氮素转化菌对茶树根际土壤微生物群落和养分含量的影响

doi:10.13305/j.cnki.jts.2015.05.001

茶叶科学 ›› 2015, Vol. 35 ›› Issue (5): 405-414.doi: 10.13305/j.cnki.jts.2015.05.001

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氮素转化菌对茶树根际土壤微生物群落和养分含量的影响

韩晓阳^1,2, 张丽霞^1,2,*, 黄晓琴^1,2, 董玉惠^1,2, 李智^1,2, 尚涛³

1. 山东农业大学园艺科学与工程学院,山东泰安 271018;
2. 作物生物学国家重点实验室,山东泰安 271018;
3. 泰安市农业局,山东泰安 271000

收稿日期:2015-04-20 修回日期:2015-07-06 出版日期:2015-10-15 发布日期:2019-08-26
通讯作者: *zlx_sdau@163.com
作者简介:韩晓阳,男,博士,主要从事茶树生理生态研究。
基金资助:
“十二五”国家科技支撑计划（2011BAD11B01、2011BAD11B02）、泰安生态茶园关键技术集成研究及产业化示范（201340629）、山东农业大学青年科技创新基金（02023826）资助

Effect of Nitrogen Transformation Bacteria on Microbial Community and Nutrient Contents in Rhizosphere Soil of Tea Plant

HAN Xiaoyang^1,2, ZHANG Lixia^1,2,*, HUANG Xiaoqin^1,2, DONG Yuhui^1,2, LI Zhi^1,2, SHANG Tao³

1. College of Horticulture Science and Engineering, Shandong Agriculture University, Taian 271018, China;
2. State Key Laboratory of Crop Biology, Taian 271018, China;
3. Agriculture Bureau of Taian City, Taian 271000, China

Received:2015-04-20 Revised:2015-07-06 Online:2015-10-15 Published:2019-08-26

摘要/Abstract

摘要： 为了研究氮素转化菌对茶树根际土壤微生态环境的影响,本试验以山东泰安2年生茶树根际土壤为研究对象,设置枯草芽孢杆菌（T1）和褐球固氮菌（T2）两个接种处理,以不接种为对照（CK）。采用培养计数法与末端限制性片段长度多态性技术（T-RFLP）,测定不同时期根际土壤中细菌、真菌、放线菌、氨化细菌、固氮菌、硝化细菌的数量及接种后微生物多样性的变化。同时测定接种后土壤中养分含量的变化。结果表明,接种60βd后T1和T2处理细菌数量显著高于CK,但对真菌及放线菌的影响不显著。整个试验过程氨化细菌和固氮菌数量高于CK,氨化细菌以T1处理最多,固氮菌以T2处理最多,硝化细菌数量始终是CK显著高于T1和T2处理。基于T-RFLP图谱分析,与对照组相比,菌剂组均增加了土壤微生物多样性和丰富度,各物种在数量上的均匀程度变大。在土壤养分方面,T1、T2菌剂处理组铵态氮浓度显著高于对照组,硝态氮含量低于对照。在速效P方面,T1、T2含量比CK分别显著提高了8%、25%。在速效K方面,T1、T2含量显著高于CK,提高了15%和11%。枯草芽孢杆菌和褐球固氮菌菌剂改善了茶树根际土壤微生态环境,提高了土壤养分,表现出正面效应,是一种生态安全性较高的生物菌剂。

关键词: 固氮菌, 氨化细菌, T-RFLP, 微生物多样性, 土壤养分

Abstract: The effects of nitrogen transformation bacteria on micro-ecological environment of the rhizosphere soil of 2 years old tea plant were studied in Taian City of Shandong Province. The Bacillus subtilis (T1) and Azotobacter chroococcum (T2) were set up as inoculation treatments, and the noninoculation as the control in this research. The number of bacteria, fungi, actinomycetes, ammonifying bacteria, azotobacter, nitrifying bacteria and the modification of microbial diversity in rhizosphere soil in different periods were measured, using the methods of culture counting and T-RFLP. Meanwhile, the nutrient contents in the soil were also determined. The results showed that the bacterial number of T1 and T2 were significantly higher than CK, but there were no significant impact on fungi and actinomycetes. In the whole test process, the number of ammonifying bacteria and azotobacter were higher than that of CK, among which ammonifying bacteria of T1 was the most, azotobacter of T2 was the most; the nitrifying bacteria numbers of CK was significantly higher than T1 and T2. Based on T-RFLP mapping analysis and compared with the control group, microbial diversity, the richness and evenness were rise in the bacterium treatments. The ammonium nitrogen concentration of T1 and T2 were significantly higher than that of CK, but the nitrate concentration were lower than that of CK. The available P concentration of T1 and T2 significantly increased by 8% and 25% for CK. Meanwhile, the available K concentration of T1 and T2 significantly increased by 15% and 11% for CK. The inoculation of Bacillus subtilis and Azotobacter chroococcum showed the positive effects, which could improve micro-ecological environment and soil nutrients of tea rhizospheric soil.

Key words: nitrogen fixing bacteria, ammonifying bacteria, T-RFLP, microbial diversity, soil nutrient

中图分类号:

韩晓阳, 张丽霞, 黄晓琴, 董玉惠, 李智, 尚涛. 氮素转化菌对茶树根际土壤微生物群落和养分含量的影响[J]. 茶叶科学, 2015, 35(5): 405-414. doi: 10.13305/j.cnki.jts.2015.05.001.

HAN Xiaoyang, ZHANG Lixia, HUANG Xiaoqin, DONG Yuhui, LI Zhi, SHANG Tao. Effect of Nitrogen Transformation Bacteria on Microbial Community and Nutrient Contents in Rhizosphere Soil of Tea Plant[J]. Journal of Tea Science, 2015, 35(5): 405-414. doi: 10.13305/j.cnki.jts.2015.05.001.

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氮素转化菌对茶树根际土壤微生物群落和养分含量的影响

Effect of Nitrogen Transformation Bacteria on Microbial Community and Nutrient Contents in Rhizosphere Soil of Tea Plant

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