Foliar spraying in different levels of Na₂SeO₃ and (CH₃COO)₂Zn·2H₂O was carried out to determine the effects of Se, Zn and their interaction on the chemical form of Se in tea leaves in spring season. The results showed that: (1) The content of Se in alcohol soluble, water soluble, salt soluble was increased when spraying Na₂SeO₃, particularly the content of Se of three kinds of chemical forms was increased significantly when the spraying concentration of Se was 200~400 μg/mL. Among there the proportion of the total alcohol soluble, Se was the highest and water soluble was higher than salt soluble, the proportion of the total of alcohol soluble and water soluble Se increased in varying degrees comparing with the control. (2)The cumulative effect of the content of Se of three kinds of chemical forms was obvious when the spraying concentration of Zn was between 0.20%~0.40%. The Se of three kinds of chemical forms assimilation was restrained when the zinc concentration increased to 1.2%. (3)The overall three kinds of forms of selenium levels were lower and there was no obvious difference of the proportion of the total of Se between alcohol soluble and water soluble when spraying Se at 50μg/mL and the different concentration of Zn, but both of them were higher than salt soluble Se. The distribution of chemical forms of selenium was similar to single Se treatment when the spraying with the other concentrations of Se and Zn. The content of Se of three kinds of chemical forms was increased obviously when the spraying of Se in 200~400 μg/mL and Zn in 0.4%~0.8%, it also raised the proportion of the total of alcohol soluble and water soluble Se comparing with the control, but the effect achieved the best when spraying Se in 200 μg/mL and Zn in 0.80%.