茶树CsPDAT1基因克隆及抗旱性分析

摘要/Abstract

摘要： 在茶树（Camellia sinensis）的生长发育过程中,干旱、盐害等非生物胁迫频繁发生,对茶叶的产量和品质造成了显著不利影响。聚焦于茶树磷脂：二酰甘油酰基转移酶1基因（CsPDAT1）,通过生物信息学分析、基因表达模式检测以及转基因功能验证等手段,系统探究了该基因在非生物胁迫响应中的功能。生物信息学分析表明,CsPDAT1编码的蛋白具有典型的溶血磷脂酰基转移酶（lysoPLA）结构域。实时荧光定量PCR结果显示,CsPDAT1在干旱及高盐处理下表达量显著上调,尤其在干旱胁迫初期表现明显。为深入解析其功能,构建了CsPDAT1过表达载体并转化拟南芥,成功获得3个纯合的T3代转基因株系。表型分析发现,在干旱胁迫下,过表达CsPDAT1的拟南芥植株种子萌发率显著高于野生型,丙二醛（MDA）含量降低,脯氨酸含量增加,且抗氧化酶活性显著增强。本研究证实CsPDAT1通过调控细胞膜脂代谢和渗透调节物质积累,增强植株对非生物胁迫的耐受性,为茶树抗逆分子育种提供了理论基础和基因资源。

关键词: 茶树, CsPDAT1, 基因克隆, 抗旱性

Abstract: During the growth and development of tea plants (Camellia sinensis), abiotic stresses such as drought and salinity frequently occur, causing significant adverse effects on tea yield and quality. This study focused on the tea phospholipid: diacylglycerol acyltransferase 1 (PDAT1) gene and systematically investigated its function in response to abiotic stresses through bioinformatics analysis, gene expression pattern detection, and transgenic functional verification. Bioinformatics analysis reveals that the protein encoded by the CsPDAT1 gene possesses a typical lysophospholipid acyltransferase (lysoPLA) domain. Real-time quantitative PCR (qRT-PCR) results demonstrats that the expression of the CsPDAT1 gene was significantly upregulated under drought and high-salt treatments, particularly during the early stages of drought stress. To further elucidate its function, researchers constructed an overexpression vector for the CsPDAT1 gene and transformed it into Arabidopsis thaliana. Finally three homozygous T3-generation transgenic lines were obtained. Phenotypic analysis reveals that under drought stress, Arabidopsis plants overexpressing the CsPDAT1 gene exhibited a significantly higher seed germination rate compared to that of wild-type plants, along with reduced malondialdehyde (MDA) content, increased proline content, and significantly enhanced antioxidant enzyme activity. This study confirmed that the CsPDAT1 gene in tea plants enhances plant tolerance to abiotic stress by regulating cell membrane lipid metabolism and the accumulation of osmoprotectants, providing a theoretical foundation and genetic resources for stress-resistant molecular breeding in tea plants.

Key words: tea plant, CsPDAT1, gene cloning, drought resistance

中图分类号:

沈英姿, 李朵姣, 胡新荣, 江丽, 郑寨生, 留惠康, 袁名安. 茶树CsPDAT1基因克隆及抗旱性分析[J]. 茶叶科学, 2025, 45(6): 920-930.

SHEN Yingzi, LI Duojiao, HU Xingrong, JIANG Li, ZHENG Zhaisheng, LIU Huikang, YUAN Ming′an. Cloning of the CsPDAT1 Gene from Camellia sinensis and Its Role in Drought Tolerance[J]. Journal of Tea Science, 2025, 45(6): 920-930.

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