基于UPLC技术解析金萱&#x000D7;紫娟F1分离群体代谢物的遗传变异

刘庆帅; 璩馥榕; 魏梦园; 钟红; 王熠; 陈亮; 金基强

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茶叶科学 ›› 2022, Vol. 42 ›› Issue (1) : 29-40.

研究报告

基于UPLC技术解析金萱×紫娟F₁分离群体代谢物的遗传变异

刘庆帅^1,2, 璩馥榕^1,2, 魏梦园¹, 钟红^1,2, 王熠^1,2, 陈亮¹, 金基强^1,*

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The Genetic Variation of the Chemical Components of the ‘Jinxuan' × ‘Zijuan' F₁ Segregating Population Based on UPLC

LIU Qingshuai^1,2, QU Furong^1,2, WEI Mengyuan¹, ZHONG Hong^1,2, WANG Yi^1,2, CHEN Liang¹, JIN Jiqiang^1,*

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文章历史 +

摘要

为创制高甲基化儿茶素茶树新种质,以金萱和紫娟为亲本构建了一个F₁代分离群体,并通过建立一种超高效液相色谱法,对群体内各单株的代谢物含量及其遗传变异情况进行分析。研究发现,群体中多数代谢物含量分布符合正态偏陡分布,变异系数在20%~30%,且均存在一定数量的超亲个体,并从中筛选出9份高甲基化儿茶素等富含功能成分的优异单株。同时,结果显示多数代谢物含量在秋季高于春季,且儿茶素总量随叶片紫化程度加深而减少。本研究建立的超高效液相色谱法,为今后鉴定和筛选优异茶树种质资源和育种材料提供了一种更高效的测定方法;对金萱×紫娟F₁分离群体生化组分的遗传变异研究也为今后高功能成分育种和通过正向遗传学发掘调控相关性状的基因提供了重要基础。

Abstract

In order to innovate new germplasms with high methylated catechins, a F₁ segregating population was constructed with ‘Jinxuan' and ‘Zijuan' as parents. At the same time, an ultra-high performance liquid chromatography method was established to analyze the metabolite content and genetic variation of individual plants in the population. Our study found that most metabolites conformed to the normal and slightly steep distribution. The coefficient of variations were between 20% and 30%. There was a clear heterosis and several individual plants rich in methylated catechins were selected from the population. It also found that the contents of most metabolites in autumn were higher than those in spring, and the amount of total catechins decreased with the purple degree of leaves. The UPLC technology established in this study would provide a more efficient determination method for the future identification and screening of elite tea germplasms and breeding materials. The genetic variation of the chemical components of the ‘Jinxuan' ×‘Zijuan' F₁ segregating population identified in this study also provided an important foundation for the high functional component breeding and discovery of genes governing traits through forward genetics.

导出引用

刘庆帅, 璩馥榕, 魏梦园, 钟红, 王熠, 陈亮, 金基强. 基于UPLC技术解析金萱×紫娟F₁分离群体代谢物的遗传变异[J]. 茶叶科学. 2022, 42(1): 29-40

LIU Qingshuai, QU Furong, WEI Mengyuan, ZHONG Hong, WANG Yi, CHEN Liang, JIN Jiqiang. The Genetic Variation of the Chemical Components of the ‘Jinxuan' × ‘Zijuan' F₁ Segregating Population Based on UPLC[J]. Journal of Tea Science. 2022, 42(1): 29-40

中图分类号： S571.1

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