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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (4): 492-500.doi: 10.13305/j.cnki.jts.2020.04.005

• Research Paper • Previous Articles     Next Articles

A Preliminary Study on Determining Tea Plant Age Based on Anatomical Structure of Tea’s Stem

JIANG Yan1, LI Xinghui2, PU Dian3, LAN Zengquan4,*, WU Tian1,*   

  1. 1. Southwest Landscape Architecture Engineering Research Center of State Forestry Administration, Southwest Forestry University, Kunming 650224, China;
    2. Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    3. School of Ecology and Soil and Water Conservation, Southwest Forestry University, Kunming 650224, China;
    4. Tea Ecology Research Center, Southwest Green Development Institute, Southwest Forestry University, Kunming 650224, China
  • Received:2019-09-19 Revised:2019-10-30 Online:2020-08-15 Published:2020-08-18

Abstract: Tea plants were taken as the research objects in this study. Using tea stem slicing and dyeing technologies, the age of tea plants was accurately determined based on the digital images. Under ordinary light and fluorescence of bio-digital microscope, the untreated sections of tea stem, saffron-dyed sections and acridine orange-dyed sections of tea stem were compared and analyzed. The result shows that acridine orange dyeing was the best to determine the age of tea plants. The acridine orange dyeing method combined with digital image analysis had the characteristics of fast, accurate, simple and low cost. In conclusion, the technology also had a scientific reference significance for the identification of annual rings of living large and ancient tea plants.

Key words: stem slices of tea plant, dyeing technology, fluorescence, growth ring, digital image

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