茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究

doi:10.13305/j.cnki.jts.2013.05.008

茶叶科学 ›› 2013, Vol. 33 ›› Issue (5): 429-435.doi: 10.13305/j.cnki.jts.2013.05.008

茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究

虞希冲^1,2, 杨伟³, 吴波拉²

1. 浙江中医药大学药学院中药药理教研室浙江杭州 310053;
2. 温州医学院药学院药理学教研室,浙江温州 325053;
3. 温州医学院药学院药剂学教研室,浙江温州 325053

收稿日期:2013-02-20 修回日期:2013-06-22 出版日期:2013-10-30 发布日期:2019-09-04
作者简介:虞希冲（1975— ）,男,硕士,浙江温州人,主要从事神经药理学研究。E-mail: wenzhyu2004@yahoo.com
基金资助:
温州市科技计划项目：S20100044、S20100049

Theanine Depressed the Food Intake and Gastric Emptying in Female Mice via Lateral Hypothalamic AMPA and NMDA Receptor

YU Xi-chong^1,2, YANG Wei³, WU Bo-la²

1. Department of pharmacology of traditional Chinese medicine, Zhejiang Chinese medical university, Hangzhou 310053, China;
2. Department of pharmacology, Wenzhou medical college, Wenzhou 325035, China;
3. Department of pharmacy, Wenzhou medical college, Wenzhou 325035, China

Received:2013-02-20 Revised:2013-06-22 Online:2013-10-30 Published:2019-09-04

摘要/Abstract

摘要： 采用比色法观察脑室、核团内微注射和腹腔注射茶氨酸对外周胃排空的影响。结果表明,腹腔给予茶氨酸3~30βmg/kg后显著抑制摄食量和胃排空;脑室给药3~100βng后,对胃排空的影响表现出V型曲线,3~30βng茶氨酸剂量依赖性抑制胃排空,50、100βng茶氨酸使胃排空恢复到正常水平。然而,腹腔注射同样量的茶氨酸并无抑制作用。在下丘脑外侧核内注射同量茶氨酸,出现与脑室内类似的抑制胃排空作用,在弓状核、下丘脑腹内侧核内注射却无明显的改变。在下丘脑外侧核内注射NMDA和AMPA后均能诱导摄食和胃排空的增加,而茶氨酸10、30、100βng能抑制两者诱导的胃排空及NMDA诱导的摄食,茶氨酸3~100βng能抑制AMPA诱导的摄食。上述结果表明茶氨酸抑制摄食和胃排空作用可能与抑制下丘脑外侧核的NMDA受体和AMPA受体有关。

关键词: 茶氨酸, 下丘脑腹外侧核, 摄食, 胃排空, NDMA, AMPA

Abstract: In the present study, the food intake and gastric emptying of female mice were evaluated after theanine microinjection in cerebral ventrile, lateral hypothalamus, arcurate nuleius and ventromedial hypothalamic nucleus. Results showed that theanine 3~30βmg/kg intraperitoneal injection decreased food intake and gastric emptying; theanine 3~100βng microinjection into cerebral ventrile induced “V” style effects on gastric, theanine 3~30βng decreased gastric emptying dose-dependently while theanine 50 and 100βng recovered gastric emptying. Theanine microinjection in lateral hypothalamus displayed similar effects on gastic emptying as theanine i.c.v while microinjection in arcurate nucleus and ventromedial hypothalamic nucleus did not alert gastric emptying. Theanine decreased food intake and gastric emptying induced by NMDA and AMPA microinjection in lateral hypothalamus. It

Key words: theanine, lateral hypothalamus, food intake, gastric emptying, NMDA, AMPA

中图分类号:

Q517

虞希冲, 杨伟, 吴波拉. 茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究[J]. 茶叶科学, 2013, 33(5): 429-435. doi: 10.13305/j.cnki.jts.2013.05.008.

YU Xi-chong, YANG Wei, WU Bo-la. Theanine Depressed the Food Intake and Gastric Emptying in Female Mice via Lateral Hypothalamic AMPA and NMDA Receptor[J]. Journal of Tea Science, 2013, 33(5): 429-435. doi: 10.13305/j.cnki.jts.2013.05.008.

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茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究

Theanine Depressed the Food Intake and Gastric Emptying in Female Mice via Lateral Hypothalamic AMPA and NMDA Receptor

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