Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis)

CHEN Yao; ZHANG Weifu; REN Hengze; XIONG Fei; ZHANG Haojie; YAO Lina; LIU ying; WANG Lu; WANG Xinchao; YANG Yajun; HAO Xinyuan

doi:10.13305/j.cnki.jts.2021.01.004

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (1) : 28-39. DOI: 10.13305/j.cnki.jts.2021.01.004

Research Paper

Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis)

CHEN Yao, ZHANG Weifu, REN Hengze, XIONG Fei, ZHANG Haojie, YAO Lina, LIU ying, WANG Lu, WANG Xinchao, YANG Yajun^*, HAO Xinyuan^*

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Abstract

DNA demethylase (dMTase) is a bifunctional enzyme with activities of glycosylase and apyrimidic lysase during active demethylation. Based on the available genome data of tea plant, bioinformatics analysis was used to comprehensively identify DNA demethylases (CsdMTase) in tea plant. The genome-wide identification results show that the tea cultivar Shuchazao contains 4 CsdMTases. These CsdMTases could be divided into two branches (DME and ROS) with differences in gene length and intron number by phylogenetic and gene structure analysis. Further analysis shows that the physical and chemical properties of different CsdMTase proteins are similar, which contain the typical conserved domains of ENCO3c and RRM_DME and are located in the nucleus. The promoter regions of CsdMTases contain a large number of cis-acting components related to light response, plant hormone response, stress response and growth and development. The CsdMTase genes are expressed in all detected tissues with certain tissue specificity. The expressions of CsdMTases were significantly up-regulated under biotic stresses, including Colletotrichum camelliae, Pseudopestalotiopsis camelliae-sinensis and Ectropis oblique treatments. During winter dormancy, different expression patterns of CsdMTases were detected in mature leaves and overwintering buds of different cultivars. The expressions of CsDMEa and CsDMEb at different stages of flower bud development and seed germination were significantly induced. The results show that active DNA demethylation process mediated by CsdMTases plays a crucial role in regulating the stress responses, growth and development of tea plant, providing a theoretical basis for discovering the epigenetic regulation mechanism in tea plant.

Key words

DNA demethylase / expression analysis / growth and development / stress response / tea plant

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CHEN Yao, ZHANG Weifu, REN Hengze, XIONG Fei, ZHANG Haojie, YAO Lina, LIU ying, WANG Lu, WANG Xinchao, YANG Yajun, HAO Xinyuan. Genome-wide Investigation and Expression Analysis of DNA Demethylase Genes in Tea Plant (Camellia Sinensis)[J]. Journal of Tea Science. 2021, 41(1): 28-39 https://doi.org/10.13305/j.cnki.jts.2021.01.004

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