Analysis of Glutathione S-transferase Genes in Tea Plant (Camellia sinensis) Based on Transcriptome Analysis

ZHANG Yazhen; WEI Kang; WANG Liyuan; CHENG Hao

doi:10.13305/j.cnki.jts.2016.05.010

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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5) : 513-522. DOI: 10.13305/j.cnki.jts.2016.05.010

Analysis of Glutathione S-transferase Genes in Tea Plant (Camellia sinensis) Based on Transcriptome Analysis

ZHANG Yazhen, WEI Kang^*, WANG Liyuan, CHENG Hao

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Abstract

Glutathione S-transferases (GSTs) belong to a superfamily of multifunctional enzymes and are ubiquitous in plants. Forty nine CsGSTs genes were identified by transcriptome analysis of Zhonghuang 2 and Longjing 43 under control and shading treatment. Nineteen CsGSTs with relatively high expression levels in buds were used for sequence and phylogenetic tree analysis. Real-time fluorescence quantitative PCR (qRT-PCR) was performed to analyze the expression patterns of 8 candidate genes in different leaf positions of Longjing 43. The results showed that CsGSTs were expressed in all tested leaves, but exhibited different expression patterns. CsGST20 showed an increasing expression from a bud with a leaf to the sixth leaf, suggesting a potential role in stress resistance. On the other hand, the expression level of CsGST24 dropped significantly, suggesting that CsGST24 might participate in anthocyanin accumulation in tea plants.

Key words

expression analysis / GST / tea plant (Camellia sinensis) / transcriptome analysis

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ZHANG Yazhen, WEI Kang, WANG Liyuan, CHENG Hao. Analysis of Glutathione S-transferase Genes in Tea Plant (Camellia sinensis) Based on Transcriptome Analysis[J]. Journal of Tea Science. 2016, 36(5): 513-522 https://doi.org/10.13305/j.cnki.jts.2016.05.010

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