茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究

代昕玥; 葛炳钢; 张旭雯; 刘文武; 段继春; 傅冬和

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茶叶科学 ›› 2022, Vol. 42 ›› Issue (1) : 63-75.

研究报告

茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究

代昕玥^1,2, 葛炳钢^1,2, 张旭雯^1,2, 刘文武^1,4, 段继春⁵, 傅冬和^1,2,3,*

作者信息 +

Effect of Fu Brick Tea on Improving Metabolic Disorders in Type 2 Diabetes Mice

DAI Xinyue^1,2, GE Binggang^1,2, ZHANG Xuwen^1,2, LIU Wenwu^1,4, DUAN Jichun⁵, FU Donghe^1,2,3,*

Author information +

文章历史 +

摘要

为探究茯砖茶能否通过调节肠道微生物群改善2型糖尿病（T2DM）的代谢紊乱,建立T2DM小鼠模型,给予400 mg·kg^-1茯砖茶水提取物灌胃干预,观测T2DM小鼠的饮食量、饮水量、空腹体重、空腹血糖、糖耐量等日常指标,血清中胰岛素（INS）、总胆汁酸（TBA）、总胆固醇（TC）、总甘油三酯（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平,以及胰腺、回肠的病理学变化,并对小鼠粪便进行了肠道菌群16 S rRNA测序。结果显示,茯砖茶显著改善了小鼠的糖脂代谢紊乱和炎症,明显修复了T2DM导致的胰腺和回肠损伤。茯砖茶能显著降低T2DM引起的疣微菌门相对丰度异常增加,恢复肠道微生物群多样性。茯砖茶能增加肠道厚壁菌门、Eubacterium_coprostanoligenes_group、肠杆菌属、Acetatifactor、双歧杆菌属、Prevotella_9、Faecalibacterium、A2、Coriobacteriaceae及Eggerthellaceae等有益菌,说明茯砖茶可通过增加肠道有益菌群,调节肠道菌群失调,进而改善T2DM的代谢紊乱。

Abstract

To investigate whether Fu brick tea (FBT) water extract can attenuate metabolic disorders in Type 2 Diabetes (T2DM) by regulating gut microbiota, a T2DM mouse model was established with streptozocin and 400 mg·kg^-1 FBT water extract administration. Diet, and water consumption, body weight, fasting glucose and glucose tolerance in mice were observed. Serum levels of insulin (INS), total bile acid (TBA), total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) were examined. Histopathological changes of ileum and pancreas were also observed. Furthermore, fecal samples were analyzed by 16 S rRNA gene sequencing. The results show that FBT reduced the serum lipid metabolism levels, blood glucose, and inflammatory cytokines. Simultaneously, FBT treatment significantly ameliorated pathological changes in the pancreas and ileum. Moreover, the diversity, structure and composition of T2DM-disrupted gut microbiota were restored by the supplementation of FBT. T2DM-induced increase in the relative abundance of Verrucomicrobia was remarkably restored by FBT. FBT increased the growth of many key beneficial bacteria, including Firmicutes, Bifidobacterium, Enterorhabdus, Prevotella_9, A2, Acetatifactor, Eubacterium_coprostanoligenes_group, Faecalibacterium, Coriobacteriaceae and Eggerthellaceae. Collectively, the study showed that FBT might alleviate dysbacteriosis and metabolic disorders in T2DM by increasing beneficial flora.

导出引用

代昕玥, 葛炳钢, 张旭雯, 刘文武, 段继春, 傅冬和. 茯砖茶改善2型糖尿病小鼠代谢紊乱的效果研究[J]. 茶叶科学. 2022, 42(1): 63-75

DAI Xinyue, GE Binggang, ZHANG Xuwen, LIU Wenwu, DUAN Jichun, FU Donghe. Effect of Fu Brick Tea on Improving Metabolic Disorders in Type 2 Diabetes Mice[J]. Journal of Tea Science. 2022, 42(1): 63-75

中图分类号： S571.1 R587.1

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