indicating a high level of genetic differentiation. The results of clustering based on Nei's genetic distance and population structure analysis were consistent. The germplasm to be tested was divided into 4 major types, with obvious regional distribution.
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    Cloning and Expression Analysis of CsWRKY17 Transcription Factor in Tea Plants
    LIU Miaomiao, ZANG Liansheng, SUN Xiaoling, ZHOU Zhongshi, YE Meng
    Journal of Tea Science    2021, 41 (5): 631-642.   DOI: 10.13305/j.cnki.jts.2021.05.001
    Abstract391)      PDF(pc) (1820KB)(127)       Save
    WRKY transcription factors, a super family of plant transcription factors, play an essential role in the regulation of plant defense responses to herbivores. While the roles of herbivore-related WRKY transcription factors are well established in grass plants, their roles in woody plants are still largely unknown. Here, we cloned a WRKY transcription factor, named CsWRKY17. CsWRKY17 has a full length of 1 141 bp, contains a 987 bp open reading frame, and encodes 328 amino acids. Based on the conserved domain analysis, CsWRKY17 belongs to the WRKY Ⅱ subfamily, containing one conserved WRKY domain and a typical C2H2-type zinc finger motif. Homology alignment and phylogenetic tree analysis show that CsWRKY17 has the closest relationship with AtWKRY11 and AtWRKY17 in Arabidopsis thaliana. Moreover, CsWRKY17 exhibited a tissue specific expression, and was also induced by mechanical wounding, tea geometrid (Ectropis oblique) attack, simulated herbivory, and exogenous phytohormone treatments like JA. Transient expression experiments indicate that it might play a role in the nucleus. Taken together, we proposed that CsWRKY17 is a potential regulator of herbivore-induced defense responses against herbivores in tea plants through JA, ABA, GA and BR signaling. Our study paved the way for molecular analysis of herbivore-related WRKY genes in tea plants, and provided a good genetic resource and theoretical basis for future studies of pest-resistant genes and breeding of tea plants.
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    Study on the Differences in Ligand-binding Function and Mode of the Antennal High-abundance Odorant-binding Proteins EoblOBP9 and EoblOBP11 of the Tea Geometrid, Ectropis obliqua Prout
    YAN Yuting, WU Fan, ZHANG Yali, FU Xiaobin, CUI Hongchun, HAN Baoyu, LI Hongliang
    Journal of Tea Science    2021, 41 (5): 643-653.   DOI: 10.13305/j.cnki.jts.2021.05.002
    Abstract241)      PDF(pc) (1230KB)(64)       Save
    As an important lepidopteran pest in tea gardens, the male and female adults of Ectropis obliqua Prout can perceive and distinguish the chemical information of the external environment through the olfactory system, which helps its behaviors such as mating and selection of oviposition locations. In this process, Odorant-binding proteins (OBPs) highly expressed in the antennae of adults may play an important role. In view of this, this study focused on the two OBPs high expressed in the antennae of the adult tea geometrid, namely EoblOBP9 and EoblOBP 11. First, it was found that both EoblOBP9 and EoblOBP11 contain six conserved cysteines, the typical characteristics of the OBPs family, while the isoelectric points are predicted to be acidic and basic, respectively, indicating their difference in the primary structural properties. Both recombinant proteins were obtained using prokaryotic expression technology, and the corresponding polyclonal antibodies were prepared by immunizing mice. It was verified that they were indeed expressed specifically in the antennae of male adults by Western blot. Their binding abilities with 20 candidate ligands were tested by fluorescence competitive binding experiments, and the results show that both of them were compatible with the two sex pheromone components (Z3,Z9-6,7-epo-18: Hy and Z3,Z6,Z9-18: Hy) and a plant volatile component (trans-2-hexenal) has a strong affinity. Meanwhile, two OBPs also showed different ligand binding spectra. For example, EoblOBP9 had a strong affinity with 1-penten-3-ol, benzyl alcohol, acetophenone and benzaldehyde, indicating that the binding profiles were wider; while EoblOBP11 had a strong affinity with α-terpineol with a more specific binding mode. Molecular docking analysis also supports this conclusion. In addition, it was predicted that the active sites of both proteins are located at the C-terminus, which also shows the commonality of ligand binding. In conclusion, this study shows that although both OBPs were specifically expressed in the adult tea geometrid with high abundance, they are both conservative and different in terms of structural properties and biochemical binding profiles. The general characters and significant differences are coexist, showing the high abundance and diversities of the OBPs. This can also explain an adaptation mechanism of the tea geometrid olfactory system in the face of the complex and changeable external environment.
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    Decomposition Characteristics of Lignin and Cellulose in Different Litters of Ecological Tea Gardens in Mountainous Areas of Guizhou
    LIU Shaqian, YANG Rui, HOU Chunlan, MA Juebing, GUO Jiarui
    Journal of Tea Science    2021, 41 (5): 654-668.   DOI: 10.13305/j.cnki.jts.2021.05.003
    Abstract332)      PDF(pc) (588KB)(103)       Save
    The litter decomposition bag method was used to analyze the decomposition of five single litter types and nine mixed litter types in Guizhou Jiu'an Ecological Tea Garden with Cinnamomum glanduliferum, Betula luminifera, Cunninghamia lanceolata and Pinus massoniana leaf litters and Camellia sinensis trims as the research objects. The decomposition rate and release characteristics and differences of lignin and cellulose were studied. The results show that in a single type of litter, the litter decomposition rate of broad-leaved species was higher than that of coniferous species. The lignin content of litter of broad-leaved species showed an increasing trend. While that of coniferous species showed a downward trend as a whole. The content of cellulose in litter also showed a decreasing trend. According to the analysis of the release law of lignin and cellulose, both single litter release law and mixed litter cellulose release law were direct release. There were three release laws for single litter lignin, and 5 laws for mixed litter lignin. According to the analysis of the decomposition characteristics of 9 kinds of mixed litter, 44.4% of mixed litter decomposition showed additive effect, 11.2% mixed decomposition showed antagonistic effect, and 44.4% mixed decomposition showed synergistic effect. While 22.2% of mixed litter lignin release showed promoting release, and 55.6% mixed litter lignin release promoted the enrichment. A total of 11.1% of the mixed litter cellulose appeared to promote the enrichment, and 22.2% of the mixed litter cellulose was to promote the release. This study provided a theoretical basis for further maintaining the fertility in tea gardens.
