Molecular Cloning and Quantitative Analysis of GDH, GS and GOGAT Genes from Leave of Tea Plant

LIN Zheng-he; ZHONG Qiu-sheng; CHEN Chang-song

doi:10.13305/j.cnki.jts.2012.06.014

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (6) : 523-529. DOI: 10.13305/j.cnki.jts.2012.06.014

Molecular Cloning and Quantitative Analysis of GDH, GS and GOGAT Genes from Leave of Tea Plant

LIN Zheng-he, ZHONG Qiu-sheng, CHEN Chang-song^*

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Abstract

This research has separated gene conservative regions of glutamate dehydrogenase (GDH), Glutamine synthetase (GS) and Glutamine oxoglutarate aminotransferase (GOGAT), which were key enzymes involving in the metabolism of amino acid in tea plant, from four tea germplasms. Sequences of GDH, GS and GOGAT conservative regions have been submitted to GeneBank and their accession number were JN602371, JN602372 and JN602373, respectively. Real-time quantitative PCR analysis revealed that GDH gene has higher transcription in 0314C (germplasm with relatively high amino acid content) than the other three germplasms, while lower transcription in 0212-15 (germplasm with relatively low amino acid content) comparing to the others. The transcription of GS and GOGAT gene were in apparently low level in 0314C and high level in 0212-15 and 0318D, respectively. Regression analysis indicated that the expression of GS gene was negatively related to the contents of theanine, lysine and lactamine, whereas the expression of GDH gene was positively related to the content of theanine.

Key words

clone / fluorescence quantitative PCR / gene / tea germplasm

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LIN Zheng-he, ZHONG Qiu-sheng, CHEN Chang-song. Molecular Cloning and Quantitative Analysis of GDH, GS and GOGAT Genes from Leave of Tea Plant[J]. Journal of Tea Science. 2012, 32(6): 523-529 https://doi.org/10.13305/j.cnki.jts.2012.06.014

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