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茶叶科学 ›› 2022, Vol. 42 ›› Issue (1): 29-40.

• 研究报告 • 上一篇    下一篇

基于UPLC技术解析金萱×紫娟F1分离群体代谢物的遗传变异

刘庆帅1,2, 璩馥榕1,2, 魏梦园1, 钟红1,2, 王熠1,2, 陈亮1, 金基强1,*   

  1. 1.中国农业科学院茶叶研究所/国家茶树改良中心/农业农村部茶树生物学与资源利用重点实验室,浙江 杭州 310008;
    2.中国农业科学院研究生院,北京 100081
  • 收稿日期:2021-10-09 修回日期:2021-11-12 出版日期:2022-02-15 发布日期:2022-02-18
  • 通讯作者: *xyjjq@tricaas.com
  • 作者简介:刘庆帅,男,硕士研究生,主要从事茶树资源育种与遗传改良研究。
  • 基金资助:
    浙江省自然科学基金(LZ22C160008)、国家自然科学基金(31670685)、中国农业科学院科技创新工程(CAASASTIP-2017-TRICAAS)、财政部和农业农村部:现代农业产业技术体系(CARS-19)、中央科研院所基本科研业务费(1610212018008)

The Genetic Variation of the Chemical Components of the ‘Jinxuan' × ‘Zijuan' F1 Segregating Population Based on UPLC

LIU Qingshuai1,2, QU Furong1,2, WEI Mengyuan1, ZHONG Hong1,2, WANG Yi1,2, CHEN Liang1, JIN Jiqiang1,*   

  1. 1. Tea Research Institute of the Chinese of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agriculture Science, Beijing 100081, China
  • Received:2021-10-09 Revised:2021-11-12 Online:2022-02-15 Published:2022-02-18

摘要: 为创制高甲基化儿茶素茶树新种质,以金萱和紫娟为亲本构建了一个F1代分离群体,并通过建立一种超高效液相色谱法,对群体内各单株的代谢物含量及其遗传变异情况进行分析。研究发现,群体中多数代谢物含量分布符合正态偏陡分布,变异系数在20%~30%,且均存在一定数量的超亲个体,并从中筛选出9份高甲基化儿茶素等富含功能成分的优异单株。同时,结果显示多数代谢物含量在秋季高于春季,且儿茶素总量随叶片紫化程度加深而减少。本研究建立的超高效液相色谱法,为今后鉴定和筛选优异茶树种质资源和育种材料提供了一种更高效的测定方法;对金萱×紫娟F1分离群体生化组分的遗传变异研究也为今后高功能成分育种和通过正向遗传学发掘调控相关性状的基因提供了重要基础。

关键词: 茶树, 超高效液相色谱法, F1分离群体, 代谢物, 遗传变异

Abstract: In order to innovate new germplasms with high methylated catechins, a F1 segregating population was constructed with ‘Jinxuan' and ‘Zijuan' as parents. At the same time, an ultra-high performance liquid chromatography method was established to analyze the metabolite content and genetic variation of individual plants in the population. Our study found that most metabolites conformed to the normal and slightly steep distribution. The coefficient of variations were between 20% and 30%. There was a clear heterosis and several individual plants rich in methylated catechins were selected from the population. It also found that the contents of most metabolites in autumn were higher than those in spring, and the amount of total catechins decreased with the purple degree of leaves. The UPLC technology established in this study would provide a more efficient determination method for the future identification and screening of elite tea germplasms and breeding materials. The genetic variation of the chemical components of the ‘Jinxuan' בZijuan' F1 segregating population identified in this study also provided an important foundation for the high functional component breeding and discovery of genes governing traits through forward genetics.

Key words: tea, ultra high performance liquid chromatography, F1 population, metabolites, genetic variation

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