湖南益阳黑茶中桔青霉线粒体全基因组序列测定及分析

doi:10.13305/j.cnki.jts.2020.06.010

Abstract

Abstract: In this study, the mitochondrial genome sequence of a strain of Penicillium citrinum isolated from dark tea was determined and analyzed, and its phylogenetic relationship with the closely related microorganisms was explored. The result shows that the mitochondrial genome of Penicillium citrinum is a circular DNA molecule with a length of 27β537βbp, which encodes 42 genes. The genome bases are composed of A (36.17%), T (37.06%), C (11.82%) and G (14.95%). All the 15 protein-coding genes use typical ATG as the start codon, TAA or TAG as the stop codon. Its gene sequences are similar to those of the reported Penicillium species and are conserved in evolution. The highly occurred amino acids in the protein-coding genes are Leu, Ile, Ser and Phe. The top 4 codons with the high RSCU frequency are UUA, AUA, UUU and GGU, respectively. There are 30 G-U mismatches in 24 tRNA genes, all of which could form typical cloverleaf structure. Phylogenetic analysis shows that the most closely related taxonomic status of Penicillium citrinum is Penicillium ShG4C, followed by Penicillium polonicum and Penicillium nordicum.

Key words: dark tea, Penicillium citrinum, mitochondrial genome, phylogeny

CLC Number:

HU Zhiyuan, LIU Suchun, XU Zhenggang, LIU Shiquan, WEN Xin. Sequencing and Analysis of the Complete Mitochondrial Genome of Penicillium Citrinum in Hunan Yiyang Dark Tea[J]. Journal of Tea Science, 2020, 40(6): 830-844.

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Sequencing and Analysis of the Complete Mitochondrial Genome of Penicillium Citrinum in Hunan Yiyang Dark Tea

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