Research on Preparation and Antitumor Activity of EGCG Naonparticles

HUANG Meirong; YING Hao; JIANG Yongwen; JIANG Heyuan; DU Qizhen

doi:10.13305/j.cnki.jts.2015.06.015

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (6) : 605-612. DOI: 10.13305/j.cnki.jts.2015.06.015

Research on Preparation and Antitumor Activity of EGCG Naonparticles

HUANG Meirong¹, YING Hao¹, JIANG Yongwen², JIANG Heyuan², DU Qizhen^1,*

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Abstract

(-)-Epigallocatechin-3-gallate (EGCG) and ascorbic acid (Vc) were loaded in heat treated β-lactoglobulin (β-Lg) to prepare EGCG naonparticles. The effects of EGCG and Vc with different molar ratios on the particle size, Zeta potential, entrapment efficiency, loading capacity, the change of color were investigated. The antitumor activity and mechanism of EGCG naonparticles were obtained by MTT assay and cytokinesis block micronucleus test (CBMNT). The results showed that EGCG naonparticles had stronger inhibition on human melanoma cell (A-375) and esophageal carcinoma cell (TE-1), and significantly increased the number of micronuclei, necrosis and apoptosis of the A-375 and TE1-1 carcinoma cell.

Key words

β-lactoglobulin (β-Lg); / (-)-epigallocatechin-3-gallate (EGCG) / antitumor activity / nanoparticle / Vc

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HUANG Meirong, YING Hao, JIANG Yongwen, JIANG Heyuan, DU Qizhen. Research on Preparation and Antitumor Activity of EGCG Naonparticles[J]. Journal of Tea Science. 2015, 35(6): 605-612 https://doi.org/10.13305/j.cnki.jts.2015.06.015

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