Abstract: Eurotium cristatum isolated from brick tea were enlarged to foster by liquid fermentation and then crude enzyme was prepared from the culture of Eurotium cristatum. Cellulase activity of the crude enzyme liquid was measured by 3,5-dinitrosalicylic acid method under different conditions. The results showed that the optimum temperature for enzymatic reaction was 50℃, and the optimum reactive pH value was at 4.8. The cellulase activity was stabile under conditions of 30~50℃ and pH 4.0~6.0. The cellulase activity was stimulated by Ca²⁺、Mg²⁺ ions and inhibited by Mn²⁺、Zn²⁺、Pb²⁺ ions. The research will be significance for production of brick tea.

Key words: brick tea, Eurotium cristatum, cellulose, enzymatic property