Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice

PIAO Zai-ri; Wang Yue-fei; YANG Xian-qiang; CHEN Liu-ji; YANG Yong; ZHAO Bao-lu; JIAO Hong-li; ZHOU Dang-yi

doi:10.13305/j.cnki.jts.2003.02.007

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Journal of Tea Science ›› 2003, Vol. 23 ›› Issue (2) : 119-123. DOI: 10.13305/j.cnki.jts.2003.02.007

Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice

PIAO Zai-ri¹, Wang Yue-fei¹, YANG Xian-qiang¹, CHEN Liu-ji¹, YANG Yong¹, ZHAO Bao-lu², JIAO Hong-li², ZHOU Dang-yi³

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Abstract

The effects of tea catechins on lead-induced lipid peroxidation damage in HepG2 cells and tea polyhenol on the content of lead in lead-exposed mice were investigated.HepG2 cell viability was determined using the MTT method,lipid peroxidation was assayed by detetmining the production rate of thibarbiturie acid reactive substance(TBARS)which was expressed as malondialdehyde(MDA)equivalents.The content of lead in lead-exposed mice was determined using hydride atomic fluorescence spectroscopic method. Results showed that tea catechins had protective effects on lead-exposed HepG2 cell viability and inhibitory effects on TBARS formation in HepG2 cells.All the indices of the mice in the exposed groups were significantly higher than those in the control.Tea polyphenol was able to accelerate the removal of lead from liver,but the same effects were not found in blood、kidney, brain and thigbones.

Key words

Lead exposure / Lipid preoxidation / Removal of lead / Tea catechins / Tea polyphenol / Tea science

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PIAO Zai-ri, Wang Yue-fei, YANG Xian-qiang, CHEN Liu-ji, YANG Yong, ZHAO Bao-lu, JIAO Hong-li, ZHOU Dang-yi. Effects of Tea Catechins on Lead-induced Lipid Peroxidation Damage in HepG2 Cells and Tea Polyphenol on the Content of Lead in Lead-exposed Mice[J]. Journal of Tea Science. 2003, 23(2): 119-123 https://doi.org/10.13305/j.cnki.jts.2003.02.007

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