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茶叶科学 ›› 2013, Vol. 33 ›› Issue (6): 562-569.doi: 10.13305/j.cnki.jts.2013.06.007

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英德市茶园土壤昆虫病原真菌群落结构与多样性研究

王品1, 黄翠1, 黎健龙2,3, 李增智1, 王滨1,*   

  1. 1. 安徽农业大学,安徽 合肥230036;
    2. 广东省农业科学院茶叶研究所,广东 英德 513042;
    3. 华南农业大学,广东 广州 510640
  • 收稿日期:2013-02-18 修回日期:2013-03-26 出版日期:2013-12-30 发布日期:2019-09-04
  • 通讯作者: *bwang@ahau.edu.cn
  • 作者简介:王品(1986— ),女,安徽六安人,硕士,主要从事生物防治工作。
  • 基金资助:
    国家863项目(2011AA10A203、2011AA10A204)、环保公益性行业科研专项(201009023)、科技基础性工作专项2008FY210500、广东省科技计划项目 (2010B031700029)

The Community Structure and Diversity of Entomopathogenic Fungi in the Tea Garden Soil of Guangdong Province

WANG Pin1, HUANG Cui1, LI Jian-long2,3, LI Zeng-zhi1, WANG Bin1,*   

  1. 1. Anhui Agricultural University, Hefei 230036, China;
    2. Tea Research Institute, Guangdong Academy of Agricultural Sciences, Yingde 513042, China;
    3. South China Agricultural University, Guangzhou 510640, China
  • Received:2013-02-18 Revised:2013-03-26 Online:2013-12-30 Published:2019-09-04

摘要: 土壤是昆虫病原真菌的重要贮存库,本文利用选择性培养基标准平板技术,对广东省英德市4个茶园土壤中昆虫病原真菌的物种多样性与群落结构进行了研究。结果显示:茶园土壤中共分离出了4个属8个种的昆虫病原真菌;其中以淡紫拟青霉最多,占90.20%;金龟子绿僵菌其次,占5.60%。昆虫病原真菌多样性随季节变化而变化,春夏两季多样性指数较高,分别为0.7148和0.6918;秋冬两季多样性指数较低,分别是0.4940和0.4302。茶园植被类型越复杂,土壤中昆虫病原真菌的物种多样性也越高,分离总数也越多。单一茶树品种但有乔木层遮蔽的茶园,其多样性指数为1.0651,远远高于没有乔木层遮蔽茶园的0.3393。增加茶树的种植品种,可以增加茶园土壤中昆虫病原真菌的多样性与丰富度,多样性指数也可以提高。调整茶园植被类型对于茶园土壤中昆虫病原真菌群落结构的丰富与多样性的提升有着重要的生态作用。

关键词: 茶园, 土壤, 昆虫病原真菌, 群落结构, 生物多样性

Abstract: Soil is an important reservoir of entomopathogenic fungi in the fields. The community structure and species diversity of the entomopathogenic fungi in the soil of four tea gardens in Yingde, Guangdong province was investigated. Four genus and 8 species of entomogenous fungi was identified, and their population dynamics were monitored by the selective medium technique. The results showed Paecilomyces lilacinus dominated the community by a population percentage of 90.20% to the total, and followed by Metarhizium anisopliae of 5.60%. Community diversity of the entomogenous fungi changed with seasons. The brillouin diversity index reached peak at a value of 0.7148 and 0.6918 in spring and in summer, and decreased to 0.4940 and 0.4302 in autumn and in winter, respectively. The more complex of plant structure in the tea gardens showed, the higher biological diversity of entomogeneous fungi in the soil of the tea gardens was found. The monocultured tea garden shaded by tree layer had a diversity index of 1.0651, which far higher than that of 0.3393 of the garden without tree layer. More cultivars of tea bush in a garden increase the diversity and richness of entomopathogenic fungi in the soil. The diversity index was also increased. Plant structure played an important ecological role in increasing diversity of an entomopathogenic fungi community in a tea garden soil.

Key words: tea garden, soil, entomopathogenic fungi, community structure, biological diversity

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