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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5): 347-354.doi: 10.13305/j.cnki.jts.2009.5.004

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Molecular Cloning and Real-Time PCR Analysis of Flavone SynthaseⅡ Gene Full-length cDNA from the Tea Plant

QIAO Xiao-yan1,2, MA Chun-lei1, CHEN Liang1,*   

  1. 1. Research Center for Tea Germplasm and Improvement, Tea Research Institute of the Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China;
    2. Tea Research Institute of the Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
  • Received:2009-02-12 Revised:2009-04-09 Online:2009-10-15 Published:2019-09-09

Abstract: Using RT-PCR and RACE techniques, flavone synthase Ⅱ (FSⅡ) gene, the key enzyme in synthesize flavone, was cloned in the tea plant. The FS II belongs to cytochrome P450 superfamily. The GenBank Accession No. is FJ169499 in the NCBI. The FSⅡ gene cDNA had 1 824 bp in full-length with 1 605 bp in coding region, encoding 534 amino acids. The putative molecular weight was 60.4 kD. The expression levels of flavone synthaseⅡ gene in spring ‘two and a bud’, mature leaves, petals, stamen and pistils, and seeds of the tea plant were assessed using SYBR Green I Real-time PCR. The expression in mature leaves was significantly higher than that in the other four tissues.

Key words: tea plant, flavone synthaseⅡ, Real-Time PCR, cytochrome P450 superfamily

CLC Number: