两种工艺红茶粉的功能成分差异及其对小鼠结肠炎改善效应的研究

茶叶科学 ›› 2026, Vol. 46 ›› Issue (1): 122-138.

两种工艺红茶粉的功能成分差异及其对小鼠结肠炎改善效应的研究

吕梦绮¹, 陈薇¹, 顾大江², 谢伟³, 袁懿炜¹, 徐倩倩¹, 闻正阳¹, 赵进^1,*

1.中国计量大学生命科学学院,食品营养与质量安全研究所,浙江杭州 310018;
2.易晓食品（衢州）有限公司,浙江开化,324300;
3.杭州七香茶业有限公司,浙江杭州 311606

收稿日期:2025-07-23 修回日期:2025-10-13 出版日期:2026-02-15 发布日期:2026-02-06
通讯作者: * zhaojin@cjlu.edu.cn
作者简介:吕梦绮,女,硕士研究生,研究方向为药食同源植物功效评价研究。
基金资助:
衢州市重点科技攻关竞争性项目（2023k098）; 浙江省团队科技特派员项目（2025TDT001）; 建德市农业与社会发展计划项目（2024NS10）

Comparative Study on Functional Components of Two Types of Black Tea Powder and Their Ameliorative Effects on Colitis in Mice

LÜ Mengqi¹, CHEN Wei¹, GU Dajiang², XIE Wei³, YUAN Yiwei¹, XU Qianqian¹, WEN Zhengyang¹, ZHAO Jin^1,*

1. Institute of Food Nutrition and Quality Safety, College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
2. Yixiao Food (Quzhou) Co., LTD., Kaihua 324300, China;
3. Hangzhou Qixiang Tea Industry Co., LTD., Hangzhou 311606, China

Received:2025-07-23 Revised:2025-10-13 Online:2026-02-15 Published:2026-02-06

摘要/Abstract

摘要： 分析两种工艺制备的红茶粉的主要功能成分含量变化和成品特征香气成分,并将50只8周龄C57BL/6J小鼠随机分为正常对照组（NC）、葡聚糖硫酸钠（DSS）结肠炎模型组（DSS）、EGCG阳性干预组（EGCG,剂量为20 mg·kg^-1）、速溶红茶粉干预组（SU,剂量为300 mg·kg^-1）与转溶红茶粉干预组（ZHUAN,剂量为300 mg·kg^-1）,探究红茶粉对DSS诱导的小鼠结肠炎的改善效应。结果表明,两种红茶粉中游离氨基酸、可溶性蛋白、茶多酚、咖啡碱含量差异显著,两者均检测出芳樟醇、叶醇、α-松油醇、水杨酸甲酯及香叶醇等特征性挥发物成分;两种红茶粉均能显著缓解DSS诱导的小鼠结肠炎症状,主要表现为减轻小鼠体质量的下降、抑制结肠缩短、降低血清和结肠组织中促炎因子（TNF-α、IL-1β、IL-6）水平,能降低MDA含量、提高SOD活性,明显缓解结肠组织氧化应激损伤症状,并且紧密连接蛋白ZO-1、Occludin表达量增加、血清LPS水平降低,说明改善了肠道屏障的通透性。研究结果揭示,速溶红茶粉与转溶红茶粉对DSS诱导的小鼠结肠炎均具有改善作用。

关键词: 红茶粉, 速溶红茶, 转溶红茶, 结肠炎, 肠道通透性

Abstract: This study aimed to analyze variations in the major functional components and characteristic aroma profiles of black tea powders prepared through two distinct processing techniques, and further investigate their protective effects against dextran sulfate sodium (DSS)-induced colitis in mice. Fifty 8-week-old C57BL/6J mice were randomly assigned to five groups: normal control (NC), DSS-induced colitis model (DSS), EGCG positive intervention (EGCG, 20 mg·kg^-1), instant black tea powder intervention (SU, 300 mg·kg^-1) and trans-solubilized black tea powder intervention (ZHUAN, 300 mg·kg^-1). The results demonstrate that: (1) The contents of free amino acids, soluble proteins, tea polyphenols and caffeine underwent significant alterations during processing due to methodological differences. (2) Characteristic volatile compounds (including linalool, leaf alcohol, α-terpineol, methyl salicylate, and geraniol) were commonly detected in both tea powders. (3) Both tea powders significantly alleviated DSS-induced colitis symptoms, as evidenced by reduced body weight loss and colon shortening, decreased pro-inflammatory cytokine (TNF-α, IL-1β and IL-6) levels in serum and colon tissue, mitigated oxidative stress injury (decreased MDA content and elevated SOD activity), and improved intestinal barrier permeability (up regulated expressions of tight junction proteins ZO-1 and Occludin, and decreased serum LPS levels). These findings indicate that instant and trans-solubilized black tea powders exhibit protective effects against DSS-induced murine colitis.

Key words: black tea powder, instant black tea, trans-solubilized black tea, colitis, intestinal permeability

中图分类号:

吕梦绮, 陈薇, 顾大江, 谢伟, 袁懿炜, 徐倩倩, 闻正阳, 赵进. 两种工艺红茶粉的功能成分差异及其对小鼠结肠炎改善效应的研究[J]. 茶叶科学, 2026, 46(1): 122-138.

LÜ Mengqi, CHEN Wei, GU Dajiang, XIE Wei, YUAN Yiwei, XU Qianqian, WEN Zhengyang, ZHAO Jin. Comparative Study on Functional Components of Two Types of Black Tea Powder and Their Ameliorative Effects on Colitis in Mice[J]. Journal of Tea Science, 2026, 46(1): 122-138.

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