广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究

茶叶科学 ›› 2026, Vol. 46 ›› Issue (1): 139-150.

广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究

安鲁敬^1,2, 钱益跃³, 常可^1,2, 田宝明², 穆丹¹, 张明珠^1,*, 陈红平², 张相春^2,*

1.皖西南生物多样性研究与生态保护安徽省重点实验室,安庆师范大学生命科学学院,安徽安庆 246133;
2.茶树种质创新与资源利用全国重点实验室,中国农业科学院茶叶研究所,浙江杭州 310008;
3.浙江省金华生态环境监测中心,浙江金华 321000

收稿日期:2025-10-15 修回日期:2025-11-28 发布日期:2026-02-06
通讯作者: * Echo8453@163.com;zhangxc@tricaas.com
作者简介:安鲁敬,男,硕士研究生,主要从事茶精深加工健康利用方面的研究。
基金资助:
四川省重点研发计划（2024YFHZ0179）; 安徽省新时代育人质量工程项目（研究生教育）教学研究一般项目（2023jyjxggyjY194）; 安徽省自然科学基金青年项目（2308085QC78）

Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities

AN Lujing^1,2, QIAN Yiyue³, CHANG Ke^1,2, TIAN Baoming², MU Dan¹, ZHANG Mingzhu^1,*, CHEN Hongping², ZHANG Xiangchun^2,*

1. The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, School of Life Sciences, Anqing Normal University, Anqing 246133, China;
2. State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
3. Zhejiang Jinhua Ecological and Environmental Monitoring Center, Jinhua 321000, China

Received:2025-10-15 Revised:2025-11-28 Published:2026-02-06

摘要/Abstract

摘要： 目前关于直接以茶浸提液为原料构建新型功能复合物研究较少,本研究以广元黄茶茶汤和硫酸锌为原料,通过绿色简便的一步自组装法成功制备了广元黄茶-锌纳米复合物（Y-Zn）。通过紫外-可见分光光度计（UV-vis）、透射电子显微镜（TEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）表征显示,所合成的纳米复合物粒径均一、分散性良好。抗菌试验表明,Y-Zn对包括耐药菌株在内的多种革兰氏阳性菌表现出剂量依赖性的抗菌效果,并能有效抑制细菌生物膜形成;抗氧化试验结果显示,Y-Zn具备优异的自由基清除能力,在20 μg·mL^-1质量浓度下清除率超过80%。细胞试验证实,Y-Zn通过下调诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达,抑制脂多糖（LPS）诱导的巨噬细胞炎症反应,且斑马鱼模型显示其生物安全性良好。本研究直接以茶汤为对象开发出一种兼具多种生物活性和安全性的自组装复合物,为我国丰富茶叶资源在生命健康领域中的应用提供了思路。

关键词: 茶汤, 自组装, 纳米复合物, 抗菌活性, 抗炎活性

Abstract: Currently, few studies have reported on the direct use of tea extracts as raw materials for creating novel functional composites. This study successfully synthesized Guangyuan Yellow Tea-Zinc Nanocomposites (Y-Zn) using Guangyuan Yellow Tea infusion and zinc sulfate as raw materials through a green, simple one-step self-assembly method. Characterization via UV-vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) reveals that the synthesized nanocomposite exhibits uniform particle size and excellent dispersion. Antibacterial experiments demonstrated that Y-Zn exhibits dose-dependent antibacterial effects against multiple Gram-positive bacteria, including drug-resistant strains, and effectively inhibits bacterial biofilm formation. Antioxidant experiments revealed its excellent free radical scavenging ability, achieving over 80% scavenging efficiency at a concentration of 20 μg·mL^-1. Cellular experiments confirmed that Y-Zn suppresses lipopolysaccharide (LPS)-induced macrophage inflammatory responses by downregulating inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, zebrafish models demonstrated its favorable biosafety profile. In summary, this study directly utilized tea infusion as the subject to develop a self-assembled composite that possesses multiple biological activities and safety, providing insights into the application of China's abundant tea resources in the field of life sciences and health.

