抹茶对记忆获得障碍模型小鼠的改善及机制分析

摘要/Abstract

摘要： 为探讨抹茶对东莨菪碱诱导的记忆获得障碍模型小鼠的改善作用及其潜在机制,将C57BL/6雌性小鼠分为6组,分别为空白对照组、模型对照组、多奈哌齐组（1.50 mg·kg^-1）以及抹茶低（0.75 g·kg^-1）、中（1.50 g·kg^-1）、高（3.00 g·kg^-1）剂量组。给样第31~35天,采用东莨菪碱（5 mg·kg^-1）建立记忆获得障碍模型。通过Morris水迷宫、跳台和避暗实验及HE染色、酶联免疫吸附法、蛋白质印迹等方法和技术进行分析。结果表明,抹茶在一定程度上可以改善记忆获得障碍模型小鼠的学习记忆能力,抑制海马组织中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达,增强海马组织中超氧化物歧化酶（SOD）、降低丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSH-Px）活性,并上调海马组织全细胞内的Nrf2、SOD、GSH-ST和HO-1蛋白的表达。同时调节胆碱代谢,增加海马胆碱含量,从而改善东莨菪碱诱导的小鼠认知功能损伤。综上,抹茶在改善东莨菪碱诱导的记忆障碍方面具有积极的作用,且其机制可能与增强抗炎活性、减轻氧化应激、调节神经递质有关。研究将丰富和完善抹茶改善阿尔茨海默病的作用机制,为抹茶相关保健食品开发提供研究基础和理论支撑。

关键词: 抹茶, 记忆获得障碍模型, 氧化应激, 神经保护

Abstract: To investigate the ameliorative effect of matcha on the memory acquisition impairment model mice induced by scopolamine and its underlying mechanisms, C57BL/6 female mice were divided into 6 groups, namely the control group, the scopolamine group, the donepezil group (1.50 mg·kg^-1), the low-dose matcha group (0.75 g·kg^-1), the medium-dose matcha group (1.50 g·kg^-1) and the high-dose matcha group (3.00 g·kg^-1). On the 31st to 35th days after the injection, the memory acquisition impairment model was established by using scopolamine (5.00 mg·kg^-1). The analysis was carried out through methods and techniques such as the Morris water maze test, the step-down test, the dark avoidance experiment, HE staining, enzyme-linked immunosorbent assay (ELISA) and Western blotting. The results demonstrate that matcha could, to a certain extent, improve the learning and memory abilities of the mice with memory acquisition impairment, inhibit the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the hippocampus tissue, enhance superoxide dismutase (SOD) in the hippocampus tissue, reduce malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity, and upregulate the expressions of Nrf2, SOD, GSH-ST and HO-1 proteins in hippocampal tissue. Meanwhile, it regulated choline metabolism and increased hippocampal choline content, thereby improving the cognitive dysfunction induced by scopolamine in mice. In conclusion, Guizhou-produced matcha plays a positive role in ameliorating memory impairment induced by scopolamine, and its mechanism may be related to enhancing anti-inflammatory activity, alleviating oxidative stress, and regulating neurotransmitters. This study enriched and improved the mechanism of matcha in ameliorating Alzheimer's disease and provided a research foundation and theoretical support for matcha-related health foods.

Key words: matcha, memory acquisition impairment model, oxidative stress, neuroprotection

中图分类号:

梁增彩, 马彩云, 郭俣, 朱翔, 陆涛峰, 陈亮, 李倩. 抹茶对记忆获得障碍模型小鼠的改善及机制分析[J]. 茶叶科学, 2025, 45(3): 497-508.

LIANG Zengcai, MA Caiyun, GUO Yu, ZHU Xiang, LU Taofeng, CHEN Liang, LI Qian. Improvement and Mechanism Analysis of Matcha on Mouse Model of Memory Acquisition Impairment[J]. Journal of Tea Science, 2025, 45(3): 497-508.

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