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Journal of Tea Science ›› 2006, Vol. 26 ›› Issue (2): 141-146.doi: 10.13305/j.cnki.jts.2006.02.013

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Determination of Endosulfan and Endosulfan Sulfate in Tea by ELISA

LU Xiao1, 2, REN Feng-shan2, WANG Wen-bo2, DU Hong-xia2, LI Hui-dong2, DING Rui-yan2, ZHU Duan-wei1, LEE Nanju A3, SHEILD Ross4, KENNEDY Ivan R5   

  1. 1. Faculty of Natural Resources & Environment, Huazhong Agricultural University, Wuhan 430070, China;
    2. Central Laboratory, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China;
    3. Department of Food Science & Technology, University of NSW, Sydney, NSW, 2050, Australia;
    4. Agrisearch Analytical, Sydney, NSW, 2039, Australia;
    5. Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW, 2006, Australia
  • Received:2005-12-12 Revised:2006-01-12 Online:2006-06-25 Published:2019-09-10

Abstract: A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1βµg/L to 10βµg/L. The detection limit of crude extract was 2βmg/kg and the purified was 0.06βmg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea.

Key words: endosulfan, ELISA, tea, GC/MS

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