茶园芽孢杆菌QM7促生特性及耐酸铝机制初步研究

doi:10.13305/j.cnki.jts.2016.06.003

摘要/Abstract

摘要： 目前含芽孢杆菌等微生物制剂的有机肥料在酸性茶园土壤中的生物活性不高,难以起到有效的促生作用,达到部分替代和减少化肥使用量的目的。本实验分离得到1株耐酸铝芽孢杆菌,命名为QM7。试验发现菌株促生能力受到铝离子浓度的影响,在无铝条件下菌株生长素（IAA）的分泌量为85.6βmg·L^-1,当铝离子浓度为1 mmol·L^-1时IAA分泌量为36.8 mg·L^-1;盆栽结果表明,在6周内菌株可以增加茶苗根系表面积56.8%,根尖数27.3%;蛋白电泳分析发现高浓度的铝胁迫会导致菌株胞内64种与转录、翻译和代谢相关蛋白的表达发生差异,使得菌株生长受到抑制。

关键词: 茶园土壤, 耐酸铝芽孢杆菌, 生长素（IAA）, 蛋白电泳

Abstract: At present, the biological activities of Bacillus.spp and other microorganisms in the acid tea garden soil are not high, thereby difficult to effectively promote plant growth and replace or reduce the usage of chemical fertilizer. In this study, one of Aluminum (Al) tolerance strains, Bacillus subtilis QM7 was isolated from tea garden soil. The auxin (IAA) production of QM7 was influenced by Al concentration (from 85.6 mg·L^-1 to 36.8 mg·L^-1under 0 to 1 mmol·L^-1 external Al respectively). Results of pot experiment showed that strain QM7 could promote plant growth by increasing root surface area by 56.8% and numbers of root tips by 27.3% in 6 weeks. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins involved in Al toxicity-induced changes. Results showed that more than 64 proteins differentially expressed in response to Al stress, including proteins involved in DNA transcription, protein translation and cell envelope, which reduced the growth of Bacillus subtilis.

Key words: tea planting soil, aluminum tolerance Bacillus subtilis, auxin (IAA), two-dimensional gel electrophoresis

中图分类号:

罗毅, 苏有健, 张永利, 夏先江, 宋莉, 王烨军, 廖万有. 茶园芽孢杆菌QM7促生特性及耐酸铝机制初步研究[J]. 茶叶科学, 2016, 36(6): 567-574. doi: 10.13305/j.cnki.jts.2016.06.003.

LUO Yi, SU Youjian, ZHANG Yongli, XIA Xianjiang, SONG Li, WANG Yejun, LIAO Wangyou. Research on The Promoting Effects on Tea Growth and Aluminum Tolerant Mechanism of Bacillus subtilis Strain QM7[J]. Journal of Tea Science, 2016, 36(6): 567-574. doi: 10.13305/j.cnki.jts.2016.06.003.

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