基于转录组测序对茶树GST基因表达的研究

doi:10.13305/j.cnki.jts.2016.05.010

Abstract

Abstract: Glutathione S-transferases (GSTs) belong to a superfamily of multifunctional enzymes and are ubiquitous in plants. Forty nine CsGSTs genes were identified by transcriptome analysis of Zhonghuang 2 and Longjing 43 under control and shading treatment. Nineteen CsGSTs with relatively high expression levels in buds were used for sequence and phylogenetic tree analysis. Real-time fluorescence quantitative PCR (qRT-PCR) was performed to analyze the expression patterns of 8 candidate genes in different leaf positions of Longjing 43. The results showed that CsGSTs were expressed in all tested leaves, but exhibited different expression patterns. CsGST20 showed an increasing expression from a bud with a leaf to the sixth leaf, suggesting a potential role in stress resistance. On the other hand, the expression level of CsGST24 dropped significantly, suggesting that CsGST24 might participate in anthocyanin accumulation in tea plants.

Key words: tea plant (Camellia sinensis), transcriptome analysis, GST, expression analysis

CLC Number:

S571.1
Q51

ZHANG Yazhen, WEI Kang, WANG Liyuan, CHENG Hao. Analysis of Glutathione S-transferase Genes in Tea Plant (Camellia sinensis) Based on Transcriptome Analysis[J]. Journal of Tea Science, 2016, 36(5): 513-522.

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Analysis of Glutathione S-transferase Genes in Tea Plant (Camellia sinensis) Based on Transcriptome Analysis

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