Key words: tea infusion, self-assembly, nanocomposite, antibacterial activity, anti-inflammatory activity

中图分类号:

S571.1
TQ460

安鲁敬, 钱益跃, 常可, 田宝明, 穆丹, 张明珠, 陈红平, 张相春. 广元黄茶自组装锌复合物的制备及其抗菌抗炎活性研究[J]. 茶叶科学, 2026, 46(1): 139-150.

AN Lujing, QIAN Yiyue, CHANG Ke, TIAN Baoming, MU Dan, ZHANG Mingzhu, CHEN Hongping, ZHANG Xiangchun. Preparation of Guangyuan Yellow Tea Self-Assembled Zinc Nanocomposite and Their Antibacterial and Anti-Inflammatory Activities[J]. Journal of Tea Science, 2026, 46(1): 139-150.

参考文献

[1] 姜仁华, 陈富桥, 潘昌健, 等. 关于发展茶业新质生产力的思考[J]. 中国茶叶, 2024, 46(11): 1-6.
Jiang R H, Chen F Q, Pan C J, et al.Thoughts on developing new quality productivity in the tea industry[J]. China Tea, 2024, 46(11): 1-6.
[2] 梅宇. 2024年中国茶叶生产与内销形势分析[J]. 中国茶叶, 2025, 47(6): 24-30.
Mei Y.Analysis of China's tea production and domestic sales in 2024[J]. China Tea, 2025, 47(6): 24-30.
[3] 杨亚军. 坚持问题导向推动我国茶业高质量发展—我国茶产业发展现状与建议[J]. 中国茶叶, 2023, 45(1): 1-5.
Yang Y J.Adhere to problem orientation and promote high-quality development of China's tea industry: current situation and suggestions of tea industry development in China[J]. China Tea, 2023, 45(1): 1-5.
[4] 尹军峰. 中国茶饮创新发展态势与启示[J]. 中国茶叶, 2025, 47(3): 1-5.
Yin J F.The innovative development trend and enlightenment of China's tea drinking[J]. China Tea, 2025, 47(3): 1-5.
[5] 俞蓉欣, 郑芹芹, 陈红平, 等. 儿茶素生物医用纳米材料研究进展[J]. 茶叶科学, 2022, 42(4): 447-462.
Yu R X, Zheng Q Q, Chen H P, et al.Recent advances in catechin biomedical nanomaterials[J]. Journal of Tea Science, 2022, 42(4): 447-462.
[6] Zhang Y X, Feng X Y, Lin H Y, et al.Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota[J]. Food & Function, 2022, 13(24): 13040-13051.
[7] Yang M C, Zhou L, Kan Z P, et al.Beneficial health effects and possible health concerns of tea consumption: a review[J]. Beverage Plant Research, 2025, 5: e035. doi: 10.48130/bpr-0025-0036.
[8] 徐伟, 俞蓉欣, 张相春, 等. 多酚自组装抗菌生物材料的构建及其应用进展[J]. 茶叶科学, 2024, 44(1): 1-15.
Xu W, Yu R X, Zhang X C, et al.Construction of polyphenol self-assembly antibacterial biomaterials and progress in their applications[J]. Journal of Tea Science, 2024, 44(1): 1-15.
[9] Yang M, Zhang X, Yang C S.Bioavailability of tea polyphenols: a key factor in understanding their mechanisms of action in vivo and health effects[J]. Journal of Agricultural and Food Chemistry, 2025, 73(7): 3816-3825.
[10] Mereles D, Hunstein W.Epigallocatechin-3-gallate (EGCG) for clinical trials: more pitfalls than promises?[J]. International Journal of Molecular Sciences, 2011, 12(9): 5592-5603.
[11] Xu C, Zhou S, Song H Z, et al.Green tea polyphenols-derived hybrid materials in manufacturing, environment, food and healthcare[J]. Nano Today, 2023, 52: 101990. doi: 10.1016/j.nantod.2023.101990.
[12] Xiang X J, Feng X, Lu S J, et al.Indocyanine green potentiated paclitaxel nanoprodrugs for imaging and chemotherapy[J]. Exploration, 2022, 2(4): 20220008. doi: 10.1002/EXP.20220008.
[13] Wang Y L, Mu Y, Zhang Y L, et al.Accessible and effective nanomedicines: self-assembly products from Chinese herbal medicines (CHMs)[J]. Advanced Functional Materials, 2025, 35(9): 2416151. doi: 10.1002/adfm.202416151.