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    Preventive Effect of Green Brick Tea on Non-alcoholic Fatty Liver Disease via Gut Microbiota Changes
    ZHOU Tingting, CHEN Guiting, CAO Nan, HE Jiangang, HE Gongwei, XIAO Changyi, LI Shigang
    Journal of Tea Science    2021, 41 (5): 669-680.   DOI: 10.13305/j.cnki.jts.20210914.001
    Abstract270)      PDF(pc) (4419KB)(94)       Save
    The preventive effect of green brick tea (GBT) on the mouse non-alcoholic fatty liver disease (NAFLD) model was studied by affecting changes in gut microbiota. C57BL/6 mice were randomly divided into 5 groups, including normal control group (NC), model control group (MC), positive drug control group (PC), low-dose group (LD) and high-dose group (HD) of GBT. A NAFLD model was established by feeding mice with high-fat diet, and supplemented with low and high doses of GBT water extract and positive drug (Xuezhikang) respectively. The body weight, food utilization efficiency, liver weight, liver index, TC, LDL-C/HDL-C and ALT contents of mice were determined. Liver tissue pathological sections were observed by HE staining and Oil Red O staining. ELISA method was used to detect changes of IL-1β and IL-18 in liver tissue. The changes of gut microbiota were analyzed by high-throughput sequencing in 16 S rDNA V3-V4 region, and Spearman's correlation analysis method was used to analyze the correlation between gut microbiota and NAFLD phenotype. Compared with the model control group, the body weight, food utilization efficiency, liver weight, liver index, serum TC, LDL-C/HDL-C, ALT, liver tissue TC, IL-1β, and IL-18 contents of mice in the GBT group were significantly reduced, and the degree of liver disease was improved. Gut microbiota analysis and correlation analysis show that the species abundance of Bacteroides decreased, and it was positively correlated with the NAFLD phenotype. The species abundance of Lactobacillus, Alloprevotella, and Saccharibacteria_genera_incertae_sedis increased, and they were negatively correlated with the NAFLD phenotype. Bacteroides and Lactobacillus had the strongest correlation with NAFLD phenotype. Green brick tea has a certain preventive effect on NAFLD, and its effect may be related to the changes in gut microbiota.
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    Effects of Fu Brick Tea on Inflammation and Intestinal Microflora Diversity in Mice with DSS-induced Ulcerative Colitis
    HUANG Xiangxiang, TAN Ting, YU Lijun, WANG Kunbo, HUANG Jian'an, XU Shiyu, LIU Zhonghua
    Journal of Tea Science    2021, 41 (5): 681-694.   DOI: 10.13305/j.cnki.jts.2021.05.004
    Abstract245)      PDF(pc) (3509KB)(100)       Save
    In this study, we investigated the effects of Fu brick tea on the anti-inflammation and intestinal microflora of ulcerative colitis (UC) mice induced by Dextran sulfate sodium (DSS). Sixty C57BL/6 female mice were randomly divided into the normal control group, 10 mg·kg-1 EGCG control group, high dose brick tea (300 mg·kg-1, HBT) control group, DSS model group, DSS+10 mg·kg-1 EGCG group, DSS+low dose brick tea (100 mg·kg-1, LBT) group, DSS+middle dose brick tea (150 mg·kg-1, MBT) group and DSS+HBT group, with the control groups n=5 and the treatment groups n=9. After 1 week of DSS modeling, the mice were gavaged for 4 weeks. The mice were executed after 5 weeks of the treatments. The histopathological changes of mice colon were observed, and the disease activity index (DAI) of mice colitis was assessed. The levels of IL-6, IL-8, IL-1β, TNF-α and IFN-γ inflammatory factors in mice serum and the expression levels of JAK2 and STAT3 genes in mice colon tissues were measured. The intestinal microflora of mice was analyzed by PCR-DGGE and clone sequencing techniques. The results show that compared with the DSS model group, the quality of survival and colonic tissue morphology of mice were significantly improved after feeding EGCG and different concentrations of brick tea, and the levels of inflammatory factors IL-6, IL-8, IL-1β, TNF-α and IFN-γ in mice serum were significantly reduced (P<0.05). Moreover, the expressions of JAK2 and STAT3 genes were significantly reduced (P<0.05) JAK2/STAT3 inflammatory signaling pathway was inhibited; and intestinal microflora diversity and richness were increased significantly. The dominant bacterial flora in different treatment groups were changed as well. In conclusion, Fu brick tea can ameliorate DSS-induced UC injury by inhibiting JAK2/STAT3 inflammatory signaling pathway and modulating intestinal microflora diversity.
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