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茶叶科学 ›› 2013, Vol. 33 ›› Issue (1): 27-33.doi: 10.13305/j.cnki.jts.2013.01.001

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复合酶解辅助乙醇提取茶多酚工艺研究

谢蓝华, 肖南, 张嘉怡, 江东文, 杜冰*, 杨公明   

  1. 华南农业大学食品学院,广东 广州 510642
  • 收稿日期:2012-04-05 修回日期:2012-05-22 出版日期:2013-02-28 发布日期:2019-09-04
  • 通讯作者: *gzdubing@163.com
  • 作者简介:谢蓝华(1987— ),男,湖南攸县人,硕士研究生,主要从事食品生物技术与微生物研究。
  • 基金资助:
    广东省产学研结合项目(2011B090400071)、广东省教育部产学研结合项目“低值单枞茶深加工及综合利用”(2009B090300138)

Study on Composite-enzymolysis Assisted Ethanol Extraction of Tea Polyphenols

XIE Lan-hua, XIAO Nan, ZHANG Jia-yi, JIANG Dong-wen, DU Bing*, YANG Gong-ming   

  1. College of Food, South China Agricultural University, Guangzhou 510642, China
  • Received:2012-04-05 Revised:2012-05-22 Online:2013-02-28 Published:2019-09-04

摘要: 以单枞茶为实验材料,研究复合酶解辅助乙醇提取茶多酚的工艺条件,考察酶解温度、酶添加量、酶解pH、酶解时间、乙醇质量分数、料液比、提取时间、提取温度8个单因素对茶多酚提取率的影响。在单因素实验的基础上,通过正交实验优化提取工艺参数。结果表明:酶解温度60℃、酶添加量3.5%、酶解pH4.8的条件下酶解预处理3βh后,在乙醇质量分数为60%、提取温度为70℃、料液比为1︰50的条件下提取1.5βh,茶多酚的提取率达25.82%,与传统水提法相比,茶多酚提取率提高了69.87%。

关键词: 茶多酚, 复合酶解, 醇提

Abstract: Composite-enzymolysis assisted ethanol extraction of tea polyphenols from Danzong-tea was optimized in this investigation. The effects of factors including enzymolysis temperature, enzyme content, enzymolysis pH, enzymolysis time, ethanol concentration, solid-to-liquid ratio,extraction temperature, extraction time on the extraction rates of tea polyphenols were explored by single-factor and orthogonal test. The results showed that the optimal extraction conditions were after enzyming pretreatment in the conditions of enzymatic content 3.5%, enzymolysis temperature 60℃, enzymolysis pH 4.8, enzymolysis time 3 h, then 60% ethanol to extract for 1.5 h with extraction temperature70℃ and solid-to-liquid ratio 1︰50, tea polyphenols extraction in Danzong-tea was up to 25.82% and ncreased 69.87% compared with the traditional water extraction.

Key words: tea polyphenols, composite- enzymolysis, ethanol extraction

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