茶树WCOR413基因家族鉴定与表达调控分析

摘要/Abstract

摘要： 低温胁迫严重影响茶树的生长发育,以及茶叶的产量与品质。WCOR413基因家族在植物抗寒过程中发挥重要作用,然而茶树中该家族尚未得到系统鉴定。基于茶树泛基因组和冷驯化转录组数据,结合生物信息学及实时荧光定量PCR（RT-qPCR）分析,对茶树WCOR413（CsWCOR413）基因家族成员进行全面鉴定。结果表明,该家族包含6个成员（CsWCOR413-1~CsWCOR413-6）,根据蛋白质的保守结构域可分为质膜定位和内膜/类囊体膜定位两类。启动子元件分析发现,该家族基因可能受到多种转录因子的调控,从而灵敏地响应低温、脱水及脱落酸（ABA）等信号。RT-qPCR分析明确了CsWCOR413基因家族的表达模式,其中CsWCOR413-1主要在叶片中表达,响应茉莉酸（JA）和ABA信号,且受低温胁迫显著诱导;CsWCOR413-5则响应短时低温胁迫和钙离子信号。冷驯化转录组及加权基因共表达网络分析（Weighted correlation network analysis,WGCNA）表明,CsWCOR413-1的表达在自然冷驯化期间显著上调,且与低温信号响应核心转录因子CsCBF3存在高度共表达关系。亚细胞定位试验发现,CsWCOR413-1定位于细胞膜和细胞质中。通过双萤光素酶试验,明确了CsCBF1和CsCBF3可直接结合CsWCOR413-1的启动子并激活其表达。本研究系统阐明了CsWCOR413基因家族的特性及表达模式,揭示了CsWCOR413-1受CsCBF1和CsCBF3转录调控的分子机制,表明该基因可能在茶树响应低温胁迫中发挥重要作用。

关键词: 茶树, WCOR413基因家族, 低温胁迫, 泛基因组, 表达调控

Abstract: Low temperature stress significantly impairs the growth and development of tea plants (Camellia sinensis), as well as the yield and quality of tea. The WCOR413 gene family plays an important role in plant cold resistance. However, it has not yet been systematically identified in tea plants. This study employed pan-genome and cold acclimation transcriptome data of tea plants, combined with bioinformatics and RT-qPCR analysis, to identify members of the CsWCOR413 gene family. The results show that the CsWCOR413 family consists of six members (CsWCOR413-1 to CsWCOR413-6), which can be divided into two subclasses based on the conserved domains of the proteins: plasma membrane-localized and internal/thylakoid membrane-localized. Promoter cis-element analysis finds that the CsWCOR413 genes may be regulated by various transcription factors, enabling them to respond to cold, dehydration and ABA signals. RT-qPCR analysis reveals the expression patterns of the CsWCOR413 genes. CsWCOR413-1 was predominantly expressed in leaves, responded to JA and ABA signaling, and was significantly induced by low temperature stress. CsWCOR413-5 responded to short-term cold stress and calcium ion signaling. The transcriptome and Weighted gene co-expression network analysis (WGCNA) indicate that CsWCOR413-1 was significantly upregulated during natural cold acclimation and was strongly co-expressed with CsCBF3, a core transcription factor in cold signaling. Subcellular localization reveals that CsWCOR413-1 was localized in the cell membrane and cytoplasm. Dual-luciferase reporter assay confirms that CsCBF1 and CsCBF3 directly bound to the CsWCOR413-1 promoter and activated its expression. This study systematically elucidated the characteristics and expression profiles of the CsWCOR413 gene family and revealed that CsWCOR413-1 is transcriptionally regulated by CsCBF1 and CsCBF3, suggesting the crucial role of CsWCOR413-1 in tea plant under low temperature stress.

Key words: tea plants, WCOR413 gene family, low temperature stress, pan-genome, expression regulation

中图分类号:

S571.1
Q52

刘恩贝, 吴叶蝶, 徐苗苗, 凌名杏, 彭靖, 王洁, 王新超, 王璐. 茶树WCOR413基因家族鉴定与表达调控分析[J]. 茶叶科学, 2026, 46(1): 1-19.

LIU Enbei, WU Yedie, XU Miaomiao, LING Mingxing, PENG Jing, WANG Jie, WANG Xinchao, WANG Lu. Identification and Expression Regulation Analysis of the CsWCOR413 Gene Family in Camellia sinensis[J]. Journal of Tea Science, 2026, 46(1): 1-19.

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