[14] Wu J J, Yang Y, Yuan X Y, et al.Role of particle aggregates in herbal medicine decoction showing they are not useless: considering Coptis chinensis decoction as an example[J]. Food Function, 2020, 11(12): 10480-10492.
[15] Zhou J W, Liu J, Lin D, et al.Boiling-induced nanoparticles and their constitutive proteins from Isatis indigotica Fort. root decoction: purification and identification[J]. Journal of Traditional and Complementary Medicine, 2017, 7(2): 178-187.
[16] Lü S W, Su H, Sun S, et al.Isolation and characterization of nanometre aggregates from a Bai-Hu-Tang decoction and their antipyretic effect[J]. Scientific Reports, 2018, 8(1): 12209. doi: 10.1038/s41598-018-30690-5.
[17] Lin D, Du Q, Wang H Q, et al.Antidiabetic micro-/nanoaggregates from Ge-Gen-Qin-Lian-Tang decoction increase absorption of baicalin and cellular antioxidant activity in vitro[J]. BioMed Research International, 2017, 2017: 9217912. doi: 10.1155/2017/9217912.
[18] Zhou J W, Gao G Z, Chu Q P, et al.Chromatographic isolation of nanoparticles from Ma-Xing-Shi-Gan-Tang decoction and their characterization[J]. Journal of Ethnopharmacology, 2014, 151(3): 1116-1123.
[19] Zhou J W, Zhang J, Gao G Z, et al.Boiling licorice produces self-assembled protein nanoparticles: a novel source of bioactive nanomaterials[J]. Journal of Agricultural and Food Chemistry, 2019, 67(33): 9354-9361.
[20] Zhang Y, Cui Z, Mei H, et al.Angelica sinensis polysaccharide nanoparticles as a targeted drug delivery system for enhanced therapy of liver cancer[J]. Carbohydrate Polymers, 2019, 219: 143-154. doi: 10.1016/j.carbpol.2019.04.041.
[21] Li T, Wang P L, Guo W B, et al.Natural berberine-based Chinese herb medicine assembled nanostructures with modified antibacterial application[J]. ACS Nano, 2019, 13(6): 6770-6781.
[22] Dai L, Zhu W Y, Si C L, et al.“Nano-Ginseng” for enhanced cytotoxicity against cancer cells[J]. International Journal of Molecular Sciences, 2018, 19(2): 627. doi: 10.3390/ijms19020627.
[23] Li J M, Zhang Y L, Jin T, et al.Advanced pharmaceutical nanotechnologies applied for Chinese herbal medicines[J]. Advanced Science, 2025, 12(31): e00167. doi: 10.1002/advs.202500167.
[24] Yadi M, Mostafavi E, Saleh B, et al.Current developments in green synthesis of metallic nanoparticles using plant extracts: a review[J]. Artificial Cells, Nanomedicine, and Biotechnology, 2018, 46(s3): S336-S343.
[25] Wang S S, Wang Z Q, Li Z G, et al.Recent advances in tea and other plant polyphenol biomaterials for antibacterial and disease treatment[J]. Beverage Plant Research, 2025, 5: e010. doi: 10.48130/bpr-0025-0012.
[26] Du Y J, Huo Y, Yang Q, et al.Ultrasmall iron-gallic acid coordination polymer nanodots with antioxidative neuroprotection for PET/MR imaging-guided ischemia stroke therapy[J]. Exploration, 2023, 3(1): 20220041. doi: 10.1002/EXP.20220041.
[27] Yu R X, Chen H P, He J, et al.Engineering antimicrobial metal-phenolic network nanoparticles with high biocompatibility for wound healing[J]. Advanced Materials, 2024, 36(6): e2307680. doi: 10.1002/adma.202307680.
