Abstract: A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1βµg/L to 10βµg/L. The detection limit of crude extract was 2βmg/kg and the purified was 0.06βmg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea.

Key words: endosulfan, ELISA, tea, GC/MS