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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (6): 605-612.doi: 10.13305/j.cnki.jts.2015.06.015

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Research on Preparation and Antitumor Activity of EGCG Naonparticles

HUANG Meirong1, YING Hao1, JIANG Yongwen2, JIANG Heyuan2, DU Qizhen1,*   

  1. 1. The Institute of Food and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;
    2. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2015-04-08 Revised:2015-06-24 Online:2015-12-15 Published:2019-08-26

Abstract: (-)-Epigallocatechin-3-gallate (EGCG) and ascorbic acid (Vc) were loaded in heat treated β-lactoglobulin (β-Lg) to prepare EGCG naonparticles. The effects of EGCG and Vc with different molar ratios on the particle size, Zeta potential, entrapment efficiency, loading capacity, the change of color were investigated. The antitumor activity and mechanism of EGCG naonparticles were obtained by MTT assay and cytokinesis block micronucleus test (CBMNT). The results showed that EGCG naonparticles had stronger inhibition on human melanoma cell (A-375) and esophageal carcinoma cell (TE-1), and significantly increased the number of micronuclei, necrosis and apoptosis of the A-375 and TE1-1 carcinoma cell.

Key words: (-)-epigallocatechin-3-gallate (EGCG), Vc, β-lactoglobulin (β-Lg);, nanoparticle, antitumor activity

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