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茶叶科学 ›› 2012, Vol. 32 ›› Issue (5): 441-447.doi: 10.13305/j.cnki.jts.2012.05.001

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EGCG甲基化衍生物酶促合成的反应条件研究

吕海鹏, 费冬梅, 张悦, 林智*, 彭群华, 郭丽, 谭俊峰   

  1. 国家茶产业工程技术研究中心,中国农业科学院茶叶研究所, 浙江 杭州 310008
  • 收稿日期:2012-03-07 修回日期:2012-03-31 出版日期:2012-10-15 发布日期:2019-09-05
  • 通讯作者: linz@mail.tricaas.com
  • 作者简介:吕海鹏(1980— ),男,山东沾化人,助理研究员,主要从事茶叶加工化学研究。
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项(2012zl053)、国家自然科学基金项目(30972404)、现代农业产业技术体系建设专项资金资助(CARS-23)

Study on the Reaction Condition of EGCG Methylated Derivatives Produced from EGCG Catalyzed by EGCG-O-Methyltransferase

LV Hai-peng, FEI Dong-mei, ZHANG Yue, LIN Zhi*, PENG Qun-hua, GUO Li, TAN Jun-feng   

  1. National Engineering Research Center for Tea Industry, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2012-03-07 Revised:2012-03-31 Online:2012-10-15 Published:2019-09-05

摘要: 以EGCG-O-甲基转移酶催化EGCG生成EGCG3′′Me、EGCG4′′Me和EGCG3′Me等3种主要的EGCG甲基化衍生物的生成量为主要指标,考察了EGCG甲基化衍生物酶促合成的反应条件。结果表明,EGCG甲基化衍生物的酶促合成最佳反应条件为:温度35℃,pH7.5,DTT和Mg2+的浓度为2mmol/L;在该反应条件下,反应体系中EGCG3′′Me、EGCG4′′Me以及EGCG3′Me的生成量分别可达到386.39μg/mL、23.40μg/mL和107.01μg/mL。

关键词: EGCG甲基化衍生物, 酶促合成, EGCG-O-甲基转移酶

Abstract: Taken the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me as the main index, the investigation on the reaction condition of EGCG methylated derivatives produced from EGCG catalyzed by EGCG-O-Methyltransferase were conducted. Results showed that the optimum reaction condition was as follows: the concentration of DTT and Mg2+ was 2mmol/L, the initial pH was 7.5 and the reaction temperature was 35℃. Under the above conditions, the yields of EGCG3"Me, EGCG4′′Me and EGCG3′Me could reach 386.39μg/mL, 23.40μg/mL and 107.01μg/mL, respectively.

Key words: EGCG methylated derivatives, enzymatic synthesis, EGCG-O-Methyltransferase

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