Abstract: CTPS(Crude tea polysaccharide, CTPS) were extracted from coarse tea, CTPS were purified by DEAE-cellulose and Sephacryl S-300 column chromatography, there fractions of TPS were obtained, TPS2-H₂O, TPS2-0.25 and TPS2-0.40. The contents of neutral saccharide of them were 41.23%, 29.33% and 21.39% respectively, and the contents of uronic acid, 19.5%，22.5% and 56.4%, and the contents of protein, not detected, 0.8% and not detected. By means of GC, the monosaccharide composition and its molar ratio of the polysaccharides were confirmed, and they are rhamnose, arabinose, xylose, mannose, glucose and galatose. Relative molecular mass and its mass ratio were determined using high performance gel permeation chromatography (HPGPC) with the ELSD detector.

Key words: tea polysaccharide(TPS), purify, fractions of TPS, physical chemistry characteristics