[28] Ye Y, Zheng Q Q, Wang Z Q, et al.Metal-phenolic nanoparticles enhance low temperature photothermal therapy for bacterial biofilm in superficial infections[J]. Journal of Nanobiotechnology, 2024, 22: 713. doi: 10.1186/s12951-024-02985-5.
[29] Xu W, Jia X Y, Yang M C, et al.Tea polyphenol self-assembly nanocomposite coating for fruit preservation[J]. ACS Nano, 2025, 19(31): 28146-28159.
[30] Jeejeebhoy K.Zinc: an essential trace element for parenteral nutrition[J]. Gastroenterology, 2009, 137(s5): S7-S12.
[31] Biesinger M C, Payne B P, Grosvenor A P, et al.Resolving surface chemical states in XPS analysis of first row transition metals, oxides and hydroxides: Cr, Mn, Fe, Co and Ni[J]. Applied Surface Science, 2011, 257(7): 2717-2730.
[32] Beroz F, Yan J, Meir Y, et al.Verticalization of bacterial biofilms[J]. Nature Physics, 2018, 14(9): 954-960.
[33] Chong Z Z, Souayah N.Oxidative stress: pathological driver in chronic neurodegenerative diseases[J] 2025, 14(6): 696. doi: 10.3390/antiox14060696.
[34] Joorabloo A, Liu T Q.Recent advances in reactive oxygen species scavenging nanomaterials for wound healing[J]. Exploration, 2024, 4(3): 20230066. doi: 10.1002/EXP.20230066.
[35] Peng H B, Yao F B, Zhao J X, et al.Unraveling mitochondria-targeting reactive oxygen species modulation and their implementations in cancer therapy by nanomaterials[J]. Exploration, 2023, 3(2): 20220115. doi: 10.1002/EXP.20220115.
[36] Takatsuka M, Goto S, Kobayashi K, et al.Evaluation of pure antioxidative capacity of antioxidants: ESR spectroscopy of stable radicals by DPPH and ABTS assays with singular value decomposition[J]. Food Bioscience, 2022, 48: 101714. doi: 10.1016/j.fbio.2022.101714.
[37] 时杰, 王永安, 孙基泽, 等. 亚硒酸钠通过活性氧(ROS)/谷胱甘肽(GSH)/谷胱甘肽过氧化物酶4(GPX4)轴诱导非小细胞肺癌A549细胞铁死亡[J]. 中国无机分析化学, 2024, 14(1): 124-130.
Shi J, Wang Y A, Sun J Z, et al.Sodium selenite induces ferroptosis in non-small cell lung cancer A549 cells via reactive oxygen species(ROS)/glutathione(GSH)/glutathione peroxidase 4(GPX4) axis[J]. Chinese Journal of Inorganic Analytical Chemistry, 2024, 14(1): 124-130.
[38] Lo J, Liu C C, Li Y S, et al.Punicalagin attenuates LPS-Induced Inflammation and ROS production in microglia by inhibiting the MAPK/NF-κB signaling pathway and NLRP3 inflammasome activation[J]. Journal of Inflammation Research, 2022, 15: 5347-5359. doi: 10.2147/JIR.S372773.
[39] Meng X Q, Wei Q, Wang S Y, et al.Anti-inflammatory effect of polysaccharides from Sambucus williamsii Hance roots in lipopolysaccharide-stimulated RAW264.7 macrophages and acute lung injury in mice[J]. International Journal of Biological Macromolecules, 2025, 306(Part1): 141368. doi: 10.1016/j.ijbiomac.2025.141368.
[40] Akhtar M, Rafique H, Alam Y, et al.Pectin (RG-1)-like polysaccharides isolated from Gastrodiae rhizoma via fractional ethanol precipitation: potent inhibitors of pro inflammatory enzyme modulation targeting iNOS and COX-2[J]. International Journal of Biological Macromolecules, 2025, 322(Part4): 146784. doi: 10.1016/j.ijbiomac.2025.146784.