Identification and Expression Analysis of Terpene Synthesis Related Genes during the Withering of White Tea

CHEN Xuejin; WANG Pengjie; LIN Xinying; GU Mengya; ZHENG Yucheng; ZHENG Zhilin; YE Naixing

doi:10.13305/j.cnki.jts.2020.03.007

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (3) : 363-374. DOI: 10.13305/j.cnki.jts.2020.03.007

Identification and Expression Analysis of Terpene Synthesis Related Genes during the Withering of White Tea

CHEN Xuejin, WANG Pengjie, LIN Xinying, GU Mengya, ZHENG Yucheng, ZHENG Zhilin, YE Naixing^*

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Abstract

Terpenes are the important secondary metabolites in plants and play an important role in the composition of the volatile aroma of tea plants. In this study, 141 tea plant terpenoid synthesis-related genes were identified from the tea plant genome database. Their expression specificities in different tissues were analyzed. Sixteen terpene synthetic genes which were highly expressed in the apical buds and young leaves of tea plants were screened. The results of bioinformatics methods show that the phylogenetic relationship divides the genes related to terpene synthesis of tea plant, Arabidopsis and grape into four subfamilies. The terpenoid synthesis related genes contain 5 to 14 exons and a large number of cis-related elements closely related to light response, plant growth and development, hormone and stress response according to the upstream promoter region analysis. Fluorescence quantitative detection showed that the expressions of CsMVK, CsDXS and CsGGPS were significantly up-regulated during the withering process of white tea. The expressions of CsDXR, CsMCT, CsCMK, CsMCS, CsHDS, CsGPPS and CsGGPPS showed the highest expressions at 4 h and 24 h after withering. The results of this study provided a theoretical basis for further exploring the functions of terpenoid synthesis related genes in tea.

Key words

aroma / expression analysis / terpenes / white tea

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CHEN Xuejin, WANG Pengjie, LIN Xinying, GU Mengya, ZHENG Yucheng, ZHENG Zhilin, YE Naixing. Identification and Expression Analysis of Terpene Synthesis Related Genes during the Withering of White Tea[J]. Journal of Tea Science. 2020, 40(3): 363-374 https://doi.org/10.13305/j.cnki.jts.2020.03.007

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